Cell Crawling Driven by Spontaneous Actin Polymerization Waves

Kruse, Karsten

doi:10.1007/978-3-319-24448-8_2

Cited by 4 publications

(3 citation statements)

References 75 publications

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“…It has previously been shown that actin polymerization drives the wave-like movement of its upstream nucleator through an autoinhibitory mechanism, 61 which can further generate the directional motion of cells. 62,63 By using asymmetric sawtooth surfaces, we expand this concept by showing that an asymmetric topography can induce esotactic (actin) and microthigmotactic (migrational) behaviors in a cell-type-dependent manner. This dependence of the direction of these phenomena on cell type suggests that multiple regulatory nodes contribute to the fine control of actin dynamics.…”

Section: Resultsmentioning

confidence: 99%

Actin Cytoskeleton and Focal Adhesions Regulate the Biased Migration of Breast Cancer Cells on Nanoscale Asymmetric Sawteeth

et al. 2019

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Physical guidance from the underlying matrix is a key regulator of cancer invasion and metastasis. We explore the effects of surface topography on the migration phenotype of multiple breast cancer cell lines using aligned nanoscale ridges and asymmetric sawtooth structures. Both benign and metastatic breast cancer cells preferentially move parallel to nanoridges, with enhanced speeds compared to flat surfaces. In contrast, asymmetric sawtooth structures unidirectionally bias the movement of breast cancer cells in a cell-type dependent manner. Quantitative analysis shows that the level of bias in cell migration increases when cells move with higher speeds or with higher directional persistence. Live-cell imaging studies further reveal that actin polymerization waves are unidirectionally guided by the sawteeth in the same direction as the cell motion. Highresolution fluorescence imaging and scanning electron microscopy studies reveal that two breast cancer cell lines with opposite migrational profiles exhibit profoundly different cell cortical plasticity and focal adhesion patterns. These results suggest that the overall migration response of cancer cells to surface topography is directly related to the underlying cytoskeletal architectures and dynamics, which are regulated by both intrinsic and extrinsic factors.

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Section: Resultsmentioning

confidence: 99%

Actin Cytoskeleton and Focal Adhesions Regulate the Biased Migration of Breast Cancer Cells on Nanoscale Asymmetric Sawteeth

et al. 2019

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“…Furthermore, we do not claim that the actin waves along the basal membranes of cells are driven solely by the mechanism that we describe. Clearly complex reaction-diffusion feedbacks play an important role in the propagation of actin waves in cells [4,9,25,26,[54][55][56]. Our work may motivate further studies of models that include both the reaction-diffusion dynamics and the membrane shape, coupled by curved membrane complexes that nucleate actin polymerization [15].…”

Section: Discussionmentioning

confidence: 77%

Cell-Substrate Patterns Driven by Curvature-Sensitive Actin Polymerization: Waves and Podosomes

Naoz

Gov

2020

Cells

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Cells adhered to an external solid substrate are observed to exhibit rich dynamics of actin structures on the basal membrane, which are distinct from those observed on the dorsal (free) membrane. Here we explore the dynamics of curved membrane proteins, or protein complexes, that recruit actin polymerization when the membrane is confined by the solid substrate. Such curved proteins can induce the spontaneous formation of membrane protrusions on the dorsal side of cells. However, on the basal side of the cells, such protrusions can only extend as far as the solid substrate and this constraint can convert such protrusions into propagating wave-like structures. We also demonstrate that adhesion molecules can stabilize localized protrusions that resemble some features of podosomes. This coupling of curvature and actin forces may underlie the differences in the observed actin-membrane dynamics between the basal and dorsal sides of adhered cells.

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“…Furthermore, we do not claim that the actin waves along the basal membranes of cells are driven solely by the mechanism that we describe. Clearly complex reaction-diffusion feedbacks play an important role in the propagation of actin waves in cells [3,8,24,25,48,49,50]. Our work may motivate further studies of models that include both the reaction-diffusion dynamics and the membrane shape, coupled by curved membrane complexes that nucleate actin polymerization [14].…”

Section: Discussionmentioning

confidence: 92%

Cell substrate patterns driven by curvature-sensitive actin polymerization: waves and podosomes

Naoz

Gov

2020

Preprint

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Cells adhered to an external solid substrate are observed to exhibit rich dynamics of 1 actin structures on the basal membrane, which are distinct from those observed on the dorsal (free) 2 membrane. Here we explore the dynamics of curved membrane proteins, or protein complexes, that 3 recruit actin polymerization when the membrane is confined by the solid substrate. Such curved 4 proteins can induce the spontaneous formation of membrane protrusions on the dorsal side of cells. 5 However, on the basal side of the cells, such protrusions can only extend as far as the solid substrate 6 and this constraint can convert such protrusions into propagating wave-like structures. We also 7 demonstrate that adhesion molecules can stabilize localized protrusions, that resemble some features 8 of podosomes. This coupling of curvature and actin forces may underlie the differences in the 9 observed actin-membrane dynamics between the basal and dorsal sides of adhered cells.10

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Cell Crawling Driven by Spontaneous Actin Polymerization Waves

Cited by 4 publications

References 75 publications

Actin Cytoskeleton and Focal Adhesions Regulate the Biased Migration of Breast Cancer Cells on Nanoscale Asymmetric Sawteeth

Actin Cytoskeleton and Focal Adhesions Regulate the Biased Migration of Breast Cancer Cells on Nanoscale Asymmetric Sawteeth

Cell-Substrate Patterns Driven by Curvature-Sensitive Actin Polymerization: Waves and Podosomes

Cell substrate patterns driven by curvature-sensitive actin polymerization: waves and podosomes

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