Cell Electrospinning Highly Concentrated Cellular Suspensions Containing Primary Living Organisms Into Cell-Bearing Threads and Scaffolds

Jayasinghe, Suwan N.; Irvine, Scott Alexander; McEwan, Jean R.

doi:10.2217/17435889.2.4.555

Cited by 131 publications

(98 citation statements)

References 28 publications

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“…6͑c͔͒. Interestingly, the cellular viabilities reported in these studies are comparable to those cell electrospinning studies, 13,14 thus demonstrating the ability to compete in this arena with the additional advantage of not possessing the hazardous high-applied voltage.…”

Section: Resultssupporting

confidence: 65%

“…In 2005/2006, this was achieved with both these techniques, which are now referred to as "bio-electrosprays" and "cell electrospinning," demonstrating the ability to handle a wide range of living cells ͑immortalized, primary to stem cells͒ to even whole organisms. [10][11][12][13][14] Although, there has been much success with these techniques, they carry with them two significant limitations, namely, the inability to handle highly conducting suspensions while operating in stable and continuous conditions in the single needle configuration ͑in open-air ambient scenarios͒ to the hazardous driving mechanism. These obstacles, to some extent, limit the exploration of these approaches demanded for intravenous medical applications, for example, if required for repairing damaged cardiac tissue soon after myocardial infarction.…”

Section: Introductionmentioning

confidence: 99%

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Pressure driven spinning: A multifaceted approach for preparing nanoscaled functionalized fibers, scaffolds, and membranes with advanced materials

Jayasinghe

Suter²

2010

Biomicrofluidics

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Electrospinning, a flexible jet-based fiber, scaffold, and membrane fabrication approach, has been elucidated as having significance to the heath sciences. Its capabilities have been most impressive as it possesses the ability to spin composite fibers ranging from the nanometer to the micrometer scale. Nonetheless, electrospinning has limitations and hazards, negating its wider exploration, for example, the inability to handle highly conducting suspensions, to its hazardous high voltage. Hence, to date electrospinning has undergone an exhaustive research regime to a point of cliché. Thus, in the work reported herein we unveil a competing technique to electrospinning, which has overcome the above limitations and hazards yet comparable in capabilities. The fiber preparation approach unearthed herein is referred to as "pressure driven spinning ͑PDS͒." The driving mechanism exploited in this fiber spinning process is the pressurized by-pass flow. This mechanism allows the drawing of either micro-or nanosized fibers while processing polymeric suspensions containing a wide range of advanced materials spanning structural, functional, and biological entities. Similar to electrospinning if the collection time of these continuous formed fibers is varied, composite scaffolds and membranes are generated. In keeping with our interests, multicompositional structural entities such as these could have several applications in biology and medicine, for example, ranging from the development of three-dimensional cultures ͑including disease models͒ to the development of synthetic tissues and organ structures to advanced approaches for controlled and targeted therapeutics.

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Section: Resultssupporting

confidence: 65%

Section: Introductionmentioning

confidence: 99%

Pressure driven spinning: A multifaceted approach for preparing nanoscaled functionalized fibers, scaffolds, and membranes with advanced materials

Jayasinghe

Suter²

2010

Biomicrofluidics

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“…Earlier trials had involved an additional intermediate step of immersing the collector in a cross-linking solution to maintain the structural integrity and retain the cells [104], but current attempts employ thermo-responsive polymers that undergo a sol-gel transition at 37 o C, the temperature at which the cell culture medium and collector are maintained [105]. Variations in the form and number of spinnerets employed (single, coaxial or multiple) and the type of cells (human cells or micro-organisms) have also been reported [106,107]. The use of co-axial spinnerets facilitates the fabrication of distinct layers that contain different cell types, thereby leading to a functional organ.…”

Section: Living Scaffolds -The Emerging Paradigmmentioning

confidence: 99%

The Integration of Nanotechnology and Biology for Cell Engineering: Promises and Challenges

Krishnan

Sethuraman

2013

Nanomaterials and Nanotechnology

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Introduction: Successful tissue engineering strategies leading to the regeneration of a tissue depend on many factors, starting from the choice of appropriate scaffold material, tailoring the surface functionalities and topography, providing the correct amount of chemical and mechanical stimuli at the appropriate time points, and ensuring the uniform and precise localization of cells. Further challenges arise when more than one cell type has to be employed for the effective regeneration of an organ. Importance: Though the use of nanomaterials has improved tissue engineering, many pitfalls still exist that present a roadblock in the translation of tissue engineering strategies to clinical practice. Apart from employing different materials with distinct surface functionalities and mechanical properties, various strategies have been employed to manipulate the surface topography and chemistry of scaffolds to create a biomimetic microenvironment for effective tissue regeneration. Conclusion: This review provides information about the factors influencing tissue engineering, namely geometry, chemistry, mechanics and cells, and the emerging concepts that may well represent the future of regenerative medicine. Electrospinning techniques and their variants, self-assembly, cell-printing techniques and cell sheet engineering, have all been elaborated in detail. These novel techniques may serve to overcome the challenges currently faced in tissue engineering.

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“…However, in the mid 1990's electrospinning was resurrected in the laboratory as many researchers recognized its utility in the growing nanoengineering discipline (Doshi and Reneker 1995). Since then, a multitude of materials ranging from synthetic and natural polymers, ceramics, semiconductors, biomacromolecules and even cell suspensions have been electrostatically spun (Pham, Sharma et al 2006;Jayasinghe, Irvine et al 2007). Resultant fibers can be as large as 10m or as small as 5nm.…”

Section: 1 Electrospinningmentioning

confidence: 99%

Biomimetic Architectures for Tissue Engineering

Li¹,

Connell²,

Shi³

2010

Biomimetics Learning From Nature

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Cell Electrospinning Highly Concentrated Cellular Suspensions Containing Primary Living Organisms Into Cell-Bearing Threads and Scaffolds

Abstract: Our work elucidates the ability to cell electrospin primary cells as highly concentrated cellular suspensions. The post-cell electrospun organisms are viable over long periods of time, demonstrating a significant active cell population when compared with controls.

Cited by 131 publications

References 28 publications

Pressure driven spinning: A multifaceted approach for preparing nanoscaled functionalized fibers, scaffolds, and membranes with advanced materials

Pressure driven spinning: A multifaceted approach for preparing nanoscaled functionalized fibers, scaffolds, and membranes with advanced materials

The Integration of Nanotechnology and Biology for Cell Engineering: Promises and Challenges

Biomimetic Architectures for Tissue Engineering

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