Cell Division and Differentiation in Bronchial Epithelium

Ayers, Margaret; Jeffery, Peter K.

doi:10.1007/978-1-4613-3403-3_2

Cited by 14 publications

(7 citation statements)

References 18 publications

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“…Our frequent findings of transitional stages are in accordance with earlier studies on the effect of tobacco smoke on rat bronchial epithelium (7). The present findings also seem to conform with those of Ayers & Jeffery (14), who suggested that basal cells of rat bronchial epithelium differentiate to both mucous cells and ciliated cells through an ''intermediate'' cell stage. Inayama et al (15) demonstrated that purified basal cells from rabbit trachea gave rise to a fully differentiated respiratory epithelium, including GC, 4 weeks after inoculation into tracheal grafts, which had subsequently been transplanted to nude mice.…”

Section: Hyperplasia 6ersus Metaplasia In Goblet Cell Differentiationsupporting

confidence: 93%

Early Stages in the Development of Goblet Cells in the Paranasal Sinuses: A Multimethodological Study in the Rabbit

NORLANDER

1998

Acta Oto-Laryngologica

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To evaluate the pattern of goblet cell differentiation in sinus mucosa in response to external stimuli, New Zealand White rabbits were subjected to either experimental sinusitis or topical capsalcin application. Sinus mucosa was examined by light microscopy after serial sectioning, whole-mount preparation or immunohistochemistry. The mucosa was also examined by electron microscopy after perfusion fixation or high-pressure freezing. While goblet cells were normally very scarce in the healthy rabbit maxillary sinus mucosa, such cells were frequent after experimental sinusitis or topical capsaicin application. The process of goblet cell differentiation seems to follow a sequential path where serous secretory cells start to produce an increasing amount of mucous granules which appear electron lucent after conventional fixation. Parallel to this shift in secretion production, the cell assumes a bulging appearance after conventional fixation. It is concluded that newly formed goblet cells are recruited from intermediate secretory cell stages rather than from ciliated cells.

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Section: Hyperplasia 6ersus Metaplasia In Goblet Cell Differentiationsupporting

confidence: 93%

Early Stages in the Development of Goblet Cells in the Paranasal Sinuses: A Multimethodological Study in the Rabbit

NORLANDER

1998

Acta Oto-Laryngologica

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“…Changes in secretory cell number may be due to cell proliferation ('real' change) or merely changes in the amount of stainable intracellular mucin ('apparent' change) [ l o , 341 : the latter is dependent upon the balance between synthesis and secretion. Electron microscopic and autoradiographic studies show that CS generates new cells by stimulating the division of both basal and mucous cells [ 10,35,161. The acute administration of CS has also been shown to increase the output of 35S-labelled mucin into cat trachea [37].…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Cigarette Smoke-Induced Airway Secretory Cell Hyperplasia by Indomethacin, Dexamethasone, Prednisolone, or Hydrocortisone in the Rat

Rogers

Jeffery

1986

Experimental Lung Research

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As the nonsteroidal anti-inflammatory drug indomethacin has already been shown to inhibit cigarette smoke (CS)-induced secretory cell hyperplasia in the airway epithelium of the rat, the present study was undertaken to determine the effects of prophylactic interaperitoneal injection of indomethacin (4 mg/kg body weight) compared with each of three steroidal anti-inflammatory drugs given at comparable doses. The results show that dexamethasone (given initially at 4 mg/kg), prednisolone (4 mg/kg) and hydrocortisone (4 mg/kg) were also inhibitory. Dexamethasone proved to be toxic and its dose had to be reduced to give an average dose of 2 mg/kg over the experimental period. Prednisolone and hydrocortisone were toxic only when in combination with CS. The order of descending effectiveness in inhibiting secretory cell hyperplasia was indomethacin, dexamethasone, prednisolone and hydrocortisone. Indomethacin was the most effective drug in the trachea, whilst in distal intra-pulmonary airways dexamethasone was the most effective. Depending on airway level, inhibition was between 69 and 115% of the value obtained after CS alone. The inhibition was complete in all but one instance. The results show that steroids also inhibit secretory cell hyperplasia but at a comparable dose they are generally less effective than indomethacin and may have unacceptable side effects.

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“…The increased mucin mRNA steady state does not seem to occur secondary to mucous cell mitosis because mucous cells (containing electron lucent secretory granules) do not incorporate [3H]thymidine during the period of mucin mRNA induction [33]. Further, mucous cells have not been observed to incorporate [3H] thymidine during the period of rapid mucous cell increase following cigarette smoke exposure [34]. We suggest that the increased numbers of mucous cells in injured airways arise through environmental stimulation of mucin gene transcription, presumably an early event in mucous cell differentiation.…”

Section: Discussionmentioning

confidence: 99%

Mucin gene expression in rat airways following infection and irritation

Jany¹,

Gallup

Tsuda

et al. 1991

Biochemical and Biophysical Research Communications

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Airway mucus hypersecretion occurs in response to infection and irritation and poses an important and poorly understood clinical problem. In order to gain insight into its pathogenesis, we have focused on an mRNA encoding the major mucus glycoprotein, mucin. Northern blots showed that mucin mRNA was abundant in the intestine of specific pathogen free rats whereas it was undetectable in the airways of these rats until pathogen-free conditions were suspended and rats acquired Sendai (Parainfluenza I) virus infections. Airway mucin hybridization signals in rats that were both infected with Sendai virus and exposed to SO2 were more intense than those in rats with infection alone. These results suggest that pathogen-and irritant-induced hypersecretion may be partly controlled at the level of mucin mRNA.

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Cell Division and Differentiation in Bronchial Epithelium

Cited by 14 publications

References 18 publications

Early Stages in the Development of Goblet Cells in the Paranasal Sinuses: A Multimethodological Study in the Rabbit

Early Stages in the Development of Goblet Cells in the Paranasal Sinuses: A Multimethodological Study in the Rabbit

Inhibition of Cigarette Smoke-Induced Airway Secretory Cell Hyperplasia by Indomethacin, Dexamethasone, Prednisolone, or Hydrocortisone in the Rat

Mucin gene expression in rat airways following infection and irritation

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