Cell chip with a thiolated chitosan self-assembled monolayer to detect the effects of anticancer drugs on breast normal and cancer cells

Ko, Eun-Bi; Cho, Hyeon-Yeol; Kim, Tae-Hyung; Yea, Cheol-Heon; Choi, Jeong‐Woo

doi:10.1016/j.colsurfb.2013.08.016

Cited by 16 publications

(6 citation statements)

References 21 publications

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“…Typical chitosan modification with thiol groups includes covalent attachment between the amine group and the mercaptocarboxylic acid by means of reaction intermediate from carbodiimide (EDAC) and Nhydroxysuccinimide (NHS) [24][25][26][27]. For instance, Ko et al [28] synthesized a novel thiolated chitosan by coupling reaction of 3-mercaptopropionic acid, LMWC/EDAC complex.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Characterization of New Thiolated Chitosan Nanoparticles Obtained by Ionic Gelation Method

Esquivel

Juárez

Almada

et al. 2015

International Journal of Polymer Science

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We derivatized low molecular weight chitosan (LMWC) with 3-mercaptopropanoic acid (3-MPA) by a coupling reaction. The chemical modification of LMWC was characterized by Fourier transform infrared spectroscopy (FT-IR) and nuclear magnetic resonance,1HNMR. We researched the influence of 3-MPA on the nanoparticles formation by ionic gelation method using sodium tripolyphosphate (TPP) as cross-linker reagent. In order to optimize the nanoparticles formation, we studied the effect of the pH solution and molar ratio on nanoparticles stability. Analyses of particle size, morphology, and surface charge were determined by dynamic light scattering, Atomic Force Microscopy, and zeta potential, respectively. It was found that formation of semispherical and stable nanoparticles was improved due to the chemical modification of chitosan. Optimized semispherical nanoparticles of thiolated chitosan were synthesized with the parameters (pH 4.7, molar ratios 1 : 106). Additionally, we reported the thermodynamic profile of the nanoparticles formation determined by isothermal titration calorimetry (ITC). The aggregation process achieved to form nanoparticles of thiolated and nonmodified chitosan consisted of two stages, considering one binding site model. Gibbs free energy(ΔG)and binding constant (Ka) describe the aggregation process of thiolated chitosan/TPP, which is an initial reaction and followed by an endothermic stage. These results are promising for the possible application of these nanoparticles as nanocarriers and delivery systems.

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Section: Introductionmentioning

confidence: 99%

Synthesis and Characterization of New Thiolated Chitosan Nanoparticles Obtained by Ionic Gelation Method

Esquivel

Juárez

Almada

et al. 2015

International Journal of Polymer Science

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“…Therefore, DPV is more sensitive than trypan blue and MTT assays for detecting the cytotoxicity of QDs and capped QDs, particularly at low concentrations (1–10 μg mL −1 ) [ 40 ]. Ko et al reported the development of a cell chip composed of a thiolated chitosan monolayer modified Au electrode that enhanced cell adhesion and electrochemical signals [ 71 ]. This cell chip was applied to distinguish between normal (HMEC) and breast cancer (MCF-7) cells of the same origin; in addition, this cell chip was successfully applied to screen the effects of two anti-cancer drugs (doxorubicin and cyclophosphamide) based on a CV technique.…”

Section: Electrochemical Cell Chipmentioning

confidence: 99%

Nanostructured surfaces for analysis of anticancer drug and cell diagnosis based on electrochemical and SERS tools

2018

Self Cite

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Discovering new anticancer drugs and screening their efficacy requires a huge amount of resources and time-consuming processes. The development of fast, sensitive, and nondestructive methods for the in vitro and in vivo detection of anticancer drugs’ effects and action mechanisms have been done to reduce the time and resources required to discover new anticancer drugs. For the in vitro and in vivo detection of the efficiency, distribution, and action mechanism of anticancer drugs, the applications of electrochemical techniques such as electrochemical cell chips and optical techniques such as surface-enhanced Raman spectroscopy (SERS) have been developed based on the nanostructured surface. Research focused on electrochemical cell chips and the SERS technique have been reviewed here; electrochemical cell chips based on nanostructured surfaces have been developed for the in vitro detection of cell viability and the evaluation of the effects of anticancer drugs, which showed the high capability to evaluate the cytotoxic effects of several chemicals at low concentrations. SERS technique based on the nanostructured surface have been used as label-free, simple, and nondestructive techniques for the in vitro and in vivo monitoring of the distribution, mechanism, and metabolism of different anticancer drugs at the cellular level. The use of electrochemical cell chips and the SERS technique based on the nanostructured surface should be good tools to detect the effects and action mechanisms of anticancer drugs.

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“…Thus, it is less destructive to cells and is bio-degradable in body system. Currently, thiol functionalized chitosan has been successfully developed and used as gene delivery [12], sensitive materials [13], and bio-detection [14,15], etc.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Biosensor Based on Nanocomposites Film of Thiol Graphene‐Thiol Chitosan/Nano Gold for the Detection of Carcinoembryonic Antigen

Li¹,

Xue²,

Feng³

et al. 2015

Electroanalysis

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In this paper, a thiol graphene‐thiol chitosan‐gold nanoparticles (thGP‐thCTS‐AuNPs) nanocomposites film with porous structure was fabricated by electrochemically depositing on glassy carbon electrode (GCE), which exhibited good biocompatibility and improved conductivity, to construct immunosensor free label for detection of carcinoembryonic antigen (CEA). The electrochemical behavior of this immunosensor was investigated by cyclic voltammetry. Under the optimum conditions, the immunosensor revealed a good amperometric response to CEA in two linear ranges (0.3–8.0 ng mL−1 and 8.0–100 ng mL−1) with a detection limit of 0.03 ng mL−1. The results indicated that the immunosensor has the advantages of good selectivity, high sensitivity, and good stability for the determination of CEA.

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Cell chip with a thiolated chitosan self-assembled monolayer to detect the effects of anticancer drugs on breast normal and cancer cells

Cited by 16 publications

References 21 publications

Synthesis and Characterization of New Thiolated Chitosan Nanoparticles Obtained by Ionic Gelation Method

Synthesis and Characterization of New Thiolated Chitosan Nanoparticles Obtained by Ionic Gelation Method

Nanostructured surfaces for analysis of anticancer drug and cell diagnosis based on electrochemical and SERS tools

Electrochemical Biosensor Based on Nanocomposites Film of Thiol Graphene‐Thiol Chitosan/Nano Gold for the Detection of Carcinoembryonic Antigen

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