Cell-cell contacts

Pèlegrin, André; Bongrand, Pierre

doi:10.1007/978-3-642-74471-6_15

Cited by 3 publications

(2 citation statements)

References 56 publications

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“…About 10-fold higher rates of bond formation were obtained with (a) substratum-bound cells: parameter k, was 0.75 s51, and (b) cells resuming their movement after a brief stop. These differences are consistent with the hypothesis that bond formation may be easier before and immediately after cell stop because ofa suitable reorganization of repeller elements present in the cell coat ( 15,16,38). This reorganization might involve time-dependent compression of polymeric molecules or diffusion out of contact areas.…”

Section: Is the Estimated Bond Lifetime Consistent With Currently Avasupporting

confidence: 88%

“…Adhesive efficiency is clearly dependent on numerous parameters such as the wall shear rate, the area ofcell-toendothelium contact, density, mobility and affinity of Address correspondence to P. Bongrand. ligand molecules, and presence of potentially repulsive elements present in the endothelium or leukocyte glycocalix (13)(14)(15)(16). There is thus a need for quantitative models allowing both integration of experimental data and prediction of the outcome of cell-cell contact in various circumstances.…”

Section: Introductionmentioning

confidence: 99%

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Granulocyte-endothelium initial adhesion. Analysis of transient binding events mediated by E-selectin in a laminar shear flow

Kaplanski

Farnarier

Tissot

et al. 1993

Biophysical Journal

176

171

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The adhesion of moving cells to receptor-bearing surfaces is a key step to many important biological processes. Attachment was subjected to extensive modeling. However, the numerical values of kinetic bonding parameters relevant to realistic models of cell adhesion remain poorly known. In this report, we describe the motion of human granulocytes to interleukin-1-activated endothelial cells in presence of a low hydrodynamic drag (a few piconewtons) estimated to be much weaker than a standard ligand-receptor bond. It was thus expected to visualize the formation and rupture of individual bonds. We observed multiple short-time cell arrests with a median duration of 2.43 s. Stop frequency, not duration, was significantly inhibited by anti-E-selectin antibodies. Binding efficiency exhibited an almost linear relationship with the inverse of cell velocity. The distribution of arrest duration was determined: results were consistent with the view that these arrests reflected the formation/dissociation of single ligand-receptor bonds with a spontaneous dissociation rate of 0.5 s-1. The rate of bond formation was on the order of 0.04 s-1 when cells were freely rolling (mean velocity: 19 microns/s) and it exhibited an approximately 10-fold increase after the formation of a first adhesion.

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Section: Is the Estimated Bond Lifetime Consistent With Currently Avasupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Granulocyte-endothelium initial adhesion. Analysis of transient binding events mediated by E-selectin in a laminar shear flow

Kaplanski

Farnarier

Tissot

et al. 1993

Biophysical Journal

176

171

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A node-based version of the cellular Potts model

Scianna

Preziosi

2016

Computers in Biology and Medicine

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The cellular Potts model (CPM) is a lattice-based Monte Carlo method that uses an energetic formalism to describe the phenomenological mechanisms underlying the biophysical problem of interest. We here propose a CPM-derived framework that relies on a node-based representation of cell-scale elements. This feature has relevant consequences on the overall simulation environment. First, our model can be implemented on any given domain, provided a proper discretization (which can be regular or irregular, fixed or time evolving). Then, it allowed an explicit representation of cell membranes, whose displacements realistically result in cell movement. Finally, our node-based approach can be easily interfaced with continuous mechanics or fluid dynamics models. The proposed computational environment is here applied to some simple biological phenomena, such as cell sorting and chemotactic migration, also in order to achieve an analysis of the performance of the underlying algorithm. This work is finally equipped with a critical comparison between the advantages and disadvantages of our model with respect to the traditional CPM and to some similar vertex-based approaches.

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Blood-Brain Barrier Breakdown and Blood-Brain Communication in Neurological and Psychiatric Diseases

Friedman

Kaufer²

2011

Cardiovascular Psychiatry and Neurology

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Cell-cell contacts

Cited by 3 publications

References 56 publications

Granulocyte-endothelium initial adhesion. Analysis of transient binding events mediated by E-selectin in a laminar shear flow

Granulocyte-endothelium initial adhesion. Analysis of transient binding events mediated by E-selectin in a laminar shear flow

A node-based version of the cellular Potts model

Blood-Brain Barrier Breakdown and Blood-Brain Communication in Neurological and Psychiatric Diseases

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