Cell culture on AAO nanoporous substrates with and without geometry constrains

Hu, Jie; Tian, J. H.; Shi, Jian; Zhang, F.; He, Delong; Liu, L.; Jung, Dongju; Bai, Jing; Chen, Y.

doi:10.1016/j.mee.2010.12.055

Cited by 20 publications

(13 citation statements)

References 15 publications

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“…In contrast, cell functionality was fostered on membranes with small pore diameters of around 50 nm [21]. These results agree with the results obtained using 50-150 nm PAA and NIH-3T3 fibroblast cells [22], 20-200 nm pore size alumina and human osteoblast-like MG63 cells [23], and 75-300 nm pore size alumina and NIH 3T3 cells [24].…”

Section: Cell Growthsupporting

confidence: 90%

Mesoporous alumina as a biomaterial for biomedical applications

Xifré-Pérez

Ferre-Borull

Pallarès

et al. 2015

Open Material Sciences

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Porous anodic alumina (PAA) is a biomaterial based on a cost-effective electrochemical anodization of pure aluminum with unique geometrical properties, i.e., self-ordering hexagonal pore distribution, tunable pore diameters and interpore distances, and uniformity of the pores in the vertical direction (nanochannels). These remarkable properties have found important applications in several fields such as energy storage, optics, photonics, magnetism, catalysis and, in particular, in the biomedicine field. In this work, we review the current state of research and key issues on cell culture and implants, drug delivery systems with complex release profiles and specific action, and high efficiency and sensitivity biosensors with different biosensing mechanisms, all of them based on PAA. The biocompatibility, morphology of the surface, nanoestructural engineering in-depth, surface functionalization and coatings are discussed and analyzed in detail.

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Section: Cell Growthsupporting

confidence: 90%

Mesoporous alumina as a biomaterial for biomedical applications

Xifré-Pérez

Ferre-Borull

Pallarès

et al. 2015

Open Material Sciences

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“…[14][15][16][17][18][19] Recently, Wang et al and Hu et al studied NIH 3T3 (fibroblasts) and PC12 (pheochromocytoma) cellular behaviors on PAA with different pore sizes, but the nanopore arrays used in their study were in a disordered arrangement and pore size distribution was not even. [20][21][22] Dalby et al have previously proved that nanoscale disorder on substrates also directly influenced cellular behaviors. 23 Thus, the disorder of the porous arrangement may interfere with cell sensing of the different sized pores on PAA in their study.…”

Section: Introductionmentioning

confidence: 99%

Understanding improved osteoblast behavior on select nanoporous anodic alumina

Yan

et al. 2014

IJN

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The aim of this study was to prepare different sized porous anodic alumina (PAA) and examine preosteoblast (MC3T3-E1) attachment and proliferation on such nanoporous surfaces. In this study, PAA with tunable pore sizes (25 nm, 50 nm, and 75 nm) were fabricated by a two-step anodizing procedure in oxalic acid. The surface morphology and elemental composition of PAA were characterized by field emission scanning electron microscopy and X-ray photoelectron spectroscopy analysis. The nanopore arrays on all of the PAA samples were highly regular. X-ray photoelectron spectroscopy analysis suggested that the chemistry of PAA and flat aluminum surfaces were similar. However, contact angles were significantly greater on all of the PAA compared to flat aluminum substrates, which consequently altered protein adsorption profiles. The attachment and proliferation of preosteoblasts were determined for up to 7 days in culture using field emission scanning electron microscopy and a Cell Counting Kit-8. Results showed that nanoporous surfaces did not enhance initial preosteoblast attachment, whereas preosteoblast proliferation dramatically increased when the PAA pore size was either 50 nm or 75 nm compared to all other samples ( P <0.05). Thus, this study showed that one can alter surface energy of aluminum by modifying surface nano-roughness alone (and not changing chemistry) through an anodization process to improve osteoblast density, and, thus, should be further studied as a bioactive interface for orthopedic applications.

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“…23 A higher proliferation rate was detected in NIH/3T3 cells cultured on alumina with 75 nm pores than in those cultured on alumina with 300 nm pores. 24 Although the cell types differed, higher cell viability was consistently observed in cells cultured on alumina substrate with smaller pore sizes. In other studies in which MSCs were cultured on TiO 2 nanotubes with varying diameters, higher cell viability was observed in nanotubes with a smaller diameter (15 nm).…”

Section: Discussionmentioning

confidence: 98%

In vitro proliferation and osteogenic differentiation of mesenchymal stem cells on nanoporous alumina

Song¹,

Ju²,

Song³

et al. 2013

IJN

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Cell adhesion, migration, and proliferation are significantly affected by the surface topography of the substrates on which the cells are cultured. Alumina is one of the most popular implant materials used in orthopedics, but few data are available concerning the cellular responses of mesenchymal stem cells (MSCs) grown on nanoporous structures. MSCs were cultured on smooth alumina substrates and nanoporous alumina substrates to investigate the interaction between surface topographies of nanoporous alumina and cellular behavior. Nanoporous alumina substrates with pore sizes of 20 nm and 100 nm were used to evaluate the effect of pore size on MSCs as measured by proliferation, morphology, expression of integrin β1, and osteogenic differentiation. An MTT assay was used to measure cell viability of MSCs on different substrates, and determined that cell viability decreased with increasing pore size. Scanning electron microscopy was used to investigate the effect of pore size on cell morphology. Extremely elongated cells and prominent cell membrane protrusions were observed in cells cultured on alumina with the larger pore size. The expression of integrin β1 was enhanced in MSCs cultured on porous alumina, revealing that porous alumina substrates were more favorable for cell growth than smooth alumina substrates. Higher levels of osteoblastic differentiation markers such as alkaline phosphatase, osteocalcin, and mineralization were detected in cells cultured on alumina with 100 nm pores compared with cells cultured on alumina with either 20 nm pores or smooth alumina. This work demonstrates that cellular behavior is affected by variation in pore size, providing new insight into the potential application of this novel biocompatible material for the developing field of tissue engineering.

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Cell culture on AAO nanoporous substrates with and without geometry constrains

Cited by 20 publications

References 15 publications

Mesoporous alumina as a biomaterial for biomedical applications

Mesoporous alumina as a biomaterial for biomedical applications

Understanding improved osteoblast behavior on select nanoporous anodic alumina

In vitro proliferation and osteogenic differentiation of mesenchymal stem cells on nanoporous alumina

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