Cell cultures from the symbiotic soft coral Sinularia flexibilis

Khalesi, Mohammad

doi:10.1007/s11626-008-9128-7

Cited by 19 publications

(13 citation statements)

References 31 publications

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“…separating cells from each other. The enzymes involved target cell-cell and cell-extracellular matrix bonds [97,101] and each method requires adjusting treatment parameters to cell type. This may involve increasing the tissue surface to provide a large contact surface area for enzyme activity, and enzymatic digestion of extracellular matrix components and cleaving cell-cell contacts.…”

Section: Cell Dissociationmentioning

confidence: 99%

Applying model approaches in non-model systems: A review and case study on coral cell culture

et al. 2021

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Model systems approaches search for commonality in patterns underlying biological diversity and complexity led by common evolutionary paths. The success of the approach does not rest on the species chosen but on the scalability of the model and methods used to develop the model and engage research. Fine-tuning approaches to improve coral cell cultures will provide a robust platform for studying symbiosis breakdown, the calcification mechanism and its disruption, protein interactions, micronutrient transport/exchange, and the toxicity of nanoparticles, among other key biological aspects, with the added advantage of minimizing the ethical conundrum of repeated testing on ecologically threatened organisms. The work presented here aimed to lay the foundation towards development of effective methods to sort and culture reef-building coral cells with the ultimate goal of obtaining immortal cell lines for the study of bleaching, disease and toxicity at the cellular and polyp levels. To achieve this objective, the team conducted a thorough review and tested the available methods (i.e. cell dissociation, isolation, sorting, attachment and proliferation). The most effective and reproducible techniques were combined to consolidate culture methods and generate uncontaminated coral cell cultures for ~7 days (10 days maximum). The tests were conducted on scleractinian corals Pocillopora acuta of the same genotype to harmonize results and reduce variation linked to genetic diversity. The development of cell separation and identification methods in conjunction with further investigations into coral cell-type specific metabolic requirements will allow us to tailor growth media for optimized monocultures as a tool for studying essential reef-building coral traits such as symbiosis, wound healing and calcification at multiple scales.

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Section: Cell Dissociationmentioning

confidence: 99%

Applying model approaches in non-model systems: A review and case study on coral cell culture

et al. 2021

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“…Some successes with both scleractinian and soft coral cell culture have been achieved (Domart‐Coulon et al . ; Khalesi ; Reyes‐Bermudez & Miller ; Mass et al . ), but this method requires a more focused and larger effort to assess its usefulness as a system to study the coral microbiome.…”

Section: A Model System For Coralsmentioning

confidence: 99%

Coral—the world's most diverse symbiotic ecosystem

2015

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Zooxanthellate corals (i.e. those harbouring Symbiodinium) are the main builders of the world's shallow-water marine coral reefs. They represent intimate diverse symbioses between coral animals, single-celled photosynthetic dinoflagellates (Symbiodinium spp.), other microscopic eukaryotes, prokaryotes and viruses. Crabs and other crustaceans, worms, sponges, bivalves and hydrozoans, fishes, sea urchins, octopuses and sea stars are itinerant members of these 'rainforests of the sea'. This review focuses on the biodiversity of scleractinian coral animals and their best studied microscopic epi-and endosymbionts. In relation to coral-associated species diversity, Symbiodinium internal transcribed spacer region sequence types tally 10 2 -10 3 or up tõ 15 different operational taxonomic units (OTUs, or putative species at the 97% sequence identity level; this cut-off was chosen based on intragenomic sequence diversity observed in monoclonal cultures) and prokaryotes (mostly bacterial) total 10 2 -10 4 OTUs. We analysed all publically accessible 16S rRNA gene sequence data and found Gammaproteobacteria were extremely abundant, followed by Alphaproteobacteria. Notably, Archaea were poorly represented and 'unassigned OTUs' were abundant in data generated by high-throughput DNA sequencing studies of corals. We outline and compare model systems that could be used in future studies of the coral holobiont. In our future directions, we recommend a global coral sampling effort including substantial attention being paid to method of coral tissue acquisition, which compartments (mucus, tissue, skeleton) to explore, broadening the holobiont members considered and linking biodiversity with functional investigations.

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“…Clusters of cells have been reported to form in suspension in several models, including embryonic cells (Reyes-Bermudez et al 2009) as well as adult cells (Kopecky and Ostrander 1999;Nesa and Hidaka 2009) or in adherent cultures on Primaria substrate (Domart-Coulon et al 2001;Helman et al 2008;Mass et al 2012). In the soft coral Sinularia flexibilis, these aggregates were interpreted as a sign of cell proliferation (Khalesi et al 2008). Recent encouraging results have reported regeneration of polyps from suspended spheroid tissue cultured in seawater (Vizel et al 2011).…”

Section: Introductionmentioning

confidence: 99%