Cell cycle mechanisms of sister chromatid separation; Roles of Cut1/separin and Cut2/securin

Yanagida, Mitsuhiro

doi:10.1046/j.1365-2443.2000.00306.x

Cited by 103 publications

(68 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This complex is cleaved by separase that, in turn, is inhibited by securin. At the anaphase entry, the APCdependent degradation of securin enables separase activation and as a consequence cleavage of the cohesin complex, thereby allowing sister chromatid separation (Uhlmann et al, 1999(Uhlmann et al, , 2000Yanagida, 2000). Proteolysis of securin is ensured by APC Cdc20 before anaphase onset, but its degradation is maintained until the end of G1 by APC Cdh1 (Nasmyth, 2001;Zur and Brandeis, 2001).…”

Section: Apc Substrates Controlling Anaphase Entrymentioning

confidence: 99%

The anaphase-promoting complex: a key factor in the regulation of cell cycle

et al. 2005

View full text Add to dashboard Cite

Events controlling cell division are governed by the degradation of different regulatory proteins by the ubiquitin-dependent pathway. In this pathway, the attachment of a polyubiquitin chain to a substrate by an ubiquitin-ligase targets this substrate for degradation by the 26S proteasome. Two different ubiquitin ligases play an important role in the cell cycle: the SCF (Skp1/Cullin/ F-box) and the anaphase-promoting complex (APC). In this review, we describe the present knowledge about the APC. We pay particular attention to the latest results concerning APC structure, APC regulation and substrate recognition, and we discuss the implication of these findings in the understanding the APC function.

show abstract

Section: Apc Substrates Controlling Anaphase Entrymentioning

confidence: 99%

The anaphase-promoting complex: a key factor in the regulation of cell cycle

et al. 2005

View full text Add to dashboard Cite

show abstract

“…The spindle checkpoint requires the interaction of Mad2p with the anaphase-promoting complex (APC) 1 (7,8). APC is a multisubunit ubiquitin ligase, which targets the anaphase inhibitor securin and B-type cyclins for degradation, triggering chromosome segregation at the metaphase-anaphase transition (9,10). APC inhibition during checkpoint activation is likely to occur through a direct interaction of Mad2p with the Slp1p/Cdc20/Fizzy/p55CDC protein (7,8).…”

mentioning

confidence: 99%

DNA Replication Checkpoint Control Mediated by the Spindle Checkpoint Protein Mad2p in Fission Yeast

Sugimoto

Murakami

Tonami

et al. 2004

Journal of Biological Chemistry

View full text Add to dashboard Cite

The relationship between the DNA replication and spindle checkpoints of the cell cycle is unclear, given that in most eukaryotes, spindle formation occurs only after DNA replication is complete. Fission yeast rad3 mutant cells, which are deficient in DNA replication checkpoint function, enter, progress through, and exit mitosis even when DNA replication is blocked. In contrast, the entry of cds1 mutant cells into mitosis is delayed by several hours when DNA replication is inhibited. We show here that this delay in mitotic entry in cds1 cells is due in part to activation of the spindle checkpoint protein Mad2p. In the presence of the DNA replication inhibitor hydroxyurea (HU), cds1 mad2 cells entered and progressed through mitosis earlier than did cds1 cells. Overexpression of Mad2p or inactivation of Slp1p, a regulator of the anaphase-promoting complex, also rescued the checkpoint defect of HU-treated rad3 cells. Rad3p was shown to be involved in the physical interaction between Mad2p and Slp1p in the presence of HU. These results suggested that Mad2p and Slp1p act downstream of Rad3p in the DNA replication checkpoint and that Mad2p is required for the DNA replication checkpoint when Cds1p is compromised.

show abstract

“…The cohesin complex is composed of at least four proteins, Scc1\ Mcd1, Scc3, Smc1 and Smc3, in budding yeast [180][181][182][183]. Similar proteins and their regulators exist in fission yeast and other eukaryotes, indicating that this mechanism is conserved [184,185]. Loss of any of these proteins brings about premature chromosome separation even if the APC is inactivated.…”

Section: Cohesin In Meiosismentioning

confidence: 99%

DNA replication and damage checkpoints and meiotic cell cycle controls in the fission and budding yeasts

Murakami¹,

Nurse

2000

Biochemical Journal

View full text Add to dashboard Cite

The cell cycle checkpoint mechanisms ensure the order of cell cycle events to preserve genomic integrity. Among these, the DNA-replication and DNA-damage checkpoints prevent chromosome segregation when DNA replication is inhibited or DNA is damaged. Recent studies have identified an outline of the regulatory networks for both of these controls, which apparently operate in all eukaryotes. In addition, it appears that these checkpoints have two arrest points, one is just before entry into mitosis and the other is prior to chromosome separation. The former point requires the central cell-cycle regulator Cdc2 kinase, whereas the latter involves several key regulators and substrates of the ubiquitin ligase called the anaphase promoting complex. Linkages between these cell-cycle regulators and several key INTRODUCTIONIn eukaryotes the cell cycle is divided into two phases, interphase and mitosis [1]. Interphase consists of G1, S and G2 phases. Mitosis can be sub-divided into prophase, metaphase, anaphase and telophase. Chromosomes condense during prophase, align during metaphase, separate during anaphase and decondense during telophase. There are several control points during the cell cycle : in late G1, called Start in yeast or the Restriction point in mammals, in late G2 and just prior to anaphase. To pass each point, cells have to fulfil several prerequisites. Before passing Start, cells can undergo two developmental programmes, i.e. entry into the mitotic cell cycle or sexual development. Adequate nutritional conditions and a critical cell size are required to traverse Start. During G2, cells have to check whether DNA replication is completed and ensure that DNA is not damaged. Before chromosome separation cells also examine whether chromosomes are aligned and spindles are formed properly. These cell-cycle checkpoints are the mechanisms that govern the order of the cell-cycle events, because if the order of the events is incorrect then a full complement of genetic information is not transmitted at cell division, which may lead to cancer in higher eukaryotes.Much is now known about regulation of the eukaryotic cell cycle but two areas have yet to be fully understood. The first area is concerned with the molecular mechanisms acting during the DNA-replication and DNA-damage checkpoints, which block mitosis if DNA replication is incomplete or DNA is damaged. The second concerns the controls which operate during the meiotic cell cycle. In recent years, some progress has been made in both of these areas and the purpose of the present review is to Abbreviations used : APC, anaphase-promoting complex ; Cln, G1 cyclin ; FHA, forkhead associated ; PCNA, proliferating-cell nuclear antigen ; SPB, spindle-pole body ; CDK, cyclin-dependent kinase ; RFC, replication factor C ; MCM, minichromosome maintenance ; ORC, origin-recognition complex.1 To whom correspondence should be addressed (e-mail murakami!icrf.icnet.uk). REGULATION OF MITOTIC CELL CYCLE BY CYCLIN-DEPENDENT KINASE (CDK) IN FISSION YEASTThere are a number of protein...

show abstract

Cell cycle mechanisms of sister chromatid separation; Roles of Cut1/separin and Cut2/securin

Cited by 103 publications

References 31 publications

The anaphase-promoting complex: a key factor in the regulation of cell cycle

The anaphase-promoting complex: a key factor in the regulation of cell cycle

DNA Replication Checkpoint Control Mediated by the Spindle Checkpoint Protein Mad2p in Fission Yeast

DNA replication and damage checkpoints and meiotic cell cycle controls in the fission and budding yeasts

Contact Info

Product

Resources

About