Cell culture of small round cell tumor originating in the thoracopulmonary region. Evidence for derivation from a primitive pluripotent cell

Fujii, Yasuhisa; Hongo, Teruaki; Nakagawa, Yuichi; Nasuda, Kaoru; Mizuno, Yohei; Igarashi, Y; Naito, Yasuhisa; Maeda, Matsuyoshi

doi:10.1002/1097-0142(19890701)64:1<43::aid-cncr2820640109>3.0.co;2-f

Cited by 44 publications

(12 citation statements)

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“…About 10% of cases with ESFT express EWS-ERG (Kaneko et al 1997). The translocation t(11;22)(q24;q12) was also detected in the reported EES cell lines (Whang-Peng et al 1986;Fujii et al 1989;Homma et al 1989). Karyotype analysis of our established EES1 revealed chromosomes with translocation of t(11;22)(q24:q12).…”

mentioning

confidence: 56%

“…There were no significant associations between cytokeratin expression and each of the following parameters: patient age, sex, skeletal and extraskeletal primary site. Fujii et al (1989) and Llombart-Bosch et al (1990) reported positivity of their established EES cells for cytokeratin. In the present case, the primary tumor did not express keratin but the xenograft was reactive with keratin.…”

mentioning

confidence: 98%

“…The extensive literature survey reveals that only ten cell lines of EES or PNET have been reported so far (Whang-Peng et al 1986;Fujii et al 1989;Homma et al 1989;Llombart-Bosch et al 1990;Hara et al 1991). Whang-Peng et al (1986) evaluated long cultured EES and the other small, round, blue-cell tumors including rhabdomyosarcoma, Ewing's sarcoma of bone, and mesenchymal chondrosarcoma.…”

mentioning

confidence: 99%

“…Whang-Peng et al (1986) evaluated long cultured EES and the other small, round, blue-cell tumors including rhabdomyosarcoma, Ewing's sarcoma of bone, and mesenchymal chondrosarcoma. Fujii et al (1989) described a cell line of EES obtained from the chest wall lesion in a 14-year-old girl. Homma et al (1989) reported a cell line designated SCCH-196 established from an extraskeletal small round cell sarcoma developed in a 16-yearold Japanese girl.…”

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confidence: 99%

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Establishment and Characterization of a Clonal Human Extraskeletal Ewing's Sarcoma Cell Line, EES1

Hatori

Doi

Watanabe

et al. 2006

Tohoku J. Exp. Med.

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Ewing's sarcoma, a small round cell sarcoma arising in soft tissue as well as the bone, is one of the most malignant tumors in children and young adults. Few established cell lines of extraskeletal Ewing's sarcoma (EES) have been reported, which made it difficult to examine the biological features of EES. Therefore, we have established a new clonal cell line of EES. We report its morphological characters, results of chromosomal and immunohistochemical analysis. A piece of tumor obtained from the 18-year-old female patient with EES was xenografted in a nude mouse. In vitro subcultured cells were then obtained from this xenograft. A clonal cell line was subsequently established by limiting dilution and designated EES1. EES1 cells had a doubling time of 24 hours. In the xenografted tumor, the cells expressed vimentin, CD99 (MIC2), neuron specific enolase (NSE) and cytokeratin. The original tumor cells also expressed vimentin, CD 99, and NSE, but was negative for cytokeratin. The morphological and immunohistochemical features of this cell line established, except for cytokeratin expression, were consistent with those of the primary tumor. Cytogenetic analysis of EES1 revealed chromosomal translocation of t(11; 12)(q24;ql2). The chimeric fusion of the Ewing's sarcoma gene in band 22q12 with the Friend leukemia virus integration-1 gene in band 11q24 was also demonstrated. Fluorescence in situ hybridization further confirmed the presence of translocation involving the Ewing's sarcoma gene in both the primary tumor and EES1 cells. In conclusion, we have established a human EES cell line EES1, which will provide a useful model for studying various aspects of human EES.

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confidence: 56%

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confidence: 98%

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Establishment and Characterization of a Clonal Human Extraskeletal Ewing's Sarcoma Cell Line, EES1

Hatori

Doi

Watanabe

et al. 2006

Tohoku J. Exp. Med.

