Cell cycle-dependent expression of nuclear matrix proteins of ehrlich ascites cells studied by in vitro translation

Bludau, H.; Kopun, Marijana; Werner, Dieter

doi:10.1016/0014-4827(86)90551-3

Cited by 14 publications

(5 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This arrangement of transcription elements has been suggested to play a role in the activation of DNA replication in several viral (6,20,33) and cellular (36) systems. Several lines of evidence point to a cell cycledependent regulation of lamin expression; in particular, B-type lamins appear to be synthesized only in S-phase (3,9). Our data are in accord with this coupling for a human gene.…”

Section: Discussionsupporting

confidence: 87%

“…Several reports indicate that lamin B proteins are preferentially synthesized during S-phase (3,9), in accord with the notion that the nuclear lamina may be related to changes in the dynamics of the nuclear envelope or chromatin (12). Our findings tend to support the model relating early replication to actively transcribed genes.…”

supporting

confidence: 90%

“…In fact, the stability of lamin mRNAs could be an important aspect of cell cycle-dependent regulation. Lamin B proteins, for example, are known to be expressed mainly in S-phase (3,9); as a consequence, their RNAs could be rapidly degraded after this phase. In this regard, our finding of a second lamin B-type cDNA that does not contain the three A+T-rich boxes points to alternative polyadenylation as a possible regulatory mechanism.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

The gene for a novel human lamin maps at a highly transcribed locus of chromosome 19 which replicates at the onset of S-phase.

Biamonti¹,

Giacca²,

Perini³

et al. 1992

Mol. Cell. Biol.

133

111

View full text Add to dashboard Cite

A previously described human DNA fragment which is replicated early in S-phase of HL-60 cell DNA (C. (7,19). Elucidation of the underlying mechanisms will constitute a major step in understanding the nuclear dynamics of the process, cell growth, and differentiation.We have previously described the isolation and cloning of a human DNA fragment (pB48; 1,560 bp) that belongs to the fraction of DNA replicated at the onset of S-phase in synchronized HL-60 cells (35). Analysis of this fragment indicated that it derives from an expressed region of the human genome, since in Northern (RNA) blot experiments, it hybridized to two mRNAs; moreover, it contains a binding site for the transcription factor USF/MLTF and an active promoter overlapping a putative CpG island (8,13). This finding is consistent with the notion that actively transcribed regions of the human genome are preferentially replicated early in S-phase (7,14,15,19,36). In this context, elucidation of the structural features of an early-replicating DNA and identification of the encoded genes could help us to understand the biological significance of this phenomenon. pB48 DNA represents an opportunity to explore this approach. In this work, we have extended our studies to a chromosomal region of 13.7 kb encompassing pB48, which was mapped by in situ hybridization to the subtelomeric G-negative band p13.3 of chromosome 19. The 13.7-kb region was found to code for several transcripts and to contain at least two closely spaced and nonoverlapping genes, one of which corresponded to a B-type nuclear lamin. Lamin proteins line the inner side of the nuclear envelope * Corresponding author.with a meshwork of filamentous structures and are thought to play a role in nuclear stability, chromatin structure, and gene expression (12,23,29). Several reports indicate that lamin B proteins are preferentially synthesized during S-phase (3, 9), in accord with the notion that the nuclear lamina may be related to changes in the dynamics of the nuclear envelope or chromatin (12). Our findings tend to support the model relating early replication to actively transcribed genes. MATERIALS AND METHODSCells, cell lines, and probes. Peripheral blood lymphocytes were obtained from healthy donors and prepared according to the Ficoll-metrizoate method as described elsewhere (10). Lymphocytes were grown in RPMI 1640 (GIBCO), 10% fetal calf serum, 50 ,g of gentamicin per ml, 2 mM L-glutamine, and 50 p,g of phytohemagglutinin per ml. Human primary fibroblasts were cultured in Dulbecco modified Eagle medium (DMEM; GIBCO) supplemented with 15% fetal calf serum, 50 jig of gentamicin per ml, and 2 mM L-glutamine.To obtain resting cells, confluent fibroblasts were grown for 7 days in DMEM supplemented with 0.25% fetal calf serum. Human epidermoid carcinoma A-431 cells were obtained from the American Type Culture Collection and maintained in DMEM (GIBCO) supplemented with 10% fetal calf serum.The rDNA probe (plasmid LS6BB) (32) was kindly provided by G. N. Ranzani. The human 3-globin DNA probe was pr...

show abstract

Section: Discussionsupporting

confidence: 87%

supporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The gene for a novel human lamin maps at a highly transcribed locus of chromosome 19 which replicates at the onset of S-phase.

