Cell death during natural and induced luteal regression in mares

Al-Zi'abi, MO; Fraser, H. M.; Watson, E.D.

doi:10.1530/rep.0.1230067

Cited by 41 publications

(22 citation statements)

References 11 publications

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“…Morphological alterations of both steroidogenic cells and blood vessels are essential for regression of cyclic CL [8], with microvascular endothelial cells being the first ones to undergo apoptosis [58]. Since in the mare, overall apoptosis also increases in the late luteal phase CL [59,60], it makes sense that in this study vascular regression was also increased in this stage of the luteal phase, as shown by a decrease in both microvascular areas and endothelial cell mitogenesis under the influence of PGF 2␣ . In conclusion, vascular growth and regression appear to be coordinated with development of non vascular tissue.…”

Section: Discussionmentioning

confidence: 99%

Microvascularization and angiogenic activity of equine corpora lutea throughout the estrous cycle

Ferreira‐Dias¹,

Pinto‐Bravo²,

Mateus³

et al. 2006

Domestic Animal Endocrinology

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Corpus luteum growth and endocrine function are closely dependent on the formation of new capillaries. The objectives of this study were to evaluate (i) tissue growth and microvascular development in the equine cyclic luteal structures; (ii) in vitro angiogenic activity of luteal tissues in response to luteotrophic (LH, PGE 2 ) and luteolytic (PGF 2␣ ) hormones and (iii) to relate data to luteal endocrinological function. Our results show that microvascular density was increased in the early and mid luteal phase, followed by a fall in the late luteal phase and a further decrease in the corpus albicans. Hyperplasia of luteal tissue increased until the mid luteal phase and it was followed by tissue regression. Luteal explants were cultured with no hormone added, or with PGF 2␣ , LH, PGE 2 , LH + PGE 2 or LH + PGF 2␣ . Media conditioned by equine luteal tissue from different stages of the luteal phase were able to stimulate mitogenesis of bovine aortic endothelial cells (BAEC), suggesting the presence of angiogenic activity. No difference was observed among luteal structures on their mitogenic capacity, for any treatment used. Nevertheless, Late-CL conditioned-media with PGF 2␣ showed a significant decrease in BAEC proliferation (p < 0.05) and LH + PGF 2␣ a tendency to reduce mitogenesis. Thus, prostaglandin F 2␣ may play a role on vascular regression of the CL during the late luteal phase in the * Corresponding author. Tel.: +351 213652859; fax: +351 213652889. -mail address: gmlfdias@fmv.utl.pt (G. Ferreira-Dias Ferreira-Dias et al. / Domestic Animal Endocrinology 30 (2006) [247][248][249][250][251][252][253][254][255][256][257][258][259] mare. These data suggest that luteal angiogenesis and vascular regression in the mare are coordinated with the development of non-vascular tissue and might be regulated by many different factors. E

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Section: Discussionmentioning

confidence: 99%

Microvascularization and angiogenic activity of equine corpora lutea throughout the estrous cycle

Ferreira‐Dias¹,

Pinto‐Bravo²,

Mateus³

et al. 2006

Domestic Animal Endocrinology

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“…Detection of neutrophils and macrophages in the CL using the periodic acid-Schiff reaction Formalin-fixed sections (5 mm) of the luteal tissue samples were stained with periodic acid-Schiff (PAS) reagent (Sigma) for 10 min and then counterstained with haematoxylin as previously described (Al-Zi'abi et al 2002, Jiemtaweeboon et al 2011. In general, it is recognised that PAS staining is a useful method to detect granulophilic leukocytes.…”

