Cell cycle dependence for activation of Epstein-Barr virus by inhibitors of protein synthesis or medium deficient in arginine

The Regulated Expression of Epstein--Barr Virus. III. Proteins Specified by EBV During the Lytic Cycle

“…Synchronization of the cells before CH treatment allows the synthesis of EA in uninfected Raji cells. These observations are identical to those reported by Hampar et al (1976) who showed that addition of CH to Raji cells at the beginning of the S phase permitted the synthesis of EA 5 h after the removal of the inhibitor. The observation that the primary proteins appear in subgroups leads to the hypothesis that a sequence of events, specified by EBV, occurs immediately after infection and is essential for subsequent synthesis of EA.…”

The Spontaneous and Induced Synthesis of Epstein--Barr Virus Antigens in Raji Cells Immobilized on Surfaces Coated with Anti-lymphocyte Globulin

“…The failure of the cells to express EA when seeded at high cell densities may indicate that cell growth is required for activation of EBV. This conclusion is supported by the fact that treatment of Raji cells in S phase with IdUrd and other substances induced EA much more efficiently and after a shorter time than treatment of unsynchronized cells (Hampar et al, 1976).…”

“…At high concentrationg of ALG the cells grew slowly and the cultures tended to have lower viabilities, while low concentrations of ALG which did not affect the viability of cells seemed to stimulate cell growth. Treatment of cells with IdUrd is known to induce EA expression (Hampar et al, 1976). Therefore, we tested the ability of ALG-treated Raji cells to enter into EA synthesis after IdUrd treatment.…”

Induction of Epstein-Barr Virus Early Antigen, Virus Capsid Antigen and Virus DNA Synthesis by Mitomycin C

“…For example, a number of metabolic inhibitors have been shown to induce cells to enter the productive EBV cycle. Iododeoxyuridine (IdUrd), bromodeoxyuridine (BrdUrd) and cycloheximide (CH) all have the capacity to induce this virus cycle (Gerber, 1972;Hampar et al, 1972aHampar et al, , 1976. In a number of other DNA virus-host cell systems mitomycin C (MMC) has also been shown to induce a virus lytic cycle (Rakusanova et al, 1976;Fogel, 1972;Kaplan et al, 1975).…”