Cell culture of human gingival fibroblasts, oral cancer cells and mesothelioma cells with serum-free media, STK1 and STK2

Abstract

Abstract. The majority of cells are cultured with Dulbecco's modified Eagle's medium (DMEM) or RPMI supplemented with fetal bovine serum (FBS), which contains numerous factors, including cytokines, nutrients and unknown growth factors. These factors may affect cell growth, apoptosis and differentiation. The serum-free medium, STK2, has been previously reported as suitable for the cell culture of human mesenchymal stem cells. However, how STK1 or STK2 affect the cell proliferation of normal and cancer cells remains unknown. The present study examined the growth of the human gingival fibroblast (HGF-1) cell-line and the HSC-3, CA9-22 and MSTO cancer cell-lines, cultured with STK1 and STK2. STK1 increased the cell proliferation of HGF-1 compared to DMEM by assessment with the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay, whereas STK1 and STK2 markedly inhibited the cell proliferation of HSC-3 and MSTO. The cell proliferation rate of CA9-22 cultured with STK1 or STK2 for 96 h was ~2-fold higher than the rate for 24 h culture. The shape of the HSC-3 cells was also found to have changed to round when cultured with STK2. These results indicate that STK1 increased the cell proliferation of HGF-1 compared to DMEM, whereas the proliferation of HSC-3 and MSTO was inhibited by STK1 and STK2. Thus, STK1 and STK2 had different affects on the cell growth of HGF-1, CA9-22, HSC-3 and MSTO.

Cooperation of Sox4 with β-catenin/p300 complex in transcriptional regulation of the Slug gene during divergent sarcomatous differentiation in uterine carcinosarcoma

“…To induce EMT in Em Ca cells, the three cell lines, including Ishikawa, Hec251, and Hec6 cells, were cultured in STK2, a serum-free medium for mesenchymal stem cells [ 22 ]. As shown in Fig.…”

“…STK2, which is a serum-free culture medium for mesenchymal stem cells, [ 22 ] was obtained from DS Pharma Biomedical (Osaka, Japan).…”

Inhibition of growth and migration of oral and cervical cancer cells by citrus polyphenol

“…17,18 The cell lines used in this study were a human gingival squamous carcinoma cell line, Ca9-22, and a human cervical epithelioid carcinoma cell line, HeLa. The cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) with 10% fetal bovine serum (FBS; Biological Industries, Kibbutz Beit-Haemek, Israel), with the culture dish incubated at 37 C in an atmosphere of 95% air and 5% CO 2 .…”

Hypoxia-mediated cancer stem cells in pseudopalisades with activation of hypoxia-inducible factor-1α/Akt axis in glioblastoma

“…Cells (x10 3 ) were plated in low cell binding plates (Thermo Fisher Scientific, Yokohama, Japan) in STK2 medium, a serum-free culture medium for mesenchymal stem cells, [27] or Eagle's MEM with 10% bovine calf serum.…”