Cell Culture: Designing Microgels for Cell Culture and Controlled Assembly of Tissue Microenvironments (Adv. Funct. Mater. 37/2020)

Caldwell, Alexander S.; Aguado, Brian A.; Anseth, Kristi S.

doi:10.1002/adfm.202070251

Cited by 17 publications

(28 citation statements)

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“…Micro and nanoparticles composed of stimuli responsive polymers paved the way toward several promising applications, such as triggered drug delivery systems, [1][2][3][4][5] materials with advanced optical properties, 6,7 or bioactive coatings that are capable of responding to environmental parameters. [8][9][10][11][12][13] A very prominent type of responsive microparticles are thermosensitive microgels composed of polymers with a lower critical solution temperature (LCST).…”

Section: Introductionmentioning

confidence: 99%

Elastic modulus distribution in poly(N-isopopylacrylamide) and oligo(ethylene glycol methacrylate)-based microgels studied by AFM

et al. 2021

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Section: Introductionmentioning

confidence: 99%

Elastic modulus distribution in poly(N-isopopylacrylamide) and oligo(ethylene glycol methacrylate)-based microgels studied by AFM

et al. 2021

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“…The generation and delivery of mixed particle co‐assemblies has been explored to improve the biodistribution and tissue retention of drug carriers and to deliver multiple cargo types with independent release rates. [ 11,45,46 ] However, the generation of injectable mixed colloidal assemblies with controlled particle suspension dynamics remains a challenge. Based on our results developing MIN6‐HARP cellular suspensions, we hypothesized that HARPs could serve as colloidal scaffolds to improve the suspension dynamics of mixed particle systems through HARP network formation and electrostatic interactions between positively charged chit‐HARPs and negatively charged microspheres.…”

Section: Resultsmentioning

confidence: 99%

“…In contrast, microporous scaffolds that are open to the surrounding tissue environments allow for endogenous cell recruitment throughout the entirety of the scaffold, which is important for tissue remodeling or immunomodulatory applications. [ 11–13 ] Additionally, porous scaffold systems offer advantages for creating 3D cellular suspensions, where cells can receive nutrients from the surrounding environment, interact with one another, and form connections to improve viability and functional outputs. [ 4,12–16 ]…”

Section: Introductionmentioning

confidence: 99%

“…[ 1 ] Hydrogel microparticles or microgels, have been the material of choice for these self‐assembling scaffolds and have shown promise for both 3D cell culture, as well as injectable materials for local tissue remodeling. [ 1,11,20 ] However, microgel scaffold assembly primarily relies on gravitational packing parameters of individual microgel components, which are almost exclusively spherical particles. [ 20 ] This limits the surface area to volume ratio of the microgel scaffolds, which is an important parameter to maximize particle‐cell interactions.…”

Section: Introductionmentioning

confidence: 99%

“…[ 21,22 ] Additionally, most colloidal scaffolds still rely on crosslinking chemistries between the particles, or on being embedded within macroscale hydrogel networks in order to maintain their 3D architectures. [ 1,11,12,23 ] This limitation is again likely due to the spherical morphology of most colloidal hydrogel systems. Electrospun nanofiber meshes provide high surface area to volume ratios and have been used extensively for tissue engineering; however, these nanofiber networks must be implanted and are therefore limited in their broad applicability.…”

Section: Introductionmentioning

confidence: 99%

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Networks of High Aspect Ratio Particles to Direct Colloidal Assembly Dynamics and Cellular Interactions

Finbloom

Demaree

Abate

et al. 2020

Adv Funct Materials

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Injectable colloids that self-assemble into 3D networks are promising materials for applications in regenerative engineering, as they create open systems for cellular infiltration, interaction, and activation. However, most injectable colloids have spherical morphologies, which lack the high material-biology contact areas afforded by higher aspect ratio materials. To address this need, injectable high aspect ratio particles (HARPs) are developed that form 3D networks to enhance scaffold assembly dynamics and cellular interactions. HARPs are functionalized for tunable surface charge through layer-by-layer electrostatic assembly. Positively charged chitosan-HARPs have improved particle suspension dynamics when compared to spherical particles or negatively charged HARPs. Chit-HARPs are used to improve the suspension dynamics and viability of MIN6 cells in 3D networks. When combined with negatively charged gelatin microsphere (GelMS) porogens, chit-HARPs reduce GelMS sedimentation and increase overall network suspension, due to a combination of HARP network formation and electrostatic interactions. Lastly, HARPs are functionalized with fibroblast growth factor 2 (FGF2) to highlight their use for growth factor delivery. FGF2-HARPs increase fibroblast proliferation through a combination of 3D scaffold assembly and growth factor delivery. Taken together, these studies demonstrate the development and diverse uses of high aspect ratio particles as tunable injectable scaffolds for applications in regenerative engineering.

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Pericellular Matrix Formation and Atomic Force Microscopy of Single Primary Human Chondrocytes Cultured in Alginate Microgels

Fredrikson,

Brahmachary,

June

et al. 2023

Advanced Biology

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One of the main components of articular cartilage is the chondrocyte's pericellular matrix (PCM), which is critical for regulating mechanotransduction, biochemical cues, and healthy cartilage development. Here, individual primary human chondrocytes (PHC) are encapsulated and cultured in 50 µm diameter alginate microgels using drop‐based microfluidics. This unique culturing method enables PCM formation and manipulation of individual cells. Over ten days, matrix formation is observed using autofluorescence imaging, and the elastic moduli of isolated cells are measured using AFM. Matrix production and elastic modulus increase are observed for the chondrons cultured in microgels. Furthermore, the elastic modulus of cells grown in microgels increases ≈ten‐fold over ten days, nearly reaching the elastic modulus of in vivo PCM. The AFM data is further analyzed using a Gaussian mixture model and shows that the population of PHCs grown in microgels exhibit two distinct populations with elastic moduli averaging 9.0 and 38.0 kPa. Overall, this work shows that microgels provide an excellent culture platform for the growth and isolation of PHCs, enabling PCM formation that is mechanically similar to native PCM. The microgel culture platform presented here has the potential to revolutionize cartilage regeneration procedures through the inclusion of in vitro developed PCM.

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Cell Culture: Designing Microgels for Cell Culture and Controlled Assembly of Tissue Microenvironments (Adv. Funct. Mater. 37/2020)

Cited by 17 publications

References 0 publications

Elastic modulus distribution in poly(N-isopopylacrylamide) and oligo(ethylene glycol methacrylate)-based microgels studied by AFM

Elastic modulus distribution in poly(N-isopopylacrylamide) and oligo(ethylene glycol methacrylate)-based microgels studied by AFM

Networks of High Aspect Ratio Particles to Direct Colloidal Assembly Dynamics and Cellular Interactions

Pericellular Matrix Formation and Atomic Force Microscopy of Single Primary Human Chondrocytes Cultured in Alginate Microgels

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