Cell culture on hydrophilicity-controlled silicon nitride surfaces

Abstract

Cell culture on silicon nitride membranes is required for atmospheric scanning electron microscopy, electron beam excitation assisted optical microscopy, and various biological sensors. Cell adhesion to silicon nitride membranes is typically weak, and cell proliferation is limited. We increased the adhesion force and proliferation of cultured HeLa cells by controlling the surface hydrophilicity of silicon nitride membranes. We covalently coupled carboxyl groups on silicon nitride membranes, and measured the contact angles of water droplets on the surfaces to evaluate the hydrophilicity. We cultured HeLa cells on the coated membranes and evaluated stretch of the cell. Cell migration and confluence were observed on the coated silicon nitride films. We also demonstrated preliminary observation result with direct electron beam excitation-assisted optical microscope.

<p>Magnesium-doped Nanostructured Titanium Surface Modulates Macrophage-mediated Inflammatory Response for Ameliorative Osseointegration</p>

“…It has been reported that high hydrophilicity of the substrates can improve cell spreading, adhesion, and proliferation. 57 The in vitro data showed that MgN manipulated macrophage polarization and enhanced osteoimmunomodulation. However, we have to point out that immune responses are complicated, and cannot be easily mimicked in in vitro experiments.…”

“…It has been reported that high hydrophilicity of the substrates can improve cell spreading, adhesion, and proliferation. 57 …”

Micro‐nano porous structured tantalum‐coated dental implants promote osteogenic activity in vitro and enhance osseointegration in vivo

“…From the perspective of bionics, the morphology of micro‐nano porous structure was similar with the structure of innate extracellular matrices, and some irregular holes size resembled to the epithelial basement membrane 40 . These similarities are effective in emulating cell functions, such as the adhesion on internalized surface structure with cellular antennas and micropores 40,41 . Furthermore, disordered nanostructure seems to be more conducive to matrix mineralization and bone formation than ordered nanostructures 35 .…”

Electrochemical Behavior and Detection of Diclofenac at a Microporous Si3N4 Membrane Modified Water–1,6-dichlorohexane Interface System

“…Additionally, the observed behavior of the voltammograms can be attributed to the location of the liquid-liquid interface in the pore. Silicon nitride, Si 3 N 4 , by virtue of its chemical composition is hydrophobic, the contact angles for water and DCH is about the same on as-received Si 3 N 4 membrane in the range 60-80 • [53][54][55]. Thus, the filling procedure could play an important role for the determination of kinetic parameters from the electrochemical measurements at a liquid-liquid micro-interface.…”