Cell culture dimensionality influences mesenchymal stem cell fate through cadherin-2 and cadherin-11

Modular mixing of benzene-1,3,5-tricarboxamide supramolecular hydrogelators allows tunable biomimetic hydrogels for control of cell aggregation in 3D

“…Studying the selfassembly of primary hMSCs for aggregation and spheroid formation has been linked to cadherin expression and the osteogenic, adipogenic, and chondrogenic phenotypes. 63,64 We were interested to investigate if hMSCs aggregate within these hydrogels and the effect of BTA formulations on the cell aggregation and compactness. hMSCs started to aggregate after 6 hours; however, aggregation was slower in the BTA hydrogels compared to the free self-assembly of hMSCs in the agarose mould control.…”

Micro‐Topographies Induce Epigenetic Reprogramming and Quiescence in Human Mesenchymal Stem Cells

“…In recent years, multiple studies found that numerous biomaterial‐induced mechanisms elicit mechanobiological responses in cells. Examples include altered cadherin signaling through cell–cell contacts [ 22 ] or changes in integrin‐mediated signaling through cell–substrate interactions. [ 23 , 24 , 25 , 26 ] An important mechanobiological factor in this regard are changes in cell morphology and cytoskeletal organization, as shown by experiments where inhibiting cytoskeletal‐related signaling abolishes the mechanobiological effects of biomaterials.…”

Advancing regenerative medicine by generating knowledge about the nature of cadherins in human mesenchymal stem cells

“…It reduced mineralized matrix deposition in monolayer culture, in agreement with studies in which cadherin-11-null mutant mice have reduced bone density. 47 The effect of the knockdowns on mineralized matrix deposition was more apparent in monolayer culture compared to aggregate cultures, again revealing the important differences cells experience in these two environments. For adipogenesis, both cadherin-2 and cadherin-11 expression were critical (Figure 6).…”

“…Another cell behavior the cadherins influence is differentiation. 47 We know that under the physiological state, cells tend to express specific cadherin proteins to maintain tissue integrity, while under pathological conditions, cells express inappropriate cadherins, which has a significant effect on cell behavior. For example, a requisite for apical abscission, a critical step during neurogenesis, is the loss of cadherin-2 and when cadherin-2 is persistently expressed it inhibits the apical cellprocess withdrawal.…”

“…48 Cadherin-11, is implicated in adipogenic differentiation because the loss of cadherin-11 inhibits the mesenchymal stem cells from differentiating towards the adipogenic lineage. 47 The involvement of cadherins in crucial differentiation steps makes them vital for timely use to exploit the differentiation potential of stem cells for regenerative medicine.…”