Section: Western Blottingmentioning

confidence: 99%

Possible role of interferon tau on the bovine corpus luteum and neutrophils during the early pregnancy

et al. 2015

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When pregnancy is established, interferon tau (IFNT), a well-known pregnancy recognition signal in ruminants, is secreted by embryonic trophoblast cells and acts within the uterus to prepare for pregnancy. IFNT acts as an endocrine factor on the corpus luteum (CL) to induce refractory ability against the luteolytic action of PGF 2a . Hypothesising that IFNT may influence not only the uterine environment but also the CL in cows via local or peripheral circulation, we investigated qualitative changes in the CL of pregnant cows during the maternal recognition period (day 16) and the CL of non-pregnant cows. The CL of pregnant animals had a higher number of neutrophils, and the expression of interleukin 8 (IL8) mRNA and its protein was higher as well as compared with the CL of non-pregnant animals. Although IFNT did not affect progesterone (P 4 ) secretion and neutrophil migration directly, it stimulated IL8 mRNA expression on luteal cells (LCs), influencing the neutrophils, resulting in the increased migration of IFNT-activated neutrophils. Moreover, both IFNT-activated neutrophils and IL8 increased P 4 secretion from LCs in vitro. Our novel finding was the increase in neutrophils and IL8 within the CL of pregnant cows, suggesting the involvement of IFNT function within the CL toward establishment of pregnancy in cows. The present results suggest that IFNT upregulates neutrophil numbers and function via IL8 on LCs in the CL of early pregnant cows and that both neutrophils and IL8, stimulated by IFNT, are associated with an increase in P 4 concentrations during the maternal recognition period in cows. Reproduction (2015) 150 217-225

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“…It is determinant for normal reproductive function and development in the ovary [11]. This process has been reported in CL involution in several species, such as mouse [12,13], rat [14,15], rabbit [16,17], sheep [18,19], cow [20,21], pig [22], human [23], and mare [24,25].…”

Section: Introductionmentioning

confidence: 96%

Is FAS/Fas Ligand System Involved in Equine Corpus Luteum Functional Regression?1

Galvão¹,

Ramilo²,

Skarzynski³

et al. 2010

Biology of Reproduction

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Proapoptotic factor Fas ligand (FASL) and its cell surface receptor FAS are tumor necrosis factor superfamily members that trigger apoptosis in different cell types. However, their influence on luteal steroidogenesis is not clearly understood. The aim of the present work was to determine (i) the presence of the cytokine FASL and its receptor FAS in the mare's corpus luteum (CL) throughout the luteal phase, as well as (ii) the influence of FASL alone, or together with the cytokines tumor necrosis factor alpha (TNF) and interferon gamma (IFNG), on equine luteal cell production of luteotrophic and luteolytic factors, cell viability, and apoptosis. FASL and FAS protein expression and mRNA transcription were evaluated in different luteal stages of the equine CL by Western blotting and real-time PCR assays, respectively. Protein expression and FASL mRNA transcription increased in the late CL. Also, FAS and FASL proteins were present in large steroidogenic and endothelial CL cells throughout the luteal phase, as demonstrated by immunohistochemistry. Equine luteal cells isolated from midluteal phase CL were stimulated without (control) or with exogenous cytokines: FASL (10 ng/ml); TNF+IFNG (10 ng/ml each; positive control) or FASL+TNF+IFNG (10 ng/ml each). FASL clearly inhibited in vitro progesterone and prostaglandin E(2) (PGE(2)) production by equine luteal cells but increased prostaglandin F(2alpha) (PGF(2alpha)). Furthermore, FASL effect on equine luteal cell viability depended on the presence of cytokines TNF and IFNG. In conclusion, this study shows the presence of FASL and FAS in the equine CL and suggests their importance in functional luteolysis.

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Cell death during natural and induced luteal regression in mares

Cited by 41 publications

References 11 publications

Microvascularization and angiogenic activity of equine corpora lutea throughout the estrous cycle

Microvascularization and angiogenic activity of equine corpora lutea throughout the estrous cycle

Possible role of interferon tau on the bovine corpus luteum and neutrophils during the early pregnancy

Is FAS/Fas Ligand System Involved in Equine Corpus Luteum Functional Regression?1

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