Cell cycle‐related and expression‐elevated protein in tumor overexpression is associated with proliferation behaviors and poor prognosis in non‐small‐cell lung cancer

Abstract

The cell cycle‐related and expression‐elevated protein in tumor (CREPT) is overexpressed in several human malignancies. However, the clinical relevance of CREPT expression and its biological role in non‐small‐cell lung cancer (NSCLC) remains unclear. In this study, we detected the expression of CREPT in both NSCLC tissues and cell lines by immunohistochemistry, Western blot analysis, and RT‐PCR. The correlation between CREPT expression and clinicopathologic features was analyzed in 271 NSCLC patients. The prognostic value of CREPT expression was evaluated by Kaplan–Meier analysis and Cox regression analysis. CREPT was overexpressed in Calu‐1 cell lines by using plasmid vector and its biological function was explored both in vitro and in vivo. We found that CREPT was significantly overexpressed in NSCLC compared with paired adjacent non‐tumor tissues, and the expression level of CREPT was correlated with tumor differentiation, lymph node metastasis, and clinical stage. Kaplan–Meier analysis showed that the recurrence‐free survival and overall survival of high CREPT expression groups were significantly shorter than those of the low CREPT expression group. Multivariate analysis identified that CREPT might be an independent biomarker for the prediction of NSCLC prognosis. Overexpression of CREPT increased cell proliferation and enhanced the migration and invasion ability of Calu‐1 cells (a human NSCLC cell line with relative low CRPET expression) in vitro. Moreover, CREPT overexpression promoted tumor growth in a nude mice model. These results suggest that CREPT is closely relevant to the proliferation of NSCLC cells and it might be a potential prognostic marker in NSCLC patients.

CREPT Promotes Melanoma Progression Through Accelerated Proliferation and Enhanced Migration by RhoA-Mediated Actin Filaments and Focal Adhesion Formation

“…Cell migration and metastases formation are crucial for malignant progression, which leads to increased mortality in cancer patients. Recent studies revealed the oncogenic potential of CREPT expression in multiple cancer types, including correlation with poor prognosis, induction of cell proliferation in vitro, and tumorigenesis in vivo [26][27][28]. Knockdown of RPRD1B increased cell sensitivity to treatment in endometrial adenocarcinoma, which suggests a therapeutic potential for targeting CREPT [21].…”

Histone deacetylase 9 downregulation decreases tumor growth and promotes apoptosis in non‐small cell lung cancer after melatonin treatment

“…The transwell migration and invasion assays were conducted using 6.5‐mm transwell insert chambers (Corning Costar Corp.) with or without matrix gel as reported previously . After 24 hours treatment with or without 2 mmol/L melatonin, the migrated or invaded cells were fixed, stained with 0.1% crystal violet, photographed, and counted.…”

CHCHD2 is a potential prognostic factor for NSCLC and is associated with HIF-1a expression

“…The expression of CHCHD2 and HIF-1α protein in all the tissue sections of the subjects were investigated by rabbit antibodies (anti-CHCHD2, diluted 1:50, ABGENT, San-Diego, CA or anti-HIF-1α, diluted 1:100, Proteintech™, Chicago, USA). The detailed operation process and staining score of immunohistochemistry accordance with our previous methods [18]. The final immunohistochemical staining score reported is the average of the scores from the tow investigators.…”