Cell dispersion culture for the effective growth of Humicola insolens and efficient enzyme production

Matsumoto, Hitoshi; Koganei, Keisuke; Nishida, Naoko; Koyama, Yoshiyuki; Saito, Sanshiro; Kataoka, Hideki; Ogihara, Jun; Kasumi, Takafumi

doi:10.1016/j.jbiosc.2013.08.014

Cited by 11 publications

(5 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The thermophilic fungus Humicola insolens produces an array of enzymes to degrade cellulose and hemicellulose [1,2,3,4,5]. Compared to mesophilic fungi, H. insolens exhibits a higher optimal growth temperature (45 °C), faster growth rate, and stronger secretion capacity of cellulases [6].…”

Section: Introductionmentioning

confidence: 99%

A Novel CreA-Mediated Regulation Mechanism of Cellulase Expression in the Thermophilic Fungus Humicola insolens

Fan

Song

et al. 2019

IJMS

View full text Add to dashboard Cite

The thermophilic fungus Humicola insolens produces cellulolytic enzymes that are of great scientific and commercial interest; however, few reports have focused on its cellulase expression regulation mechanism. In this study, we constructed a creA gene (carbon catabolite repressor gene) disruption mutant strain of H. insolens that exhibited a reduced radial growth rate and stouter hyphae compared to the wild-type (WT) strain. The creA disruption mutant also expressed elevated pNPCase (cellobiohydrolase activities), pNPGase (β-glucosidase activities), and xylanase levels in non-inducing fermentation with glucose. Unlike other fungi, the H. insolens creA disruption mutant displayed lower FPase (filter paper activity), CMCase (carboxymethyl cellulose activity), pNPCase, and pNPGase activity than observed in the WT strain when fermentation was induced using Avicel, whereas its xylanase activity was higher than that of the parental strain. These results indicate that CreA acts as a crucial regulator of hyphal growth and is part of a unique cellulase expression regulation mechanism in H. insolens. These findings provide a new perspective to improve the understanding of carbon catabolite repression regulation mechanisms in cellulase expression, and enrich the knowledge of metabolism diversity and molecular regulation of carbon metabolism in thermophilic fungi.

show abstract

Section: Introductionmentioning

confidence: 99%

A Novel CreA-Mediated Regulation Mechanism of Cellulase Expression in the Thermophilic Fungus Humicola insolens

Fan

Song

et al. 2019

IJMS

View full text Add to dashboard Cite

show abstract

“…Species associated with the late phase of straw decomposition were reported to be endophytic with high enzymatic activities -Chloridium aseptatum [60], Scytalidium [61,62], Colletotrichum sidae [63]. Other saprophytic fungi, found in the community, usually associated with soil or plants and reported to have high enzymatic activity, were Humicola sardiniae [64][65][66], Gibberella intricans [67,68], Albifimbria verrucaria [69], Chaetomium iranianum [70,71], Occultifur [72,73], Waitea circinata [74]. Coniochaeta, which is widely presented in the later phase, is a genus with well-known lignocellulolytic fungi [75,76].…”

Section: Discussionmentioning

confidence: 99%

The Succession of the Cellulolytic Microbial Community from the Soil during Oat Straw Decomposition

Kimeklis¹,

Gladkov²,

Orlova³

et al. 2022

Preprint

View full text Add to dashboard Cite

The decomposition of straw is a dynamic process, which is reflected in the decomposing microbial consortia, however, this process is poorly understood. Here we performed an experiment on the gradual colonization of oat straw by the soil microbial community. The results showed that soil respiration and bacterial diversity were negatively correlated – while the first decreased, the latter increased. In accordance with the dynamics of diversity and respiration, succession was divided into early, middle, and late decomposition phases. Analysis of 16S rRNA and ITS2 amplicon sequencing data revealed three jointly changing groups of phylotypes, corresponding with distinguished phases of decomposition. The presence of cellulolytic members of Proteobacteria, Bacteroidota, Firmicutes, and Actinobacteriota for bacteria and Ascomycota for fungi had been detected since the early phase, but most of the initial phylotypes were gone by the end of the phase. The second phase marked the functional core with a constant set of other phylotypes from these phyla, persisting in the community. Full metagenome sequencing of the microbial community from the end of the middle phase confirmed that major members of this consortium had GH genes, connected to cellulose and chitin degradation. The appearance of non-cellulolytic members from Bdellovibrio, Myxococcota, Chloroflexi, and Crenarchaeota characterized the last phase of the mature community.

show abstract

“…In addition, the H. insolens cellulase has stable activity at high temperature [ 2 , 3 ]. The high temperature-resistant β-glucosidase and xylanase expressed by H. insolens have been used in the wine industry for quality improvement, while its neutral cellulase has been used in the textile and washing industry [ 4 ]. By having excellent heat- and alkali-resistance, high cellulose degradation ability, and an optimal pH close to neutral, the cellulase expressed by H. insolens is a good complement to that from T .…”

Section: Introductionmentioning

confidence: 99%

“…By having excellent heat- and alkali-resistance, high cellulose degradation ability, and an optimal pH close to neutral, the cellulase expressed by H. insolens is a good complement to that from T . reesei [ 4 – 7 ]. However, the production level of H. insolens is low and cannot meet the need of biofuel industries.…”

Section: Introductionmentioning

confidence: 99%

CRISPR/Cas9-mediated genome editing directed by a 5S rRNA–tRNAGly hybrid promoter in the thermophilic filamentous fungus Humicola insolens

Fan

Zhang

et al. 2021

Biotechnol Biofuels

View full text Add to dashboard Cite

Background Humicola insolens is a filamentous fungus with high potential of producing neutral and heat- and alkali-resistant cellulase. However, the genetic engineering tools, particularly the genome-editing tool, are scarce, hindering the study of cellulase expression regulation in this organism. Results Herein, a CRISPR/Cas9 genome-editing system was established in H. insolens based on a hybrid 5S rRNA–tRNAGly promoter. This system is superior to the HDV (hepatitis delta virus) system in genome editing, allowing highly efficient single gene destruction in H. insolens with rates of deletion up to 84.1% (37/44). With this system, a putative pigment synthesis gene pks and the transcription factor xyr1 gene were disrupted with high efficiency. Moreover, the extracellular protein concentration and cellulase activity largely decreased when xyr1 was deleted, demonstrating for the first time that Xyr1 plays an important role in cellulase expression regulation. Conclusions The established CRISPR/Cas9 system is a powerful genetic operation tool for H. insolens, which will accelerate studies on the regulation mechanism of cellulase expression and engineering of H. insolens for higher cellulase production.

show abstract

Cell dispersion culture for the effective growth of Humicola insolens and efficient enzyme production

Cited by 11 publications

References 10 publications

A Novel CreA-Mediated Regulation Mechanism of Cellulase Expression in the Thermophilic Fungus Humicola insolens

A Novel CreA-Mediated Regulation Mechanism of Cellulase Expression in the Thermophilic Fungus Humicola insolens

The Succession of the Cellulolytic Microbial Community from the Soil during Oat Straw Decomposition

CRISPR/Cas9-mediated genome editing directed by a 5S rRNA–tRNAGly hybrid promoter in the thermophilic filamentous fungus Humicola insolens

Contact Info

Product

Resources

About