Cell cycle and transmembrane mitochondrial potential analysis after treatment with chromium(<scp>iii</scp>), iron(<scp>iii</scp>), molybdenum(<scp>iii</scp>) or nickel(<scp>ii</scp>) and their mixtures

Terpiłowska, Sylwia; Siwicki, Andrzej K.

doi:10.1039/c8tx00233a

Cited by 17 publications

(6 citation statements)

References 27 publications

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“…88 They studied the impact of these metals on the cell cycle and mitochondrial transmembrane potential in a human hepatocyte model (HepG2) and published that these metals appeared to induce apoptosis and a loss of mitochondrial transmembrane potential. 89 Zarei et al also showed an association between NiCl 2 cytotoxicity and an increase in the ROS formation and glutathione depletion. 63 Moreover, interactions among heavy metal exposure, ROS, and signal transduction have been reported.…”

Section: ■ Discussionmentioning

confidence: 93%

“…Similarly, Terpilowska et al also showed that Cr and Ni chlorides may generate ROS and may disturb superoxide dismutase, catalase, and glutathione peroxidase . They studied the impact of these metals on the cell cycle and mitochondrial transmembrane potential in a human hepatocyte model (HepG2) and published that these metals appeared to induce apoptosis and a loss of mitochondrial transmembrane potential . Zarei et al also showed an association between NiCl 2 cytotoxicity and an increase in the ROS formation and glutathione depletion …”

Section: Discussionmentioning

confidence: 96%

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Toxicity and Metabolomic Impact of Cobalt, Chromium, and Nickel Exposure on HepaRG Hepatocytes

Bellouard

Gasser

Lenglet

et al. 2022

Chem. Res. Toxicol.

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Cobalt, chromium, and nickel are used in orthopedic prostheses. They can be released, accumulate in many organs, and be toxic. The aim of this study is to evaluate the cytotoxicity of these metals on human hepatocytes and to improve our knowledge of their cellular toxicity mechanisms by metabolomic analysis. HepaRG cells were incubated for 48 h with increasing concentrations of metals to determine their IC50. Then, a nontargeted metabolomic study using liquid chromatography–high-resolution mass spectrometry (LC–HRMS) was done at IC50 and at a lower concentration (100 nM), near to those found in the blood and liver of patients with prostheses. IC50 were defined at 940, 2, and 1380 μM for Co, Cr, and Ni, respectively. In vitro, Cr appears to be much more toxic than Co and Ni. Metabolomic analysis revealed the disruption of metabolic pathways from the low concentration of 100 nM, in particular tryptophan metabolism and lipid metabolism illustrated by an increase in phenylacetylglycine, a marker of phospholipidosis, for all three metals. They also appear to be responsible for oxidative stress. Dysregulation of these pathways impacts hepatocyte metabolism and may result in hepatotoxicity. Further investigations on accessible biological matrices should be conducted to correlate our in vitro results with the clinical data of prostheses-bearing patients.

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Section: ■ Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 96%

Toxicity and Metabolomic Impact of Cobalt, Chromium, and Nickel Exposure on HepaRG Hepatocytes

Bellouard

Gasser

Lenglet

et al. 2022

Chem. Res. Toxicol.

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“…Iron is an essential element for microorganism growth [36]; Fe 2+ oxidizes Fe 3+ to form stable iron oxide complexes in the presence of oxygen and water. These molecules are sequestered by siderophores; they are produced by bacteria and are defined as small peptide molecules that contain lateral chains and functional groups that can provide a set of high-affinity to coordinate iron ions [37].…”

Section: Siderophoresmentioning

confidence: 99%

Biocontrol of Postharvest Fruit Fungal Diseases by Bacterial Antagonists: A Review

et al. 2019

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This review deals with the main mechanisms of action exerted by antagonistic bacteria, such as competition for space and nutrients, suppression via siderophores, hydrolytic enzymes, antibiosis, biofilm formation, and induction of plant resistance. These mechanisms inhibit phytopathogen growth that affects postharvest fruit since quality and safety parameters are influenced by the action of these microorganisms, which cause production losses in more than 50% of fruit tree species. The use of synthetic fungicide products has been the dominant control strategy for diseases caused by fungi. However, their excessive and inappropriate use in intensive agriculture has brought about problems that have led to environmental contamination, considerable residues in agricultural products, and phytopathogen resistance. Thus, there is a need to generate alternatives that are safe, ecological, and economically viable to face this problem. Phytopathogen inhibition in fruit utilizing antagonist microorganisms has been recognized as a type of biological control (BC), which could represent a viable and environmentally safe alternative to synthetic fungicides. Despite the ecological benefit that derives from the use of controllers and biological control agents (BCA) at a commercial level, their application and efficient use has been minimal at a global level.

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“…The NOAEL for Ni depends on the compound tested, and ranges from 10 µM for NiCl 2 on human keratinocytes [ 14 ] to 100 µM for NiCl 2 -6H 2 O on BALB/3T3 cells [ 15 ] and 150 μM for Ni(NO 3 ) 2 on rat liver cells [ 16 ]. The oral reference dose of nickel would suggest a benchmark dose of 4–5 mg Ni/kg/day, based on increased prenatal mortality due to the oral ingestion of nickel sulfate and nickel chloride [ 17 ].…”

Section: Discussionmentioning

confidence: 99%

Ion Release and Surface Changes of Nickel–Titanium Archwires Induced by Changes in the pH Value of the Saliva—Significance for Human Health Risk Assessment

et al. 2022

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The aim of this study was to explore whether changes in the salivary pH influence mechanical properties, surface roughness, and ion release from NiTi archwires with various surface coatings, and discuss the clinical significance of the findings. The uncoated, rhodium-coated, and nitrified NiTi wires were immersed into artificial saliva of different pH values (4.8, 5.1, 5.5, and 6.6). Released nickel and titanium ions were measured with inductively coupled plasma-optical emission spectroscopy at the end of 28 days. Atomic force microscopy was used to measure the arithmetic average surface roughness Ra, the root-mean-square roughness Rq, and the maximum height of the asperities RZ. The nanoindentation hardness (HIT) and Young’s modulus (EIT) measurements were performed. The change in the pH of artificial saliva is inversely proportional to the release of titanium from both coated and uncoated wires, and the release of nickel from uncoated wires. The surface roughness parameters of both coated and uncoated wires are unaffected by the change in the pH of artificial saliva. The change in the pH of saliva has minor influence on the hardness and Young’s modulus of elasticity of both coated and uncoated wires. The concentration of released metal ions measured was below the recommended upper limit for daily intake; nevertheless, hypersensitivity effects cannot be excluded, even at lower concentrations and at low pH.

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Cell cycle and transmembrane mitochondrial potential analysis after treatment with chromium(iii), iron(iii), molybdenum(iii) or nickel(ii) and their mixtures

Abstract: The aim of this study was to examine the effect of chromium(iii), iron(iii), molybdenum(iii) and nickel(ii) and their combinations on the cell cycle and mitochondrial transmembrane potential (MTP) in BALB/3T3 and HepG2 cells.

Cited by 17 publications

References 27 publications

Toxicity and Metabolomic Impact of Cobalt, Chromium, and Nickel Exposure on HepaRG Hepatocytes

Toxicity and Metabolomic Impact of Cobalt, Chromium, and Nickel Exposure on HepaRG Hepatocytes

Biocontrol of Postharvest Fruit Fungal Diseases by Bacterial Antagonists: A Review

Ion Release and Surface Changes of Nickel–Titanium Archwires Induced by Changes in the pH Value of the Saliva—Significance for Human Health Risk Assessment

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