Cell–cell signaling during the Brassicaceae self-incompatibility response

Abhinandan, Kumar; Sankaranarayanan, Subramanian; MacGregor, Stuart; Goring, Daphne R.; Samuel, Marcus A.

doi:10.1016/j.tplants.2021.10.011

Cited by 34 publications

(41 citation statements)

References 96 publications

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“…Many studies have reported the active role of lectin receptor kinase in pollen development, pollen interaction with stigma, pollen rupture in the embryo sac, pollen tube, and ovule interaction and response to environmental stimuli [ 38 , 39 ]. In Arabidopsis , the expression of lectin receptor kinase was shown to influence pollen development, pollen sterility, and microsporogenesis [ 40 , 41 ]. Significantly, G-type lectin S-receptor-like serine/threonine-protein kinase was highly induced in the tolerant genotype (Ca17969; FC: 6.77 ↑) but repressed in the sensitive (Ca17969; FC: −1.16 ↓).…”

Section: Resultsmentioning

confidence: 99%

Comparative Flower Transcriptome Network Analysis Reveals DEGs Involved in Chickpea Reproductive Success during Salinity

Kaashyap

Ford

Mann

et al. 2022

Plants

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Salinity is increasingly becoming a significant problem for the most important yet intrinsically salt-sensitive grain legume chickpea. Chickpea is extremely sensitive to salinity during the reproductive phase. Therefore, it is essential to understand the molecular mechanisms by comparing the transcriptomic dynamics between the two contrasting genotypes in response to salt stress. Chickpea exhibits considerable genetic variation amongst improved cultivars, which show better yields in saline conditions but still need to be enhanced for sustainable crop production. Based on previous extensive multi-location physiological screening, two identified genotypes, JG11 (salt-tolerant) and ICCV2 (salt-sensitive), were subjected to salt stress to evaluate their phenological and transcriptional responses. RNA-Sequencing is a revolutionary tool that allows for comprehensive transcriptome profiling to identify genes and alleles associated with stress tolerance and sensitivity. After the first flowering, the whole flower from stress-tolerant and sensitive genotypes was collected. A total of ~300 million RNA-Seq reads were sequenced, resulting in 2022 differentially expressed genes (DEGs) in response to salt stress. Genes involved in flowering time such as FLOWERING LOCUS T (FT) and pollen development such as ABORTED MICROSPORES (AMS), rho-GTPase, and pollen-receptor kinase were significantly differentially regulated, suggesting their role in salt tolerance. In addition to this, we identify a suite of essential genes such as MYB proteins, MADS-box, and chloride ion channel genes, which are crucial regulators of transcriptional responses to salinity tolerance. The gene set enrichment analysis and functional annotation of these genes in flower development suggest that they can be potential candidates for chickpea crop improvement for salt tolerance.

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Section: Resultsmentioning

confidence: 99%

Comparative Flower Transcriptome Network Analysis Reveals DEGs Involved in Chickpea Reproductive Success during Salinity

Kaashyap

Ford

Mann

et al. 2022

Plants

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“…This is followed by the germination of the pollen tube which penetrates the stigmatic cell wall and travels down the reproductive tract until it reaches an unfertilized ovule for fertilization (Hafidh and Honys, 2021, Robichaux and Wallace, 2021). In self-incompatible (SI) Brassicaeae species, the stigma blocks self-incompatible pollen by preventing pollen hydration and germination (Abhinandan et al ., 2022, Goring et al ., 2023). Brassicaceae SI is genetically controlled by two S -locus linked genes encoding the pollen S Cysteine-Rich/ S-locus Protein 11 (SCR/SP11) pepde (Schopfer et al ., 1999, Takayama et al ., 2000, Shiba et al ., 2001) and the stigma S Receptor Kinase (SRK) (Stein et al ., 1991, Goring and Rothstein, 1992, Takasaki et al ., 2000, Silva et al ., 2001).…”

