Cell Death Suggestive of Apoptosis After Spinal Cord Ischemia in Rabbits

Mackey, Mary E.; Wu, Yingji; Hu, Rong; Demaro, Joseph A.; Jacquin, Mark F.; Kanellopoulos, Georgios K.; Hsu, Chung Y.; Kouchoukos, Nicholas T.

doi:10.1161/01.str.28.10.2012

Cited by 68 publications

(38 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The TUNEL labeling present in ventral horn motor neurons in the saline-treated group, which was almost entirely absent in the rHu-EPO-treated groups, is strongly supportive of an effect of EPO in blocking the induction of an apoptotic program in this class of neurons. Recent studies using this rabbit model have shown that within the first few days following transient spinal cord global ischemia the motor neurons undergo apoptosis (27,28). The results observed here, therefore, confirm these observations as well as demonstrate that rHu-EPO can prevent ischemia-induced programmed cell death.…”

Section: Discussionsupporting

confidence: 89%

Erythropoietin prevents motor neuron apoptosis and neurologic disability in experimental spinal cord ischemic injury

Çeli̇k

Gökmen

Erbayraktar

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

433

275

View full text Add to dashboard Cite

The cytokine erythropoietin (EPO) possesses potent neuroprotective activity against a variety of potential brain injuries, including transient ischemia and reperfusion. It is currently unknown whether EPO will also ameliorate spinal cord injury. Immunocytochemistry performed using human spinal cord sections showed abundant EPO receptor immunoreactivity of capillaries, especially in white matter, and motor neurons within the ventral horn. We used a transient global spinal ischemia model in rabbits to test whether exogenous EPO can cross the blood-spinal cord barrier and protect these motor neurons. Spinal cord ischemia was produced in rabbits by occlusion of the abdominal aorta for 20 min, followed by saline or recombinant human (rHu)-EPO (350, 800, or 1,000 units/kg of body weight) administered intravenously immediately after the onset of reperfusion. The functional neurological status of animals was better for rHu-EPO-treated animals 1 h after recovery from anesthesia, and improved dramatically over the next 48 h. In contrast, saline-treated animals exhibited a poorer neurological score at 1 h and did not significantly improve. Histopathological examination of the affected spinal cord revealed widespread motor neuron injury associated with positive terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling in control but not in rHu-EPO-treated animals. These observations suggest both an acute as well as a delayed beneficial action of rHu-EPO in ischemic spinal cord injury. Because rHu-EPO is currently used widely with an excellent safety profile, clinical trials evaluating its potential to prevent motor neuron apoptosis and the neurological deficits that occur as a consequence of ischemic injury are warranted.

show abstract

Section: Discussionsupporting

confidence: 89%

Erythropoietin prevents motor neuron apoptosis and neurologic disability in experimental spinal cord ischemic injury

Çeli̇k

Gökmen

Erbayraktar

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

433

275

View full text Add to dashboard Cite

show abstract

“…This information can be applied not only to domestic animals but also to humans (Cizkova et al, 2001). Furthermore, as an excellent model species, rabbits help to investigate such conditions like arterial injury (Jorgensen et al, 1972), aortic occlusion (Mackey et al, 1997) and aortic aneurysm (Freestone et al, 1997). However, there is limited information about basic anatomy of the rabbit kidney.…”

Section: Introductionmentioning

confidence: 99%

Morphometry and Vascularization of the Rabbit Kidneys (Oryctolagus cuniculus)

et al. 2015

View full text Add to dashboard Cite

SUMMARY: Rabbits have been used as urologic models in many studies. The aim of this study was to characterize the renal morphology and anatomical variations of the renal pedicle in rabbit. The research ethics committee of Rio de Janeiro Federal Rural University approved this study. The animals were obtained from the university's necropsy unit. Dissections were performed in 50 adult rabbits, male and females, without macroscopic renal pathology. Kidney measurements were made with a digital caliper: length, width, and thickness. The length and origin of the renal arteries and main branches and length of the renal veins were also determined. The terminology of Nomina Anatomica Veterinaria was followed. The mean and standard deviation of the length of the right kidney was 3.150±0.2588 cm in males and 3.127±0.3769 cm in females and in the left kidney was 3.083±0.0818 cm in males and 3.162±0.0848 cm in females. The width of the right kidney was 1.933±0.0848 cm in males and 1.996±0.0680 cm in females and in the left kidney was 1.850±0.0659 cm in males and 2.004±0.0940 cm in females. There was no significant difference in the measurements between the two sexes and antimeres and in the measures of renal vessels in relation to sex, but the left artery and renal vein were always larger than the right in both sexes. The results of the present study are expected to contribute to the body of knowledge in the field of comparative and applied anatomy.

show abstract

“…All of these risk factors may alter spinal cord blood supply, and finally causing apoptosis and necrosis of neurons [13,14]. Cord dysfunction occurs and even worsens though postoperative immediate neurologic function improves.…”

Section: Disscussionmentioning

confidence: 99%

Delayed Neurologic Deficit after Thoracolumbar Kyphosis Correction: One Case Report

Rong¹

2015

J Spine

View full text Add to dashboard Cite

Study design: Case Report.Objective: To present one case suffering from delayed neurologic deficit after kyphosis deformity correction and analyze its potential etiological factors, how to identify and treat it.Summary of background data: Tardive neurologic deficits occur much less than acute ones after corrections of spinal deformities. Currently some risk factors, such as postoperative hypotension, deformity severity, and intraoperative haemorrhage are considered to be related with its occurrence and worsening though the true mechanisms are not clear. Extrinsic cord compression should be excluded by radiographic examinations before conservative treatments, such as improving blood perfusion, eliminating cord edema, suppressing local inflammation; promoting neurite outgrowth can be applied. If these don't lead to functional recovery of spinal cord, emergency operation returning the cord to its former kyphotic position can also be considered. Methods:This 44 year-old female patient demonstrated thoracolumbar kyphosis of 136.9° combined with incompetence of spinal cord before operation, and underwent posterior vertebra column resection with the kyphotic correction of 68.8°. No neurologic dysfunction was found during operation.

show abstract

Cell Death Suggestive of Apoptosis After Spinal Cord Ischemia in Rabbits

Abstract: Spinal cord ischemia in rabbits induces morphological and biochemical changes suggestive of apoptosis. These data raise the possibility that apoptosis contributes to neuronal cell death after spinal cord ischemia.

Cited by 68 publications

References 34 publications

Erythropoietin prevents motor neuron apoptosis and neurologic disability in experimental spinal cord ischemic injury

Erythropoietin prevents motor neuron apoptosis and neurologic disability in experimental spinal cord ischemic injury

Morphometry and Vascularization of the Rabbit Kidneys (Oryctolagus cuniculus)

Delayed Neurologic Deficit after Thoracolumbar Kyphosis Correction: One Case Report

Contact Info

Product

Resources

About