Cell Cycle Actions of Parathyroid Hormone-Related Protein in Non-Small Cell Lung Carcinoma.

Combinatorial library discovery of small molecule inhibitors of lung cancer proliferation and parathyroid hormone-related protein expression

“…The three PTHrP promoters, P1, P2 and P3, were studied with a full length PTHrP promoter-luciferase reporter construct provided by Dr. Zhor Bouizar (INSERM, Paris, France). Cells were transfected with promoter construct and a beta-galactosidase construct (lacZ) to allow normalization of transfection efficiency by a standard cationic liposome method 22 and harvested 48 h post-transfection in reporter lysis buffer (Promega Corporation, Madison, WI). Luciferase activity was determined with a luminometer (MGM Instruments, Hamden, CT) in a mixture of 20 μl of cell extract and beetle luciferin (Promega), compared to background activity in cells transfected with the promoterless luciferase vector pGL-2.…”

Role of PTHrP nuclear localization and carboxyl terminus sequences in postnatal spinal cord development

“…Additionally, spinal cord growth depends on the rates of cell‐proliferating and salvaging processes (Molina & Pituello, 2017). Putative PTHrP downstream signaling associated with cell‐division cycles may include cyclins, cyclin‐dependent kinases (CDK), and cyclin‐dependent kinase inhibitors (CDKI) (Calvo et al., 2014; Hastings et al., 2009). In addition, B lymphoma Mo‐MLV insertion region 1 homolog (Bmi‐1) is another cell cycle inhibitor gene that is related to the activity of PTHrP (Miao et al., 2008; Zhou et al., 2016).…”