Section: Introductionmentioning

confidence: 99%

Investigating a role forPUB17andPUB16in the self-incompatibility pathway in transgenicArabidopsis thaliana

Beronilla,

Goring

2023

Preprint

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In Brassicaceae self-incompatibility (SI), self-pollen rejection is initiated by the S-haplotype specific interactions between the pollen SCR/SP11 ligand and the stigma S Receptor kinase (SRK). In Brassica SI, a member of the Plant U-Box (PUB) E3 ubiquitin ligases, ARC1, is then activated by SRK in this stigma and cellular events downstream of this cause SI pollen rejection by inhibiting pollen hydration and pollen tube growth. During the transition to selfing, Arabidopsis thaliana lost the SI components, SCR, SRK, and ARC1. However, this trait can be reintroduced into A. thaliana by adding back functional copies of these genes from closely related SI species. Both SCR and SRK are required for this, though the degree of SI pollen rejection varies between accessions, and ARC1 is not always needed to produce a strong SI response. For A. thaliana C24, only transforming with A. lyrata SCR and SRK confers a strong SI trait, and so here we investigated if ARC1-related PUBs were involved in the SI pathway. Two close ARC1 paralogs, PUB17 and PUB16, were selected, and CRISPR/Cas9 technology was used to generate pub17 and pub16 mutations in the C24 accession. These mutants were then crossed into a transgenic A. thaliana SI-C24 line and their potential impact on SI pollen rejection was investigated. Overall, we did not observe any significant differences to implicate PUB17 and PUB16 functioning in the transgenic A. thaliana SI-C24 stigma to reject SI pollen.

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“…The process leading to fertilization in flowering plants begins with the deposition of pollen, the male gametophyte, on the stigma, the receptive part of the female pistil. A general property of Brassicaceae pistils, including Arabidopsis , is the presence of dry stigmas which possess cellular signalling pathways for selective pollen hydration (reviewed in Abhinandan et al., 2022 ). Brassicaceae pollen grains carry a pollen coat on the surface with a number of small Pollen Coat Proteins (PCPs) that can serve as signalling peptides ( Doughty et al., 1993 ; Wang et al., 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Finding new Arabidopsis receptor kinases that regulate compatible pollen-pistil interactions

2022

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Successful fertilization of a flowering plant requires tightly controlled cell-to-cell communication between the male pollen grain and the female pistil. Throughout Arabidopsis pollen-pistil interactions, ligand-receptor kinase signaling is utilized to mediate various checkpoints to promote compatible interactions. In Arabidopsis, the later stages of pollen tube growth, ovular guidance and reception in the pistil have been intensively studied, and thus the receptor kinases and the respective ligands in these stages are quite well understood. However, the components of the earlier stages, responsible for recognizing compatible pollen grains and pollen tubes in the upper reproductive tract are less clear. Recently, predicted receptor kinases have been implicated in the initial stages of regulating pollen hydration and supporting pollen tube growth through the upper regions of the reproductive tract in the pistil. The discovery of these additional signaling proteins at the earlier stages of pollen-pistil interactions has further elucidated the mechanisms that Arabidopsis employs to support compatible pollen. Despite these advances, many questions remain regarding their specific functions. Here, we review the roles of the different receptor kinases, integrate their proposed functions into a model covering all stages of pollen-pistil interactions, and discuss what remains elusive with regard to their functions, respective binding partners and signaling pathways.

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Cell–cell signaling during the Brassicaceae self-incompatibility response

Cited by 34 publications

References 96 publications

Comparative Flower Transcriptome Network Analysis Reveals DEGs Involved in Chickpea Reproductive Success during Salinity

Comparative Flower Transcriptome Network Analysis Reveals DEGs Involved in Chickpea Reproductive Success during Salinity

Investigating a role forPUB17andPUB16in the self-incompatibility pathway in transgenicArabidopsis thaliana

Finding new Arabidopsis receptor kinases that regulate compatible pollen-pistil interactions

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