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“…Immunoglobulin (Ig) subclass monoclonal antibodies were determined by immunodiffusion by rabbit anti-mouse Ig subclass antisera (BIO RAD Laboratories). Source of tumours Surgically resected human tumours, tumours maintained in a nude mice heterotransplantation system and human cell lines maintained in culture medium were used in this study (Table I) Medicine, Shizuoka;Fujii et al, 1989). shown to have chromosomal translocation with t(I 1; 22).…”

Section: Methodsmentioning

confidence: 99%

A monoclonal antibody specifically reactive with Ewing's sarcoma

Hara¹,

Ishii²,

Tanaka³

et al. 1989

Br J Cancer

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Ewing's sarcoma (ES) belongs morphologically to the group of small round cell tumours of children because of the absence of unequivocal features of differentiation. This tumour arises from bone and soft tissue, and the histogenesis is still unknown. By electron microscopy, several characteristic features were reported, but specific features of diagnostic value have not been found. Although several monoclonal and polyclonal antibodies were applied to ES, no membrane antigen or protein were known to be specific for ES, and thus the immunodiagnosis of this tumour has been uncertain (Donner et al., 1985;Triche et al., 1986). In this study, we have established a mouse monoclonal antibody, 5Cl which specifically reacts with ES. The unique distribution and partial biochemical characterisation of 5CI1 -defined molecule are reported. Materials and methodsDevelopment of mouse monoclonal antibodies ES cell line RD-ES (obtained from American Type Culture Collection (ATCC)) cells (5 x 107 cells) were injected into the intraperitoneal cavity of Balb/cA mice at 2-week intervals three times. Four days after the last boost, immune splenocytes were fused with NS-1 mouse myeloma cells. Supernatants of growing hybridomas were screened on acetone-fixed frozen sections of CR-EW1 tumour, and ES line heterotransplanted in the nude mice by the indirect immunoperoxidase method described below. Hybridomas secreting antibodies of interest were cloned twice by limiting dilution. Immunoglobulin (Ig) subclass monoclonal antibodies were determined by immunodiffusion by rabbit anti-mouse Ig subclass antisera (BIO RAD Laboratories). Source of tumours Surgically resected human tumours, tumours maintained in a nude mice heterotransplantation system and human cell lines maintained in culture medium were used in this study (Table I) Preparation of normal andfetal tissues Normal tissues were obtained from surgery and autopsy. Normal fetal tissues of 6-12 weeks gestational age which were obtained at the time of necropsy after spontaneous and legal abortion were frozen and stored as described above.

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Soft tissue Ewing's sarcoma: Characterization in established cultures and xenografts with evidence of a neuroectodermic phenotype

Llombart‐Bosch

Cardá

Peydró-Olaya

et al. 1990

Cancer

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This study characterizes the histogenesis of soft tissue Ewing's sarcoma (StEs) based upon an analysis of three tumors. Long-term cultured cell lines and nude mice xenografts were established from original neoplasms or from their metastases. Histologically they revealed a small round cell pattern without signs of differentiation. Several ultrastructural features of neural type were found; the same were also seen on culture cell lines. Moreover, immunohistochemical study for neural markers revealed the presence of HNK-1, NSE, LIRC-LON 36, S-100 protein, glial fibrillary acidic protein, neurofilaments (70 kilodaltons), and chromogranin; some of these markers were present only in the transplants. Cytokeratin was also seen. The translocation t(11;22)(q24;q12) was found in all three neoplasms together with other chromosomal abnormalities. N-myc RNA gave negative results whereas c-myc RNA was expressed. Therefore it may be postulated that StEs displays neuroectodermal features somewhat similar to those seen in peripheral neuroepithelioma as well as in atypical Ewing's sarcoma of bone.

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Cell culture of small round cell tumor originating in the thoracopulmonary region. Evidence for derivation from a primitive pluripotent cell

Cited by 44 publications

References 26 publications

Establishment and Characterization of a Clonal Human Extraskeletal Ewing's Sarcoma Cell Line, EES1

Establishment and Characterization of a Clonal Human Extraskeletal Ewing's Sarcoma Cell Line, EES1

A monoclonal antibody specifically reactive with Ewing's sarcoma

Soft tissue Ewing's sarcoma: Characterization in established cultures and xenografts with evidence of a neuroectodermic phenotype

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