Cell cycle and the differential expression of HLA-A,B and HLA-DR antigens on human B lymphoid cells.

Abstract

Monoclonal antibodies specific to HLA antigens and the fluorescence-activated cell sorter were used to analyze the changes in the density of human histocompatibility antigens HLA-AB and HLA-DR on the surface of synchronously growing WI-L2 cells (a human B cell line) progressing through the cell cycle. The WI-L2 cells were synchronized by densitydependent arrest in GI, and samples from Go, G1, late S and late G2 phases were used to determine the frequency distribution of cell volume, DNA content, and the relative amounts of cell surface HLA antigens; the observed density changes were calculated from these values. The HLA-AB density remained nearly constant throughout the cell cycle, whereas the HLA-DR density increased sharply at the G2-M stage. These results suggest a cell cycle-dependent differential control of the expression of these two sets of histocompatibility antigens on B cells.

Association between colony forming units-granulocyte macrophage expression of Ia-like (HLA-DR) antigen and control of granulocyte and macrophage production. A new role for prostaglandin E.

“…Monoclonal antibody analysis of human CFU-GM Ia-antigen expression by other laboratories (5,6) have demonstrated the presence of these antigens on virtually all CFU-GM, and are in contrast with the cyclerelated detection by the antibodies used in this and other studies (8,11). Heterogeneity of Ia-antigen expression (20) and changes in HLA-DR antigen density (21) relative to the cell cycle have been reported for both murine and human B-cell lines. The masking and unmasking of epitopic regions or changes in antigen density can result in monoclonal antibodies with different specificities.…”

“…However, investigation of these effects directly in the agar cultures is severely impaired by the semisolid agar matrix. The addition of prostaglandin E and acidic isoferritins to agar cultures at time points [12][13][14][15][16][17][18][19][20][21][22][23][24] h after initiation has no effect on CFU-GM proliferation (unpublished observation). This result may occur as a result of loss of CFU-GM Ia-antigen expression (8,11).…”

Activation of MHC Class I, II, and CD40 Gene Expression by Histone Deacetylase Inhibitors

“…MHC class II, but not class I, has been reported (29,30) to be regulated differentially during the cell cycle. We therefore analyzed the cell cycle of our tumor lines (by DNA staining and flow cytometry) with each of the treatment conditions under study.…”

Quantitative variation of the common acute lymphoblastic leukemia antigen (gp100) on leukemic marrow blasts.

“…In view of the wide quan'-titative variation of CALLA expression revealed by FCM analysis, we propose that cases with only heavy chain gene rear- In each of the 35 CALLA+ cases, the antigen was detected on G0/G1-phase as well as S phase blasts, demonstrating that its presence does not depend on cell cycle phase. Nonetheless, we recorded appreciable increases of CALLA as blasts moved from GO/GI into S. Unlike the kinetics of several other cell surface antigens, including HLA-DR (18)(19)(20), this change does not appear to be under independent cell cycle control, as the net increase of CALLA in S phase corresponded approximately to increased cell size (surface area). GO/G1 reflects an increased uniformity ofCALLA measurement within this well-defined region ofthe cell cycle.…”

“…We reasoned from studies with other differentiation antigens (16)(17)(18)(19)(20) that quantitation of CALLA on leukemic blasts might disclose substantial differences in its expression, which could be related to the predicted pattern of differentiation within the B precursor pathway (15). Additionally, since about one-third…”