Cell Cytometry: Review and Perspective on Biotechnological Advances

Vembadi, Abhishek; Menachery, Anoop; Qasaimeh, Mohammad A.

doi:10.3389/fbioe.2019.00147

Cited by 116 publications

(72 citation statements)

References 129 publications

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“…Early medical practitioners realized the importance of counting blood cells as a tool for investigation and quantitative study in healthcare [ 187 ]. For over 150 years, cell biologists had been using a hemocytometer (counting chambers) to quantify cells (mm).…”

Section: The Potential Use Of Iace For Extracellular Vesicle Studimentioning

confidence: 99%

“…For over 150 years, cell biologists had been using a hemocytometer (counting chambers) to quantify cells (mm). Today, automated instruments such as flow cytometers are widely used in research and for management of patient diagnosis and prognosis, particularly for blood cancer cells [ 187 , 188 ]. Significant effort has been made to analyze cellular and subcellular entities, as well as viral particles and cellular vesicles using a flow cytometer (recently dubbed “flow virometry”) [ 189 ], but the straightforward applicability of this technique has been hampered by the small sizes of the particles and vesicles, their polydispersity, and the low refractive index [ 190 , 191 , 192 ].…”

Section: The Potential Use Of Iace For Extracellular Vesicle Studimentioning

confidence: 99%

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A Two-Dimensional Affinity Capture and Separation Mini-Platform for the Isolation, Enrichment, and Quantification of Biomarkers and Its Potential Use for Liquid Biopsy

Guzman¹,

Guzman

2020

Biomedicines

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Biomarker detection for disease diagnosis, prognosis, and therapeutic response is becoming increasingly reliable and accessible. Particularly, the identification of circulating cell-free chemical and biochemical substances, cellular and subcellular entities, and extracellular vesicles has demonstrated promising applications in understanding the physiologic and pathologic conditions of an individual. Traditionally, tissue biopsy has been the gold standard for the diagnosis of many diseases, especially cancer. More recently, liquid biopsy for biomarker detection has emerged as a non-invasive or minimally invasive and less costly method for diagnosis of both cancerous and non-cancerous diseases, while also offering information on the progression or improvement of disease. Unfortunately, the standardization of analytical methods to isolate and quantify circulating cells and extracellular vesicles, as well as their extracted biochemical constituents, is still cumbersome, time-consuming, and expensive. To address these limitations, we have developed a prototype of a portable, miniaturized instrument that uses immunoaffinity capillary electrophoresis (IACE) to isolate, concentrate, and analyze cell-free biomarkers and/or tissue or cell extracts present in biological fluids. Isolation and concentration of analytes is accomplished through binding to one or more biorecognition affinity ligands immobilized to a solid support, while separation and analysis are achieved by high-resolution capillary electrophoresis (CE) coupled to one or more detectors. When compared to other existing methods, the process of this affinity capture, enrichment, release, and separation of one or a panel of biomarkers can be carried out on-line with the advantages of being rapid, automated, and cost-effective. Additionally, it has the potential to demonstrate high analytical sensitivity, specificity, and selectivity. As the potential of liquid biopsy grows, so too does the demand for technical advances. In this review, we therefore discuss applications and limitations of liquid biopsy and hope to introduce the idea that our affinity capture-separation device could be used as a form of point-of-care (POC) diagnostic technology to isolate, concentrate, and analyze circulating cells, extracellular vesicles, and viruses.

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Section: The Potential Use Of Iace For Extracellular Vesicle Studimentioning

confidence: 99%

Section: The Potential Use Of Iace For Extracellular Vesicle Studimentioning

confidence: 99%

A Two-Dimensional Affinity Capture and Separation Mini-Platform for the Isolation, Enrichment, and Quantification of Biomarkers and Its Potential Use for Liquid Biopsy

Guzman¹,

Guzman

2020

Biomedicines

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“…While the ability to identify biomarkers at a single-cell resolution is certainly invaluable in the fight against infectious diseases, current flow cytometer systems are typically bulky and expensive, thereby limiting their use in a laboratory setting [100]. Fortunately, advancements in microfluidics and low-cost electronics have given rise to the development of portable platforms that can perform single-cell analysis in a point-of-care (POC) setting.…”

Section: Toward Point-of-care Applicationsmentioning

confidence: 99%

The Role of Single-Cell Technology in the Study and Control of Infectious Diseases

Lin

Tay

Lu³

et al. 2020

Cells

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The advent of single-cell research in the recent decade has allowed biological studies at an unprecedented resolution and scale. In particular, single-cell analysis techniques such as Next-Generation Sequencing (NGS) and Fluorescence-Activated Cell Sorting (FACS) have helped show substantial links between cellular heterogeneity and infectious disease progression. The extensive characterization of genomic and phenotypic biomarkers, in addition to host–pathogen interactions at the single-cell level, has resulted in the discovery of previously unknown infection mechanisms as well as potential treatment options. In this article, we review the various single-cell technologies and their applications in the ongoing fight against infectious diseases, as well as discuss the potential opportunities for future development.

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“…Since the introduction of the Coulter principle for particle counting [1], the interaction of single particles with electric fields has been exploited in several applications within life sciences, medicine, and environmental monitoring. In particular, in the last two decades, the development of microfabrication technologies has allowed the implementation of microfluidic impedance cytometers for the high-throughput label-free electrical characterization of blood cells, mammalian cell lines, microorganisms, and plant cells (see reviews) [2][3][4].…”

Section: Introductionmentioning

confidence: 99%

A neural network approach for real-time particle/cell characterization in microfluidic impedance cytometry

et al. 2020

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Microfluidic applications such as active particle sorting or selective enrichment require particle classification techniques that are capable of working in real time. In this paper, we explore the use of neural networks for fast label-free particle characterization during microfluidic impedance cytometry. A recurrent neural network is designed to process data from a novel impedance chip layout for enabling real-time multiparametric analysis of the measured impedance data streams. As demonstrated with both synthetic and experimental datasets, the trained network is able to characterize with good accuracy size, velocity, and crosssectional position of beads, red blood cells, and yeasts, with a unitary prediction time of 0.4 ms. The proposed approach can be extended to other device designs and cell types for electrical parameter extraction. This combination of microfluidic impedance cytometry and machine learning can serve as a stepping stone to real-time single-cell analysis and sorting.

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Cell Cytometry: Review and Perspective on Biotechnological Advances

Cited by 116 publications

References 129 publications

A Two-Dimensional Affinity Capture and Separation Mini-Platform for the Isolation, Enrichment, and Quantification of Biomarkers and Its Potential Use for Liquid Biopsy

A Two-Dimensional Affinity Capture and Separation Mini-Platform for the Isolation, Enrichment, and Quantification of Biomarkers and Its Potential Use for Liquid Biopsy

The Role of Single-Cell Technology in the Study and Control of Infectious Diseases

A neural network approach for real-time particle/cell characterization in microfluidic impedance cytometry

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