Cell compaction influences the regenerative potential of passaged bovine articular chondrocytes in an ex vivo cartilage defect model

Schmutzer, Michael; Aszódi, Attila

doi:10.1016/j.jbiosc.2016.11.002

Cited by 8 publications

(6 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…18,24 However, the creation of articular defects in mice is technically challenging due to the small size of the joint and the narrow thickness of the articular cartilage of mice. 18 The practicality of using ex vivo tissues to examine cartilage repair has been demonstrated in equine, 25 bovine, 26 and human tissue explants. 27,28 Therefore, an ex vivo mouse model might be a useful tool to study the repair and regeneration of osteochondral defects and to form a bridge between cell culture models and in vivo models.…”

Section: Introductionmentioning

confidence: 99%

Development of an Ex Vivo Murine Osteochondral Repair Model

Schaik¹,

Gaul

Dorthé

et al. 2018

CARTILAGE

View full text Add to dashboard Cite

Objective Mouse models are commonly used in research applications due to the relatively low cost, highly characterized strains, as well as the availability of many genetically modified phenotypes. In this study, we characterized an ex vivo murine osteochondral repair model using human infrapatellar fat pad (IPFP) progenitor cells. Design Femurs from euthanized mice were removed and clamped in a custom multidirectional vise to create cylindrical osteochondral defects 0.5 mm in diameter and 0.5 mm deep in both condyles. The IPFP contains progenitors that are a promising cell source for the repair of osteochondral defects. For proof of concept, human IPFP-derived progenitor cells, from osteoarthritic (OA) patients, cultured as pellets, were implanted into the defects and cultured in serum-free medium with TGFβ3 for 3 weeks and then processed for histology and immunostaining. Results The custom multidirectional vise enabled reproducible creation of osteochondral defects in murine femoral condyles. Implantation of IPFP-derived progenitor cells led to development of cartilaginous tissue with Safranin O staining and deposition of collagen type II in the extracellular matrix. Conclusions We showed feasibility in creating ex vivo osteochondral defects and demonstrated the regenerative potential of OA human IPFP-derived progenitors in mouse femurs. The murine model can be used to study the effects of aging and OA on tissue regeneration and to explore molecular mechanisms of cartilage repair using genetically modified mice.

show abstract

Section: Introductionmentioning

confidence: 99%

Development of an Ex Vivo Murine Osteochondral Repair Model

Schaik¹,

Gaul

Dorthé

et al. 2018

CARTILAGE

View full text Add to dashboard Cite

show abstract

“…Articular cartilage is a highly specialized and avascular tissue that is the most common type of cartilage covering the surface of articular joints (Schmutzer & Aszodi, 2017 ). It consists primarily of water (65–80% of wet weight), collagen fibers (10–20% of wet weight and 60% of dry weight, where type II collagens represent 90–95% of the collagen fibers), and proteoglycans (10–15% of wet weight).…”

Section: Cartilagementioning

confidence: 99%

Insights into the present and future of cartilage regeneration and joint repair

Evenbratt¹,

Andreasson

Bicknell³

et al. 2022

Cell Regen

View full text Add to dashboard Cite

Knee osteoarthritis is the most common joint disease. It causes pain and suffering for affected patients and is the source of major economic costs for healthcare systems. Despite ongoing research, there is a lack of knowledge regarding disease mechanisms, biomarkers, and possible cures. Current treatments do not fulfill patients’ long-term needs, and it often requires invasive surgical procedures with subsequent long periods of rehabilitation. Researchers and companies worldwide are working to find a suitable cell source to engineer or regenerate a functional and healthy articular cartilage tissue to implant in the damaged area. Potential cell sources to accomplish this goal include embryonic stem cells, mesenchymal stem cells, or induced pluripotent stem cells. The differentiation of stem cells into different tissue types is complex, and a suitable concentration range of specific growth factors is vital. The cellular microenvironment during early embryonic development provides crucial information regarding concentrations of signaling molecules and morphogen gradients as these are essential inducers for tissue development. Thus, morphogen gradients implemented in developmental protocols aimed to engineer functional cartilage tissue can potentially generate cells comparable to those within native cartilage. In this review, we have summarized the problems with current treatments, potential cell sources for cell therapy, reviewed the progress of new treatments within the regenerative cartilage field, and highlighted the importance of cell quality, characterization assays, and chemically defined protocols.

show abstract

“…However, the sample can be more difficult to postprocess and analyze given multiple materials/tissues of varying compositions. Despite the potential of the ex vivo model, to date it has been rarely used to study a 3D printed full OC scaffold and is more commonly used in chondral-only defects [233][234][235][236][237][238].…”

Section: Functional Evaluation: In Vitro and In Vivomentioning

confidence: 99%

3D Printed Multiphasic Scaffolds for Osteochondral Repair: Challenges and Opportunities

Doyle

Snow

Duchi

et al. 2021

IJMS

View full text Add to dashboard Cite

Osteochondral (OC) defects are debilitating joint injuries characterized by the loss of full thickness articular cartilage along with the underlying calcified cartilage through to the subchondral bone. While current surgical treatments can provide some relief from pain, none can fully repair all the components of the OC unit and restore its native function. Engineering OC tissue is challenging due to the presence of the three distinct tissue regions. Recent advances in additive manufacturing provide unprecedented control over the internal microstructure of bioscaffolds, the patterning of growth factors and the encapsulation of potentially regenerative cells. These developments are ushering in a new paradigm of ‘multiphasic’ scaffold designs in which the optimal micro-environment for each tissue region is individually crafted. Although the adoption of these techniques provides new opportunities in OC research, it also introduces challenges, such as creating tissue interfaces, integrating multiple fabrication techniques and co-culturing different cells within the same construct. This review captures the considerations and capabilities in developing 3D printed OC scaffolds, including materials, fabrication techniques, mechanical function, biological components and design.

show abstract

Cell compaction influences the regenerative potential of passaged bovine articular chondrocytes in an ex vivo cartilage defect model

Cited by 8 publications

References 30 publications

Development of an Ex Vivo Murine Osteochondral Repair Model

Development of an Ex Vivo Murine Osteochondral Repair Model

Insights into the present and future of cartilage regeneration and joint repair

3D Printed Multiphasic Scaffolds for Osteochondral Repair: Challenges and Opportunities

Contact Info

Product

Resources

About