Cell death induced by the phenolic antioxidant tert-butylhydroquinone and its metabolite tert-butylquinone in human monocytic leukemia U937 cells

Okubo, Tomoko; Yokoyama, Yuka; Kanô, Kiyoshi; Kano, Itsu

doi:10.1016/s0278-6915(03)00002-4

Cited by 82 publications

(56 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The activation of the MPT results in the initiation of apoptotic processes. In U937 monocytes, tBHQ altered mitochondrial membrane potential and resulted in the release of cytochrome c (Okubo et al 2003). This is another process where tBHQ-mediated injury and Trx2 function intersects and may relate to factors involved in Nrf2 activation and signaling.…”

Section: Discussionmentioning

confidence: 97%

“…Conversely, overexpression of C93S Trx2 potentiated tBHQ-mediated induction of the expression of all three genes. Asterisks denote a statistically significant difference (p<0.05) from tBHQ-treated empty vector control cultures and the Trx2 or C93S Trx2 transfected cultures contradictory, where tBHQ induces a decrease in GSH in certain cell types but causes an increase in others (Liu et al 1994;Nakagawa et al 1994;Nakagawa 1996;Hara et al 2003;Okubo et al 2003). While our studies did not show a very profound effect on the GSH system, there have been reports of tBHQ-related GSH conjugates in animal models, demonstrating tBHQ's interaction with GSH (Peters et al 1996).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Tert-butylhydroquinone induces mitochondrial oxidative stress causing Nrf2 activation

Imhoff

Hansen

2010

Cell Biol Toxicol

View full text Add to dashboard Cite

Tert-butylhydroquinone (tBHQ), the major metabolite of butylated hydroxyanisole, induces an antioxidant response through the redox-sensitive transcription factor, nuclear factor-E2-related factor-2 (Nrf2). However, the mechanism by which tBHQ induces Nrf2 activity is not entirely understood. Here, we show that tBHQ preferentially alters the redox status in the mitochondrial compartment in HeLa cells. HeLa cells treated with tBHQ showed a preferential oxidation of mitochondrial thioredoxin-2 (Trx2), while cellular glutathione and cytosolic thioredoxin-1 were not affected. Preferential mitochondrial oxidation by tBHQ was supported by detection of reactive oxygen species (ROS) specific to this compartment. To determine the role of Trx2 in regulating downstream effects of tBHQ, HeLa cells were transiently transfected with an empty, Trx2, or C93S (Cys93Ser) Trx2 dominant-negative mutant expression vector. Overexpression of Trx2 decreased basal mitochondrial ROS production, whereas expression of C93S Trx2 enhanced it. In addition, under untreated conditions, expression of C93S Trx2 led to an increase in the basal activities of Nrf2. With tBHQ treatments, Trx2 overexpression suppressed Nrf2 accumulation and activity, whereas expression of C93S Trx2 had no effect on the degree of inducibility or Nrf2 accumulation but did increase the overall activity of Nrf2. Quantitative polymerase chain reaction analysis of Nrf2-regulated gene expression corroborate Trx2 control of tBHQ-mediated Nrf2 activation. These data show a compartment-specific effect where tBHQ-induced Nrf2 signaling is mediated by Trx2 and suggest that antioxidant status in various compartments would provide different levels of control of redox signaling.

show abstract

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Tert-butylhydroquinone induces mitochondrial oxidative stress causing Nrf2 activation

Imhoff

Hansen

2010

Cell Biol Toxicol

View full text Add to dashboard Cite

show abstract

“…En la industria alimentaria se han utilizado antioxidantes sintéticos como butil-hidroxitolueno (BHT), butil-hidroxianisol (BHA) y terbutil-hidroquinona (TBHQ) para retardar la oxidación de lípidos (Fasseas et al, 2007). Sin embargo, existe preocupación por la utilización de estos antioxidantes sintéticos por su riesgo de toxicidad (Okubo et al, 2003;Rodrí-guez et al 2012).…”

Section: Introductionunclassified

ESTABILIDAD DURANTE EL ALMACENAMIENTO DE CARNE DE POLLOS ALIMENTADOS CON ORÉGANO SECO (Origanum vulgare L.) EN LA DIETA

Velasco

Bravo

Williams

et al. 2017

Chil. j. agric. anim. sci.

View full text Add to dashboard Cite

RESUMENLos antioxidantes naturales pueden ser usados para aumentar la estabilidad de la carne de pollo durante el almacenamiento. El objetivo de este estudio fue determinar la estabilidad durante el almacenamiento de carne de pollos alimentados con una dieta suplementada con orégano seco en dosis 0; 1,25; 2,5; 3,75 y 5% con respecto a la materia seca ofrecida. Los parámetros evaluados durante el almacenamiento de la carne fueron: índice de peróxidos, recuento de aerobios mesófilos (RAM), color (espacio CIELab), composición nutricional, características sensoriales (color, intensidad de aroma) y aceptación. Se encontró que la carne de pollos alimentados con dietas suplementadas con orégano seco presentó una menor oxidación de lípidos al inicio del almacenamiento en refrigeración, mayores diferencias de color (∆E > 5) con respecto al control en los tratamientos con mayor inclusión de orégano (2,5-5%) a los siete días de almacenamiento, un RAM sin variación entre tratamientos, un mayor contenido de lípidos en tratamientos con mayor inclusión de orégano (3,75 y 5%), y una mayor aceptación por el consumidor. Se puede concluir que la estabilidad de la carne de pollos alimentados con orégano seco como ingrediente dietario aumenta al inicio del almacenamiento por la menor oxidación de lípidos.Palabras clave: antioxidantes, índice de peróxidos, recuento total, espacio CIELab, análisis sensorial. ABSTRACTNatural antioxidants may be used in order to increase storage stability of poultry meat. The aim of this study was to determine the storage stability of poultry meat from broilers fed with diets containing dry oregano at different doses 0, 1.25, 2.5, 3.75 and 5%. The following parameters were evaluated: peroxides index, total mesophilic aerobic count (RAM), color (CIELab), nutritional composition, sensory characteristics (color and aroma intensity) and consumer acceptance. The results showed that poultry meat from broilers fed with dry oregano exhibited a lower oxidation of lipids at the beginning of refrigerated storage. Regarding color, higher differences (∆E > 5) were observed in the treatments with higher doses of oregano (2.5-5%) seven days after storage when compared with the control. No variation in TMA values were found between treatments. Lipid content was higher in the treatments with a higher amount of oregano added (3.75 and 5%), while consumer acceptance 94-101.