Biamonti¹,

Giacca²,

Perini³

et al. 1992

Mol. Cell. Biol.

133

111

View full text Add to dashboard Cite

show abstract

“…with a meshwork of filamentous structures and are thought to play a role in nuclear stability, chromatin structure, and gene expression (12,23,29). Several reports indicate that lamin B proteins are preferentially synthesized during S-phase (3,9), in accord with the notion that the nuclear lamina may be related to changes in the dynamics of the nuclear envelope or chromatin (12). Our findings tend to support the model relating early replication to actively transcribed genes.…”

supporting

confidence: 85%

The Gene for a Novel Human Lamin Maps at a Highly Transcribed Locus of Chromosome 19 which Replicates at the Onset of S-Phase

Biamonti

Giacca

Perini

et al. 1992

Molecular and Cellular Biology

View full text Add to dashboard Cite

A previously described human DNA fragment which is replicated early in S-phase of HL-60 cell DNA (C. Tribioli, G. Biamonti, M. Giacca, M. Colonna, S. Riva, and A. Falaschi, Nucleic Acids Res. 15:10211-10232, 1987) was used to screen a genomic library in lambda Ch28. A clone which contained a 13.7-kb insert (L30E) found to code for several transcripts was isolated. The transcription of L30E DNA exhibited a complex pattern and a tissue-specific and proliferation-dependent type of regulation. The data were consistent with two tandemly arranged transcription units, the 3' end of one separated from the 5' end of the other by a sequence of about 600 bp containing an active promoter. The isolation and sequencing of L30E-specific cDNAs permitted identification of two genes, one of which encoded a B-type human lamin (analogous to mouse lamin B2). L30E DNA was mapped by in situ hybridization at the G-negative subtelomeric band p13.3 of chromosome 19. Interestingly, in synchronized HL-60 cells, L30E DNA is replicated in the first minute of S-phase. Replication of the lamin gene early in S-phase may reflect a coupling between early replication and transcription of genes for S-phase-specific proteins such as lamins.

show abstract

“…Biochemical experiments revealed that lamins are synthesized throughout the interphase of cultured CHO cells (Gerace et al, 1984), and that in vitro translatable lamin mRNAs are predominantly detectable in Ehrlich ascites cells at S-phase (Bludau et al, 1986). These data suggest that nuclear envelope growth and lamin synthesis are coordinated during the cell cycle.…”

Section: Discussionmentioning

confidence: 82%

The lamin CxxM motif promotes nuclear membrane growth

Prüfert

Vogel

Krohne

2004

Journal of Cell Science

103

View full text Add to dashboard Cite

We analyzed the influence of lamins on nuclear envelope growth in cultured Xenopus A6 cells by the overexpression of human lamin A, Xenopus and zebrafish lamins B2 and Drosophila lamins Dm0 and C as GFP fusion proteins. Lamins containing a CxxM motif in their primary sequence (lamins A, B2, Dm0) induced the formation of lobulated nuclei with multi-membrane-layered, highly folded nuclear membranes and intranuclear membrane assemblies, as observed by electron microscopy. Such morphological alterations were not observed with Drosophila lamin C, a lamin without this motif or with a lamin B2 mutant (B2-SxxM) where the cysteine of the CxxM motif is replaced by a serine. Drosophila lamin C mutants containing a CxxM motif behaved like B-type lamins thus confirming that this tetrapeptide is directly involved in the morphological changes we observed. Nuclear membrane proliferation could also be induced by lamin B2 in COS-7 cells and in zebrafish embryos but not by human lamin A in COS-7 cells. We speculate that the human lamin A is incompletely processed in Xenopus A6 cells and therefore behaves in this cell line like a B-type lamin. Our results indicate that the CxxM motif of B-type lamins has a dual function: it mediates lamin targeting to the inner nuclear membrane thereby promoting nuclear membrane growth.

show abstract

Cell cycle-dependent expression of nuclear matrix proteins of ehrlich ascites cells studied by in vitro translation

Cited by 14 publications

References 20 publications

The gene for a novel human lamin maps at a highly transcribed locus of chromosome 19 which replicates at the onset of S-phase.

The gene for a novel human lamin maps at a highly transcribed locus of chromosome 19 which replicates at the onset of S-phase.

The Gene for a Novel Human Lamin Maps at a Highly Transcribed Locus of Chromosome 19 which Replicates at the Onset of S-Phase

The lamin CxxM motif promotes nuclear membrane growth

Contact Info

Product

Resources

About