Cell Death in Cells Overlying Lateral Root Primordia Facilitates Organ Growth in Arabidopsis

Escamez, Sacha; André, Domenique; Sztojka, Bernadette; Bollhöner, Benjamin; Hall, Hardy; Berthet, Béatrice; Voß, Ute; Lers, Amnon; Maizel, Alexis; Andersson, Magnus; Bennett, Malcolm; Tuominen, Hannele

doi:10.1016/j.cub.2019.11.078

Cited by 41 publications

(39 citation statements)

References 70 publications

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“…The importance of cell-cell communication is also exemplified by the process of LR development. Since LR development initiates from a subset of pericycle cells located deep within the primary root [ 146 ], LR growth and emergence from the root surface require the cooperation of the three overlying cell layers (the endoderm, cortex, and epidermis) [ 147 , 148 , 149 ]. Communication between pericycle and endoderm cells occurs prior to the first cell division of the LR founder cells [ 149 , 150 ].…”

Section: The Roles Of Pectin In Cell–cell Communicationmentioning

confidence: 99%

Recent Advances in Understanding the Roles of Pectin as an Active Participant in Plant Signaling Networks

Shin

Chane

Jung

et al. 2021

Plants

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Pectin is an abundant cell wall polysaccharide with essential roles in various biological processes. The structural diversity of pectins, along with the numerous combinations of the enzymes responsible for pectin biosynthesis and modification, plays key roles in ensuring the specificity and plasticity of cell wall remodeling in different cell types and under different environmental conditions. This review focuses on recent progress in understanding various aspects of pectin, from its biosynthetic and modification processes to its biological roles in different cell types. In particular, we describe recent findings that cell wall modifications serve not only as final outputs of internally determined pathways, but also as key components of intercellular communication, with pectin as a major contributor to this process. The comprehensive view of the diverse roles of pectin presented here provides an important basis for understanding how cell wall-enclosed plant cells develop, differentiate, and interact.

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Section: The Roles Of Pectin In Cell–cell Communicationmentioning

confidence: 99%

Recent Advances in Understanding the Roles of Pectin as an Active Participant in Plant Signaling Networks

Shin

Chane

Jung

et al. 2021

Plants

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“…This third population also had the highest expression of the NAC transcription factor, ORESARA1 ( ORE1 ) and autophagy marker gene ATG8a ( Figure 5D and E ). ORE1 is a positive regulator of programmed cell death and autophagy marker gene ATG8a are specifically expressed in cells overlying LRPs (Escamez et al, 2020). ORE1 expressing cells eventually die in order to make space for the lateral root to emerge (Escamez et al, 2020).…”

Section: Resultsmentioning

confidence: 99%

“…ORE1 is a positive regulator of programmed cell death and autophagy marker gene ATG8a are specifically expressed in cells overlying LRPs (Escamez et al, 2020). ORE1 expressing cells eventually die in order to make space for the lateral root to emerge (Escamez et al, 2020). As such, we categorized these three populations as cortex (127 cells), endodermis (410 cells), and lateral root-responding endodermis (LRE) (276 cells) ( Supplemental Table 1, Supplemental Figure 10A ).…”

Section: Resultsmentioning

confidence: 99%

“…These cells were enriched in the expression of genes falling in ontology categories for hormone and auxin response, cell-cell communication, and lateral root development, making a strong case that they are responding to developing primordia in underlying pericycle cells. They were also enriched in ORE1 expression, a gene that has recently been shown to play a role in lateral root initiation and emergence through programmed cell death of tissue overlying LRPs (Escamez et al, 2020). Consequently, this population of endodermal cells appears to be responding to lateral root initiation in neighboring pericycle cells, and forging a path for the incipient primordium.…”

Section: Discussionmentioning

confidence: 99%

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A single cell view of the transcriptome during lateral root initiation inArabidopsis thaliana

Gala

Lanctot

Jean-Baptiste

et al. 2020

Preprint

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Root architecture is a major determinant of fitness, and is under constant modification in response to favorable and unfavorable environmental stimuli. Beyond impacts on the primary root, the environment can alter the position, spacing, density and length of secondary or lateral roots. Lateral root development is among the best-studied examples of plant organogenesis, yet there are still many unanswered questions about its earliest steps. Among the challenges faced in capturing these first molecular events is the fact that this process occurs in a small number of cells with unpredictable timing. Single-cell sequencing methods afford the opportunity to isolate the specific transcriptional changes occurring in cells undergoing this fate transition. Using this approach, we successfully captured the transcriptomes of initiating lateral root primordia, and discovered many previously unreported upregulated genes associated with this process. We developed a method to selectively repress target gene transcription in the xylem pole pericycle cells where lateral roots originate, and demonstrated that expression of several of these targets was required for normal root development. We also discovered novel subpopulations of cells in the pericycle and endodermal cell files that respond to lateral root initiation, highlighting the coordination across cell files required for this fate transition.

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“…The outer cortex is subject to enormous mechanical constraint in the rootlet emergence zone due to the growing primordium. Cell death can be observed in the endodermis and to a lesser extent in the cortex cells in species such as Arabidopsis (Escamez et al, 2020). In white lupin, those layers (endodermis and inner cortex) are dividing and become included within the rootlet primordium.…”

Section: Discussionmentioning

confidence: 99%

Auxin and pectin remodeling interplay during rootlet emergence in white lupin

Jobert

Soriano

Brottier

et al. 2021

Preprint

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Secondary root emergence is a crucial trait that shapes the plants underground system. Virtually every developmental step of root primordium morphogenesis is controlled by auxin. However, how the hormone controls cell separation in primordium-overlaying tissues through wall loosening is poorly understood. Here, we took advantage of white lupin and its spectacular cluster root development to assess the contribution of auxin to this process. We show that auxin positive role on rootlet emergence is associated with an upregulation of cell wall pectin modifying and degrading genes. Downregulation of a pectinolytic enzyme gene expressed in cells surrounding the primordium resulted in delayed emergence. Pectins were demethylesterified in the emergence zone and auxin treatment further enhanced this effect. Additionally, we report specific rhamnogalacturonan-I modifications during cortical cell separation. In conclusion, we propose a model in which auxin has a dual role during rootlet emergence: Firstly, through active pectin demethylesterification and secondly by regulating the expression of cell wall remodeling enzymes.

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Cell Death in Cells Overlying Lateral Root Primordia Facilitates Organ Growth in Arabidopsis

Cited by 41 publications

References 70 publications

Recent Advances in Understanding the Roles of Pectin as an Active Participant in Plant Signaling Networks

Recent Advances in Understanding the Roles of Pectin as an Active Participant in Plant Signaling Networks

A single cell view of the transcriptome during lateral root initiation inArabidopsis thaliana

Auxin and pectin remodeling interplay during rootlet emergence in white lupin

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