show abstract

“…This substance is able to significantly obstruct or inhibit the oxidation of a substance that is easily oxidized even in low concentrations, not more than 0.02% of oil or fat contained in food [2]. TBHQ is formed by BHA through an O-methylated process [3]. TBHQ is a preservative in products of fats and oils; animal fat; margarine and similar products; snacks made from potatoes, cereals, flour and starch; and nut products [3].…”

Section: Introductionmentioning

confidence: 99%

“…TBHQ is a preservative in products of fats and oils; animal fat; margarine and similar products; snacks made from potatoes, cereals, flour and starch; and nut products [3]. Although the use of preservatives such as BHA and TBHQ is permitted by the WHO with BHA acceptable daily intake (ADI) of 0.5 mg/kg body weight [3], and TBHQ at 0.70 mg/kg body weight [4][5][6] until now, BHA and TBHQ acceptable consumption still makes controversy because several studies reported that BHA and TBHQ could damage DNA, leading to initiate and promote tumors in several animal tissues. TBHQ can cause the breakdown of DNA in vitro [7] and form 8-hydroxy-2'-deoxyguanosine on Calf Thymus DNA [8].…”

Section: Introductionmentioning

confidence: 99%

The study of DNA adduct 8-hydroxy-2′deoxyguanosine (8-OHdG) formation of butylated hydroxyanisole (BHA) and its metabolite ter-butyl hydroquinone (TBHQ) through in vitro reaction with Calf Thymus DNA and 2′deoxyguanosine

Budiawan

Purwaningsih

Cahaya

2017

IOP Conf. Ser.: Mater. Sci. Eng.

View full text Add to dashboard Cite

View the article online for updates and enhancements. Related contentStudy of DNA adduct 8 hydroxy-2'-deoxyguanosine (8-OHdG) formation through fenton reaction with tertbutylhydroquinone (TBHQ) and butyl hydroxy toluene (BHT) S Handayani, I C Dani, Budiawan et al.-DNA interaction studies of new nano metal based anticancer agent: validation byspectroscopic methods Sartaj Tabassum, Girish Chandra Sharma, Farukh Arjmand et al. Although WHO declared their safety, the use of these preservatives are still controversial because some studies showed that BHA induced proliferative effects in animal testing and TBHQ is considered as carcinogenic and causes DNA cleavage. This study is aimed to analyze the interaction between Calf Thymus DNA with BHA and TBHQ which are mediated with Copper (II) Chloride. The result of the study in spectrophotometric showed there was bathochromic shift as much as 2-3 nm in DNA treated with TBHQ. The next analysis used HPLC method in stationary phase of ODS, mobile phase of 10mM Natrium Hydrogen Phosphate Buffer and Methanol (85 : 15) for DNA adduct formation, 8-Hydroxy-2-Deoxyguanosine (8-OHDG) as biomarker of risk cancer. The resultof the study showed the formation of DNA adduct 8-OHDG in the interaction between DNA and 20-500 ppm of TBHQ. The 8-OHdG formation was greatly increased by the higher concentration of TBHQ. The relative amount of 8 OHDG which formed was reached 946/10 5 deoxyguanosine in DNA bases. Confirmation test by LCMS/MS was characterized with the detection of mother ion peak (m/z 284); fragment ion peaks at m/z 167.9, and 139.9; at retention time 3.52 min. Meanwhile the interaction between DNA and 50-250 ppm BHA did not induce 8-OHDG.

show abstract

Cell death induced by the phenolic antioxidant tert-butylhydroquinone and its metabolite tert-butylquinone in human monocytic leukemia U937 cells

Cited by 82 publications

References 34 publications

Tert-butylhydroquinone induces mitochondrial oxidative stress causing Nrf2 activation

Tert-butylhydroquinone induces mitochondrial oxidative stress causing Nrf2 activation

ESTABILIDAD DURANTE EL ALMACENAMIENTO DE CARNE DE POLLOS ALIMENTADOS CON ORÉGANO SECO (Origanum vulgare L.) EN LA DIETA

The study of DNA adduct 8-hydroxy-2′deoxyguanosine (8-OHdG) formation of butylated hydroxyanisole (BHA) and its metabolite ter-butyl hydroquinone (TBHQ) through in vitro reaction with Calf Thymus DNA and 2′deoxyguanosine

Contact Info

Product

Resources

About