Cell cycle control by the nucleo-cytoplasmic ratio in early Drosophila development

Edgar, Bruce A.; Kiehle, Caroline P.; Schubiger, Gerold

doi:10.1016/0092-8674(86)90771-3

Cited by 325 publications

(270 citation statements)

References 23 publications

Supporting

Mentioning

257

Contrasting

Unclassified

Order By: Relevance

“…Quiescence could therefore be triggered when a neuroblast's volume drops below a critical point. It has been shown that nucleocytoplasmic ratio plays an important role in controlling early embryonic cell division (Edgar et al 1986) and gene expression (Yasuda et al 1991). In agreement with this are experiments in grasshopper, in which the mitotic spindle is mechanically shifted during the anaphase of neuroblast divisions.…”

Section: The Postembryonic Phase Of Neuroblast Proliferationsupporting

confidence: 74%

Insights into neural stem cell biology from flies

Egger

Chell

Brand

2007

Phil. Trans. R. Soc. B

134

126

View full text Add to dashboard Cite

Drosophila neuroblasts are similar to mammalian neural stem cells in their ability to self-renew and to produce many different types of neurons and glial cells. In the past two decades, great advances have been made in understanding the molecular mechanisms underlying embryonic neuroblast formation, the establishment of cell polarity and the temporal regulation of cell fate. It is now a challenge to connect, at the molecular level, the different cell biological events underlying the transition from neural stem cell maintenance to differentiation. Progress has also been made in understanding the later stages of development, when neuroblasts become mitotically inactive, or quiescent, and are then reactivated postembryonically to generate the neurons that make up the adult nervous system. The ability to manipulate the steps leading from quiescence to proliferation and from proliferation to differentiation will have a major impact on the treatment of neurological injury and neurodegenerative disease.

show abstract

Section: The Postembryonic Phase Of Neuroblast Proliferationsupporting

confidence: 74%

Insights into neural stem cell biology from flies

Egger

Chell

Brand

2007

Phil. Trans. R. Soc. B

134

126

View full text Add to dashboard Cite

show abstract

“…Mats is required for proper chromosomal segregation: Up to the midblastula transition, the cellular function of Drosophila embryos relies on maternally loaded transcripts and protein products (Edgar et al 1986). Therefore, in maternally mats null embryos, the cell divisions up to the 16th cycle occur in the total absence of Mats function.…”

Section: Resultsmentioning

confidence: 99%

The mob as tumor suppressor Gene Is Essential for Early Development and Regulates Tissue Growth in Drosophila

Shimizu¹,

Ho²,

Lai

2008

Genetics

View full text Add to dashboard Cite

Studies in Drosophila have defined a new growth inhibitory pathway mediated by Fat (Ft), Merlin (Mer), Expanded (Ex), Hippo (Hpo), Salvador (Sav)/Shar-pei, Warts (Wts)/Large tumor suppressor (Lats), and Mob as tumor suppressor (Mats), which are all evolutionarily conserved in vertebrate animals. We previously found that the Mob family protein Mats functions as a coactivator of Wts kinase. Here we show that mats is essential for early development and is required for proper chromosomal segregation in developing embryos. Mats is expressed at low levels ubiquitously, which is consistent with the role of Mats as a general growth regulator. Like mammalian Mats, Drosophila Mats colocalizes with Wts/Lats kinase and cyclin E proteins at the centrosome. This raises the possibility that Mats may function together with Wts/Lats to regulate cyclin E activity in the centrosome for mitotic control. While Hpo/Wts signaling has been implicated in the control of cyclin E and diap1 expression, we found that it also modulates the expression of cyclin A and cyclin B. Although mats depletion leads to aberrant mitoses, this does not seem to be due to compromised mitotic spindle checkpoint function.

show abstract

“…Early on, cell division was observed to depend on the proportion of nuclear volume to cytoplasmic volume (the N/C ratio) (Prescott, 1956;Mitchison, 1971). As well, in metazoan embryonic development, the mid-blastula transition, which marks the initiation of zygotic gene transcription and the end of the embryo's dependency on maternal mRNA, occurs when the N/C ratio reaches a critical value (Edgar et al, 1986;Masui and Wang, 1998). In addition, an adult cell maintains a close relation not only between the size of the nucleus and the cytoplasmic volume but also in proportionality between cytoplasmic volume and the surface area of the nucleus (Swanson et al, 1991).…”

Section: Discussionmentioning

confidence: 99%

Migratory Localization of Cyclin D2-Cdk4 Complex Suggests a Spatial Regulation of the G1-S Transition

Wang

Xie

Zhang

et al. 2008

Cell Struct. Funct.

View full text Add to dashboard Cite

ABSTRACT. The association of the cyclin D-Cdk (DC) complex with retinoblastoma protein (pRb) is required for the G1-S transition of the cell cycle. Cyclin synthesis, nuclear localization and degradation are control mechanisms for the transition, but regulation of the DC complex nuclear import also contributes to the transition. Analysis of the timing of the G1-S transition in mammalian cell lines revealed acceleration with overexpression of cyclin D2 and Cdk4. Immunolocalization assays revealed that cyclin D2 and Cdk4 formed a complex in the cytoplasm and approached the nucleus. They accumulated on the cytosolic surfaces of the nuclear pores and then were arrested at the nuclear membrane before the nucleus reached a critical size. Finally, the complex was released into the nucleus and colocalized with pRb there, which led to pRb phosphorylation and DNA synthesis. The translocalization depended on the G1-S transition. In contrast, a truncated cyclin D2 that was not able to fully associate with Cdk4 lost the ability for release into the nucleus. This pattern of translocalization suggests a spatial separation of the cyclin D-Cdk complex from pRb and DNA in the nucleus to regulate the G1-S transition.

show abstract

Cell cycle control by the nucleo-cytoplasmic ratio in early Drosophila development

Cited by 325 publications

References 23 publications

Insights into neural stem cell biology from flies

Insights into neural stem cell biology from flies

The mob as tumor suppressor Gene Is Essential for Early Development and Regulates Tissue Growth in Drosophila

Migratory Localization of Cyclin D2-Cdk4 Complex Suggests a Spatial Regulation of the G1-S Transition

Contact Info

Product

Resources

About