Cell Death in Cells Overlying Lateral Root Primordia Contributes to Organ Growth in  &lt;i&gt;Arabidopsis&lt;/i&gt;

Escamez, Sacha; André, Domenique; Sztojka, Bernadette; Bollhöner, Benjamin; Hall, Hardy; Berthet, Béatrice; Voß, Ute; Lers, Amnon; Maizel, Alexis; Andersson, Magnus; Bennett, Malcolm; Tuominen, Hannele

doi:10.2139/ssrn.3472040

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“…Cell wall structure is integral to lateral root emergence since the LRP must move through multiple cell layers requiring tightly controlled cell wall remodeling and mutants with impaired cell wall modifying enzymes have altered development (76)(77)(78). The cells within the primordia need to have strong cell wall structure to push through cell layers, and the cells overlying the primordia need to have their cell wall loosened to allow for emergence (76,78). Therefore, one possible scenario is the decrease in the superoxide pool within the LRP in tt7-2 may contribute to a less dense cell wall within the LRP impairing lateral root emergence.…”

Section: Discussionmentioning

confidence: 99%

Flavonols modulate lateral root emergence by scavenging reactive oxygen species inArabidopsis thaliana

Chapman

Muday

2020

Preprint

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Flavonoids are plant-specific antioxidant compounds that modulate plant development, which include flavonols and anthocyanins subclasses. In Arabidopsis thaliana, mutants in genes encoding each step in the flavonoid biosynthetic pathway have been isolated. We used these mutants to examine the role of flavonols in initiation and emergence of lateral roots and asked whether this regulation occurs through scavenging ROS. The tt4 mutants have a defect in the first committed step of flavonoid biosynthesis and have increased lateral root emergence. This phenotype was reversed by both genetic and chemical complementation. Using these flavonoid biosynthetic mutants, we eliminated roles for anthocyanins and the flavonols, quercetin and isorhamnetin, in controlling lateral root development. The tt7-2 mutant has a defect in a branchpoint enzyme blocking quercetin biosynthesis that led to elevated levels of kaempferol and reduced lateral roots. Kaempferol accumulated within lateral root primordia and was significantly increased in tt7-2. Thee data are consistent with kaempferol acting as a negative regulator of lateral root emergence. We examined ROS accumulation above and within the primordia using a general ROS sensor and identified increased signal above the primordia of the tt4 and tt7-2 mutants compared to wild type. Using a superoxide specific sensor, we detected a decrease in signal within the primordia of tt7-2, but not the tt4 mutant, compared to wild type. Together, these results support a model in which increased level of kaempferol in tt7-2 leads to a reduction in superoxide concentration in the lateral root primordia thereby reducing ROS-stimulated lateral root emergence.

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