Cell Cycle Inhibitory Protein p27 in Human Middle Ear Cholesteatoma

Kuczkowski, Jerzy; Bakowska, Alicja; Pawełczyk, Tadeusz; Narożny, Waldemar; Mikaszewski, Bogusław

doi:10.1159/000094377

Cited by 4 publications

(7 citation statements)

References 22 publications

(15 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, increased p63 expression and keratinocyte markers demonstrate uncoordinated hyperproliferation, migration and invasion properties 25 – 28 . The cell cycle inhibitory protein p27 is down-regulated, 29 and the ErbB-2 protein for accelerated cell proliferation and apoptosis is over-expressed 30 . The c-jun protein (associated with proliferation), c-myc protein (associated with differentiation) and p53 tumour suppressor gene (associated with apoptosis) are all up-regulated in cholesteatoma 2 , 31 , 32 .…”

Section: Molecular Overviewmentioning

confidence: 99%

Acquired cholesteatoma: summary of the cascade of molecular events

Louw

2013

J. Laryngol. Otol.

View full text Add to dashboard Cite

show abstract

Section: Molecular Overviewmentioning

confidence: 99%

Acquired cholesteatoma: summary of the cascade of molecular events

Louw

2013

J. Laryngol. Otol.

View full text Add to dashboard Cite

show abstract

“…An upregulation of epidermal growth factor (EGF) and its receptor (EGFR), and of keratinocyte growth factor (KGF) and its receptor (KGFR) have previously been reported in cholesteatomas (3)(4)(5)(6). Further studies have suggested that an upregulation of these growth factors and their receptors induces cell proliferation of keratinocytes in cholesteatomas (7)(8)(9). In addition to growth factors, the possible roles of microRNAs (miRNA) in the formation of cholesteatomas have recently been proposed (10,11).…”

Section: Introductionmentioning

confidence: 96%

MicroRNA let-7a suppresses the growth and invasion of cholesteatoma keratinocytes

Zhang

Chen

Qin

2014

Molecular Medicine Reports

View full text Add to dashboard Cite

Cholesteatomas are benign epidermally‑derived lesions of the temporal bone that are caused by migration of hyperproliferative keratinocytes into the middle ear and mastoid cavity. The molecular mechanisms that regulate the pathogenesis of cholesteatomas are currently not fully understood. The present study demonstrated the antigrowth and anti‑invasive effects of let‑7a microRNA (miRNA) on cholesteatoma keratinocytes. Let‑7a inhibited the growth of cholesteatoma keratinocytes through two different mechanisms: Restriction of the proliferation of keratinocytes by promoting cell cycle arrest in the G0/G1 phase, and the induction of apoptosis of the cells. In addition to its role in the inhibition of cell growth, let‑7a suppressed the migration and invasion of cholesteatoma keratinocytes. A mechanistic study showed that let‑7a downregulated the expression of miR‑21. Considering the function of miR‑21 in the regulation of proliferation and apoptosis, let‑7a may control cell proliferation and apoptosis by regulating miR‑21, and its targets, in cholesteatoma keratinocytes. In conclusion, the present study showed that let‑7a downregulates the expression of miR‑21, resulting in the suppression of proliferation and induction of apoptosis. The results of the present study reveal the crucial role of let‑7a miRNA in the inhibition of growth and invasion of cholesteatoma keratinocytes.

show abstract

“…P27 is the novel cyclin-dependent kinase inhibitor that acts as a tumor suppressor gene, arrests the cell cycle in phase G1, and stops cellular proliferation. 17 , 18 A limited number of studies have focused on the effect of p27 on the cholesteatoma pathogenesis with conflicting results. 16 - 18 Only one study 18 investigated the role of p27 on recurrence of the cholesteatoma without matching the results with the skin tissue of the same patient.…”

Section: Introductionmentioning

confidence: 99%

“… 17 , 18 A limited number of studies have focused on the effect of p27 on the cholesteatoma pathogenesis with conflicting results. 16 - 18 Only one study 18 investigated the role of p27 on recurrence of the cholesteatoma without matching the results with the skin tissue of the same patient. Additionally, to the best of the authors’ knowledge, the role of p27 on extensiveness and bone erosion degree of the cholesteatoma has not been evaluated, yet.…”

Section: Introductionmentioning

confidence: 99%

Differential Expression of Ki-67 and P27 in Cholesteatoma Compared to Skin Tissue Predicts the Prognosis of Adult Acquired Cholesteatoma

et al. 2021

View full text Add to dashboard Cite

Background : The aim of this study was to compare the differential Ki-67 and p27 staining properties of acquired cholesteatoma in adult patients for prognostic analysis. Methods: Forty-two adult patients with acquired cholesteatoma were enrolled. The cholesteatoma and matched meatal skin tissues of the patients were immunostained with Ki-67 and p27 antibodies. Canal wall down mastoidectomy was performed in all patients. The differential staining properties––positive staining in the cholesteatoma and negative staining in the skin tissue (C+S-), negative staining in the cholesteatoma and positive staining in the skin tissue(C-S+)––were compared for bone erosion scores (BES), stage, and recurrence rates. Results: Isolated findings in the cholesteatoma tissues, without matching with the skin tissues, demonstrated that stage and recurrence rates were not related to findings in the cholesteatoma tissues ( P ˃ .05). However, C+S- for Ki-67 and C-S+ for p27 are risk factors for worse prognosis including advanced stage ( P < .001 for Ki-67 and P = .008 for p27), BES values ( P < .001 for Ki-67 and P = .001 for p27), and recurrence rates ( P < .001 for Ki-67 and P = .037 for p27). Conclusion: This is the first paper assessing the cholesteatoma prognosis according to the differential Ki-67 and p27 staining properties of cholesteatoma and healthy skin tissues. Cellular proliferation rate in the cholesteatoma is important but insufficient by itself for predicting the prognosis of cholesteatoma patients. Patients having lower basal levels of cellular proliferation rate and higher cellular activity in the cholesteatoma tissue are prone to worse prognosis with increased stage, recurrence rates, and degree of bone erosion.

show abstract

Cell Cycle Inhibitory Protein p27 in Human Middle Ear Cholesteatoma

Cited by 4 publications

References 22 publications

Acquired cholesteatoma: summary of the cascade of molecular events

Acquired cholesteatoma: summary of the cascade of molecular events

MicroRNA let-7a suppresses the growth and invasion of cholesteatoma keratinocytes

Differential Expression of Ki-67 and P27 in Cholesteatoma Compared to Skin Tissue Predicts the Prognosis of Adult Acquired Cholesteatoma

Contact Info

Product

Resources

About