‘Cell cycle’ and ‘cell death’- related genes are differentially expressed during long – term in vitro real-time cultivation of porcine oviductal epithelial cells

Abstract

Alterations in cells depend on their genetic material, its activation and translation of the products. The genes responsible for the cell cycle processes and apoptosis of porcine oviductal cells have been presented in our study. The processes occurring in the reproductive system of females are extremely complex and require in-depth knowledge. Thanks to in vitro studies on the fallopian tube epithelium cells, we can get closer to understanding the biochemical and morphological changes occurring in mammalian organisms. Our research was conducted on fallopian tubes obtained from commercially bred pigs and its aim was to assess the expression profile of genes responsible for the most important processes of cellular life. Cell cultures were carried out for 30 days, with the obtained cells subjected to molecular analysis. We have shown significant regulation of “cell death” and “cell cycle” genes, some of which are related to the reproductive system. The alterations in transcriptomic profile and mutual relations between the genes were analyzed and related to the literature findings. The knowledge gained could help in identifying new potential markers of the in vitro occurrence of processes described by the ontology groups of interest.Running title: pig, oocytes, microarray assays, in vitro maturation (IVM)

New Gene Markers Expressed in Porcine Oviductal Epithelial Cells Cultured Primary In Vitro Are Involved in Ontological Groups Representing Physiological Processes of Porcine Oocytes

“…SERPINB2 , also called plasminogen activator inhibitor type 2 [ 30 ], is one of the most upregulated proteins after cellular stress [ 31 ]. Decreased expression of this gene was also noted during culture of porcine OECs [ 6 ], while up-regulation was demonstrated upon in vitro culture of porcine granulosa cells [ 22 ].…”

“…Recently, it was observed that oviductal tissue, including epithelium, may be cultured in vitro for short and long-term periods [ 4 ]. In oviductal epithelial cell (OEC) in vitro culture, cells may change their morphology and biochemical properties [ 5 , 6 ]. The biochemical and molecular changes involve modifications of gene and protein expression profiles that may regulate gamete-OEC interactions [ 2 ].…”

Human Cumulus Cells in Long-Term In Vitro Culture Reflect Differential Expression Profile of Genes Responsible for Planned Cell Death and Aging—A Study of New Molecular Markers

“…Expression of oocyte and CC genes can be altered by changes in their cell cycle and metabolism or induction of apoptosis [36]. Several pro-and anti-apoptotic genes, such as BCLAF1 and XAF1, have been already identified that play an important role in the follicle's survival, atresia, and apoptosis [37,38].…”

Potential of aquaporins and connexins in dogs and their relation to the reproductive tract

“…Various correlations and communication paths between the granulosa cells (GCs) surrounding the oocyte and the ovum, as well as between GCs and oviduct epithelial cells (OECs) (35), indicate that it is the intercellular interactions that might be responsible for the developmental competence of COCs. This in turn could result in the ability to fertilize and produce offspring.…”