Cell contact but not junctional communication (dye coupling) with biliary epithelial cells is required for hepatocytes to maintain differentiated functions

Mesnil, Marc; Fraslin, Jean-Marc; Piccoli, C.; Yamasaki, Hiroshi; Guguen‐Guillouzo, Christiane

doi:10.1016/0014-4827(87)90292-8

Cited by 96 publications

(34 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…14 Cell-cell arrangements between parenchymal hepatocytes and nonparenchymal cells such as fibroblasts have been suggested to affect the maintenance of differentiated hepatocyte functions by random coculture 15 and patterning co-culture using microfabricated surfaces. 2,16 In our layered system, an endothelial cell sheet co-culture with a hepatocyte layer was produced by using a thermo-responsive polymer grafted surface.…”

Section: Discussionmentioning

confidence: 99%

Novel approach for achieving double‐layered cell sheets co‐culture: overlaying endothelial cell sheets onto monolayer hepatocytes utilizing temperature‐responsive culture dishes

Harimoto

Yamato

Hirose

et al. 2002

J. Biomed. Mater. Res.

224

153

View full text Add to dashboard Cite

Confluent human aortic endothelial cells (HAECs) cultured on thermo-responsive culture dish grafted with poly (N-isopropylacrylamide) were recovered as a contiguous cell sheet. The double-layered co-culture was achieved by placing the recovered HAEC sheet onto the rat hepatocyte layer directly. The double-layered structure of HAEC and hepatocytes remained in tight contact during culture. Hepatocytes in the layered co-culture system with the HAEC sheet maintained the differentiated cell shape and the albumin expression for over 41 days of culture, whereas the functions disappeared within 10 days of culture in control hepatocytes without the HAEC sheet. The layered co-culture of hepatocytes and the HAEC sheets, which allows for the expression of differentiated functions of hepatocyte continuously, such as liver lobule, offers a major advancement in liver tissue engineering.

show abstract

Section: Discussionmentioning

confidence: 99%

Novel approach for achieving double‐layered cell sheets co‐culture: overlaying endothelial cell sheets onto monolayer hepatocytes utilizing temperature‐responsive culture dishes

Harimoto

Yamato

Hirose

et al. 2002

J. Biomed. Mater. Res.

224

153

View full text Add to dashboard Cite

show abstract

“…In the longer term, non-parenchymal cells may continue to proliferate and become a significanrt fraction of the population, with potential capabilities of expressing extracellular matrix components as well as soluble growth factors and cytokines. This may appreciably prolong hepatocyte survival and function, a well known feature of co-cultures of hepatocytes and certain other cell types (Mesnil et al, 1987;Loreal et al, 1993). Both short-and long-term cultures require further assessment in this regard.…”

Section: Hepatocyte Cultures 13mentioning

confidence: 96%

Liver regeneration following partial hepatectomy: genes and metabolism

Bucher

Farmer

1998

Liver Growth and Repair

View full text Add to dashboard Cite

“…A considerable step forward in long-term maintenance of differentiated hepatocytes was made by adding another liver epithelial cell type (126). When cocultured with rat liver epithelial cells probably derived from primitive biliary cells, hepatocytes from various species including humans survive for several weeks and retain various liverspecific functions; these include production of plasma proteins, expression of both phase I and phase II drug metabolizing enzymes, and taurocholate uptake (52,71,(127)(128)(129)(130)(131)(132)(133)(134)(135) (71,126,128) and with preservation of communication through gap junctions (136). These two features could be related because in pure hepatocyte cultures the addition of proteoglycans improves gap junction activity (137).…”

Section: However Survival and Metabolic Compe-mentioning

confidence: 99%

Liver cell models in in vitro toxicology.

Guillouzo

1998

Environ Health Perspect

293

125

View full text Add to dashboard Cite

In vitro liver preparations are increasingly used for the study of hepatotoxicity of chemicals. In recent years their actual advantages and limitations have been better defined. The cell models, slices, and mainly primary hepatocyte cultures, appear to be the most powerful in vitro systems, as liver-specific functions and responsiveness to inducers are retained either for a few days or several weeks depending on culture conditions. Maintenance of phase and phase 11 xenobiotic metabolizing enzyme activities allows various chemical investigations to be performed, including determination of kinetic parameters, metabolic profile, interspecies comparison, inhibition and induction effects, and drug-drug interactions. In vitro liver cell models also have various applications in toxicology: screening of cytotoxic and genotoxic compounds, evaluation of chemoprotective agents, and determination of characteristic liver lesions and associated biochemical mechanisms induced by toxic compounds. Extrapolation of the results to the in vivo situation remains a matter of debate. Presently, the most convincing applications of liver cell models are the studies on different aspects of metabolism and mechanisms of toxicity. For the future, there is a need for better culture conditions and differentiated hepatocyte cell lines to overcome the limited availability of human liver tissues. In addition, strategies for in vitro analysis of potentially toxic chemicals must be better defined. Environ Health Perspect 106(Suppl 2):511-532 (1998). http.//ehpnetl.niehs.nih.gov/docs/1998/Suppl-2/51 1-532guillouzo/abstract.html

show abstract

Cell contact but not junctional communication (dye coupling) with biliary epithelial cells is required for hepatocytes to maintain differentiated functions

Cited by 96 publications

References 21 publications

Novel approach for achieving double‐layered cell sheets co‐culture: overlaying endothelial cell sheets onto monolayer hepatocytes utilizing temperature‐responsive culture dishes

Novel approach for achieving double‐layered cell sheets co‐culture: overlaying endothelial cell sheets onto monolayer hepatocytes utilizing temperature‐responsive culture dishes

Liver regeneration following partial hepatectomy: genes and metabolism

Liver cell models in in vitro toxicology.

Contact Info

Product

Resources

About