Cell disruption and astaxanthin extraction from Haematococcus pluvialis: Recent advances

Kim, Bolam; Lee, Soo-Youn; Narasimhan, Aditya Lakshmi; Kim, Sangui; Oh, You-Kwan

doi:10.1016/j.biortech.2021.126124

Cited by 57 publications

(34 citation statements)

References 78 publications

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“…Notably, astaxanthin and lipid biosynthetic pathways in H. pluvialis are closely linked [1,4]. In addition, strigolactone is expected to enhance the germination rate of dormant cysts of H. pluvialis, similar to that observed in plant seeds [16].…”

Section: Introductionmentioning

confidence: 77%

“…The 30-d-grown seed culture was collected and inoculated to obtain an optical density (OD) of 0.1 at 680 nm (UV/Vis spectrophotometer, Optizen 3220 UV; Mecasys Co., Daejeon, Korea). Rac-GR24 was initially added at four different concentrations (2,4,6, and 8 µM) to the flasks, based on previous reports on the green microalgae C. vulgaris FACHB-8 [13] and M. sp. QLY-1 [14].…”

Section: Microalga and Photosynthetic Cultivationmentioning

confidence: 99%

“…Haematococcus pluvialis, a freshwater unicellular green microalga, is considered one of the best sources of astaxanthin (~5% of dry weight) [1,4]. Algal astaxanthin is usually synthesized as a self-defense mechanism to protect the organism from oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

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Enhancement of Astaxanthin and Fatty Acid Production in Haematococcus pluvialis Using Strigolactone

et al. 2022

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Improving the production rate of high-value nutraceutical compounds, such as astaxanthin and polyunsaturated fatty acids (PUFAs), is important for the commercialization of Haematococcus pluvialis biorefineries. Here, the effects of a phytohormone, strigolactone analog rac-GR24, on cell growth and astaxanthin and fatty acid biosynthesis in H. pluvialis were investigated. Four concentrations (2, 4, 6, and 8 µM) of rac-GR24 were initially added during 30 days of photoautotrophic cultivation. The addition of rac-GR24 improved cell number density and chlorophyll concentration in H. pluvialis cultures compared to the control; the optimal concentration was 8 µM. Despite a slightly reduced astaxanthin content of 30-d-old cyst cells, the astaxanthin production (26.1 ± 1.7 mg/L) improved by 21% compared to the rac-GR24-free control (21.6 ± 1.5 mg/L), owing to improved biomass production. Notably, at the highest dosage of 8 µM rac-GR24, the total fatty acid content of the treated H. pluvialis cells (899.8 pg/cell) was higher than that of the untreated cells (762.5 pg/cell), resulting in a significant increase in the total fatty acid production (361.6 ± 48.0 mg/L; 61% improvement over the control). The ratio of PUFAs, such as linoleic (C18:2) and linolenic (C18:3) acids, among total fatty acids was high (41.5–44.6% w/w) regardless of the rac-GR24 dose.

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Section: Introductionmentioning

confidence: 77%

Section: Microalga and Photosynthetic Cultivationmentioning

confidence: 99%

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Enhancement of Astaxanthin and Fatty Acid Production in Haematococcus pluvialis Using Strigolactone

et al. 2022

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“…This biomolecule can induce the synthesis of hyaluronan (hyaluronic acid), which is related to skin hydration [6]. Haematococcus lacustris (formerly, H. pluvialis; [7]), a unicellular freshwater green microalga, is considered an important source of astaxanthin because of its high astaxanthin content (~4% of dry weight) and potential for mass cultivation in open-pond and photobioreactor systems [8,9].…”

Section: Introductionmentioning

confidence: 99%

“…However, excessive physicochemical stress can degrade the molecular structure of astaxanthin, significantly reducing its antioxidative activity [3,17]. Therefore, efficient cell disruption and astaxanthin extraction are considered important technical issues affecting the overall economics of H. lacustris biorefineries [9,18].…”

Section: Introductionmentioning

confidence: 99%

Room-Temperature Cell Disruption and Astaxanthin Recovery from Haematococcus lacustris Cysts Using Ultrathin α-Quartz Nanoplates and Ionic Liquids

et al. 2022

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Ionic liquids (ILs) are new green solvents, which are widely used in lignocellulosic and microalgal biorefineries. However, high-temperature operating conditions limit their application in the extraction of heat-labile algal products, such as bioactive astaxanthin. In this study, we report the technical feasibility of room-temperature astaxanthin extraction from Haematococcus lacustris cysts with a thick and complex cell wall structure, by combining ultrathin α-quartz nanoplates (NPLs) with ethyl-3-methylimidazolium ([Emim])-based ILs. When four different [Emim]-based ILs with thiocyanate (SCN), diethylphosphate (DEP), HSO4, and Cl anions were applied to 90-day-old H. lacustris cysts at room temperature (~28 °C), the astaxanthin extraction efficiency was as low as 9.6-14.2%. Under sonication, α-quartz NPLs disrupted the cyst cell wall for a short duration (5 min). The astaxanthin extraction efficacies of a subsequent IL treatment improved significantly to 49.8% for [Emim] SCN, 60.0% for [Emim] DEP, 80.7% for [Emim] HSO4, and 74.3% for [Emim] Cl ions, which were 4.4, 6.1, 8.4, and 5.2 times higher than the extraction efficacy of only ILs, respectively. This finding suggests that α-quartz NPLs can serve as powerful cell-wall-disrupting agents for the room-temperature IL-mediated extraction of astaxanthin from robust algal cyst cells.

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Environmental Impacts Related to Upstream and Downstream Processing of Haematococcus pluvialis

et al. 2023

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Cell disruption and astaxanthin extraction from Haematococcus pluvialis: Recent advances

Cited by 57 publications

References 78 publications

Enhancement of Astaxanthin and Fatty Acid Production in Haematococcus pluvialis Using Strigolactone

Enhancement of Astaxanthin and Fatty Acid Production in Haematococcus pluvialis Using Strigolactone

Room-Temperature Cell Disruption and Astaxanthin Recovery from Haematococcus lacustris Cysts Using Ultrathin α-Quartz Nanoplates and Ionic Liquids

Environmental Impacts Related to Upstream and Downstream Processing of Haematococcus pluvialis

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