Cell culture distribution in a three-dimensional porous scaffold in perfusion bioreactor

Magrofuoco, Enrico; Flaibani, Marina; Giomo, Monica; Elvassore, Nicola

doi:10.1016/j.bej.2019.02.023

Cited by 13 publications

(12 citation statements)

References 41 publications

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“…Compared to static seeding, dynamic seeding methods, which apply an external force to facilitate cell penetration, such as flow perfusion, centrifugation, orbital shaking, and spinner flask, have shown to result in better cell distribution on scaffolds fabricated with conventional techniques [15][16][17][18][19] . Moreover, culture of these 3D scaffolds in perfusion bioreactors after static seeding has also demonstrated to enhance cell distribution and tissue formation [20] . However the use of bioreactors require specific lab equipment and expertise, and perfusion flow right after seeding results in significant decrease in cell number in AM 3D scaffolds [21] .…”

Section: Introductionmentioning

confidence: 99%

Improving cell distribution on 3D additive manufactured scaffolds through engineered seeding media density and viscosity

et al. 2020

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Section: Introductionmentioning

confidence: 99%

Improving cell distribution on 3D additive manufactured scaffolds through engineered seeding media density and viscosity

et al. 2020

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“…With perfusion and sparse 3D structures, cells survived for 6 weeks [ 62 ] or even for as long as 98 days [ 78 ]. Medium perfusion in the structure [ 79 ] or porous substrates [ 80 , 81 ] allowed a uniform distribution of nutrients and oxygen, with a consequent high viability of cells in the whole structure being observed for a lengthy cultivation period. Cell survival was optimal regardless of its location, whether on channel walls or within a hydrogel [ 82 ].…”

Section: Cellular In Vitro Cardiac Modelsmentioning

confidence: 99%

3D models of dilated cardiomyopathy: Shaping the chemical, physical and topographical properties of biomaterials to mimic the cardiac extracellular matrix

Camman

Joanne

Agbulut

et al. 2022

Bioactive Materials

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The pathophysiology of dilated cardiomyopathy (DCM), one major cause of heart failure, is characterized by the dilation of the heart but remains poorly understood because of the lack of adequate in vitro models. Current 2D models do not allow for the 3D organotypic organization of cardiomyocytes and do not reproduce the ECM perturbations. In this review, the different strategies to mimic the chemical, physical and topographical properties of the cardiac tissue affected by DCM are presented. The advantages and drawbacks of techniques generating anisotropy required for the cardiomyocytes alignment are discussed. In addition, the different methods creating macroporosity and favoring organotypic organization are compared. Besides, the advances in the induced pluripotent stem cells technology to generate cardiac cells from healthy or DCM patients will be described. Thanks to the biomaterial design, some features of the DCM extracellular matrix such as stiffness, porosity, topography or chemical changes can impact the cardiomyocytes function in vitro and increase their maturation. By mimicking the affected heart, both at the cellular and at the tissue level, 3D models will enable a better understanding of the pathology and favor the discovery of novel therapies.

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“…These also usually employ either one or two reservoirs to provide a supply of medium, which allows either recirculation of the same medium or a constant supply of fresh medium. A wide range of different systems have been designed, incorporating different cell supports such as porous membranes [45] , synthetic or extracellular matrix (ECM) scaffolds [46] , [47] , [48] and hydrogels [ 49 , 50 ]. An advantage of these systems is the ease of incorporating them into standard static 3D culture methods, allowing complex tissue equivalents to readily be perfused with medium without needing to simplify other aspects of the culture, as has been demonstrated with full-thickness skin equivalents [ 51 , 52 ].…”

Section: Bioreactor Design and Usagementioning

confidence: 99%

Design considerations of benchtop fluid flow bioreactors for bio-engineered tissue equivalents in vitro

Hoyle

Stenger

2022

Biomaterials and Biosystems

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Cell culture distribution in a three-dimensional porous scaffold in perfusion bioreactor

Cited by 13 publications

References 41 publications

Improving cell distribution on 3D additive manufactured scaffolds through engineered seeding media density and viscosity

Improving cell distribution on 3D additive manufactured scaffolds through engineered seeding media density and viscosity

3D models of dilated cardiomyopathy: Shaping the chemical, physical and topographical properties of biomaterials to mimic the cardiac extracellular matrix

Design considerations of benchtop fluid flow bioreactors for bio-engineered tissue equivalents in vitro

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