Cell cycle dependent regulation of gap junction coupling and apoptosis in GFSHR-17 granulosa cells

Schlie, Sabrina; Mazur, Karolina; Bintig, Willem; Ngezahayo, Anaclet

doi:10.4236/jbise.2010.39118

Cited by 9 publications

(4 citation statements)

References 22 publications

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“…There are published data that the effect of AuNPs is based on G0/G1 phase accumulation, S and G2/M phase depletion, as well as on reduced ATP levels in human oral squamous carcinoma cells (HSC-3) [ 48 ]. Cell cycle regulation may be addressed by violation of focal contacts of cells with substrate and cell transfer to suspended fraction in 2D culture and inhibition cell-to-cell contacts in gap junction in 3D culture [ 48 – 50 ]. Due to nanosize of AuNPs (near 15 nm), it cannot be centers of cohesion for cells.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Study of Influence of Au Nanoparticles on HT29 and SPEV Cell Lines

et al. 2017

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Cell culture models are excellent tools for potential toxicity of nanoparticles and fundamental investigations in cancer research. Thus, information about AuNP potential toxicity and effects on human health is necessary for the use of nanomaterials in clinical settings. The aim of our research is to examine the effects of AuNPs on the epithelial origin cell lines: continuous and oncogenic. Embryonic porcine kidney epithelial inoculated (SPEV) cell line and colorectal carcinoma cell line (HT29) were used. In the test cultures, the cell proliferation, necrosis/apoptosis, and multicellular spheroids generation were evaluated. We demonstrated that AuNP concentrations of 6–12 μg/ml reduced the proliferation of SPEV and HT29 cells and increased the cell number at early and late stages of apoptosis and necrosis. It was shown that small concentrations of AuNPs (1–3 μg/ml) stimulate multicellular spheroid formation by HT29 and SPEV cells. However, higher AuNP concentrations (6–12 μg/ml) had both cytotoxic and anti-cohesive effects on cell in suspension. The large sensitiveness to the action of AuNPs was shown by the line of HT29 (6 μg/ml) as compared to the SPEV cells (12 μg/ml). This experimental study of the effect of AuNPs on SPEV and HT29 cell lines will justify their further application in AuNP-mediated anticancer treatment.

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Section: Discussionmentioning

confidence: 99%

In Vitro Study of Influence of Au Nanoparticles on HT29 and SPEV Cell Lines

et al. 2017

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“…They allow intercellular communication by chemical signals passing through these cell-cell channels [40]. They are known to play a fundamental role in differentiation, cell cycle progression and cell survival [41,42].…”

Section: Printing Three-dimensional Ex Vivo Cell Models and Tissuementioning

confidence: 99%

Laser-based micro- and nanofabrication for applications in biomedicine

Koch

Sajti

Schwenke

et al. 2012

AIP Conference Proceedings

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Applications of laser-based ultrasonics to the characterization of the internal structure of teeth J. Acoust. Soc. Am. 114, 542 (2003); 10.1121/1.1578080Laser-based surface acoustic wave spectrometer for industrial applications Rev. Sci. Instrum. 74, 667 (2003); 10.1063/1.1512680Laser-based processing of polymer nanocomposites for chemical sensing applications Abstract. Lasers are increasingly used to generate functional implants. This includes surface structuring to promote or avoid cell adhesion, laser sintering of metals to yield specific mechanical strength and resilience, and laser-generation of microstructured scaffolds. In this study, two further laser-based techniques for fabrication of multifunctional materials and implants are presented. First, a laser-based method for printing living cells is described. This technique allows to cover implant surfaces with a patient-identic biological footprint or to generate tissue constructs with or without scaffolds. Second, a novel laser-based technique is presented for controlled synthesis of multifunctionalized nanoparticles and nanoparticleembedded composite implants. It is shown that beside cell-targeting, additional functions can be rigorously combined on a nanoparticle surface allowing complex cellular manipulations. Finally, using as a model implant a thermoplastic catheter, embedded nanoparticles reveal antibacterial and cellular proliferative behaviors by changing the nature of composite materials, opening new strategies for laser-generated implants.

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“…Adherens junctions are fundamental for tissue morphogenesis and cohesion; they consist mainly of cadherins (calciumdependent adherent proteins). Gap junctions are cell-cell channels that allow intercellular communication by passing chemical signals; they consist of connexins (Richard 2000) and are known to have a fundamental role in differentiation, cell cycle progression, and cell survival (Schlie et al 2010).…”

Section: Printed Skin Tissuementioning

confidence: 99%