Cell-cell contact modulation of myosin organization in the early mouse embryo

Sobel, J. S.

doi:10.1016/0012-1606(83)90212-9

Cited by 63 publications

(28 citation statements)

References 24 publications

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“…Calmodulin also was regionalized in the areas opposite to cell contacts of compacted morulae. The same location has been reported for myosin (35,74). Inhibition of calmodulin with TFP induced its delocalization whereas morulae decompacted and [Ca 2ϩ ] i rose.…”

Section: Discussionsupporting

confidence: 55%

“…During compaction a number of cellular changes occur within the embryo that ensure the subsequent establishment of the trophectoderm (26)(27)(28). These changes include the formation of tight junctions (29,30), gap junctions (31,32), and cytoskeletal connections (33) between blastomeres, as well as regionalization of different cytoskeletal components and enzymes at the cell contacts generating spatial heterogenity and polarity of the embryo (34)(35)(36)(37)(38)(39). Furthermore, it has been demonstrated that compaction is mediated by microfilaments, microtubules (40)(41)(42)(43), protein kinase C (44), and E-cadherin (2-4), a transmembrane calcium-dependent cell adhesion glycoprotein anchored to the cytoskeleton through catenins (45,46).…”

mentioning

confidence: 99%

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Increase of intracellular Ca ²⁺ and relocation of E-cadherin during experimental decompaction of mouse embryos

Pey

Vial

Schatten

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

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To determine the role of intracellular Ca 2؉ in compaction, the first morphogenetic event in embryogenesis, we analyzed preimplantation mouse embryos under several decompacting conditions, including depletion of extracellular Ca 2؉ , blocking of Ca 2؉ channels, and inhibition of microfilaments, calmodulin, and intracellular Ca 2؉ release. Those treatments induced decompaction of mouse morulae and simultaneously induced changes in cytosolic free Ca 2؉ concentration and deregionalization of E-cadherin and fodrin. When morulae were allowed to recompact, the location of both proteins recovered. In contrast, actin did not change its cortical location with compaction nor with decompaction-recompaction. Calmodulin localized in areas opposite to cell-cell contacts in eight-cell stage embryos before and after compaction. Inhibition of calmodulin with trifluoperazine induced its delocalization while morulae decompacted. A nonspecific rise of intracellular free Ca 2؉ provoked by ionomycin did not affect the compacted shape. Moreover, the same decompacting treatments when applied to uncompacted embryos did not produce any change in intracellular Ca 2؉ . Our results demonstrate that in preimplantation mouse embryos experimentally induced stage-specific changes of cell shape are accompanied by changes of intracellular free Ca 2؉ and redistribution of the cytoskeleton-related proteins E-cadherin, fodrin, and calmodulin. We conclude that intracellular Ca 2؉ specifically is involved in compaction and probably regulates the function and localization of cytoskeleton elements.Extracellular calcium is crucial during the preimplantation development of mammalian embryos because of its role in cell-cell adhesion (1-5). Intracellular free calcium ([Ca 2ϩ ] i ) plays a pivotal role in triggering early events related to fertilization, namely, oocyte maturation, cortical granule exocytosis, and egg activation (6-19). However, little is known about the role of [Ca 2ϩ ] i during the following steps of mammalian development. Indirect evidence for a regulatory role was provided in experiments where development of mouse embryos was delayed by treatments with calcium channel blockers or with an inhibitor of calmodulin (20). It was only recently that [Ca 2ϩ ] i changes were studied directly during early mouse development. An endogenous increase in Ca 2ϩ -releasing activity leading to Ca 2ϩ transients was observed during the first mitotic division (21). Moreover, the rate of cavitation and cell division was either accelerated or delayed by experimentally induced elevation or reduction of [Ca 2ϩ ] i , respectively (22-24). Stacheki and Armant (24) suggested that in mouse embryos, inositol 1,4,5-trisphosphate-sensitive and ryanodinesensitive Ca 2ϩ stores exist at the morula stage and that calmodulin is involved in mediating the Ca 2ϩ signaling effects to its distal targets.Despite these temporal effects, no other developmental events have been examined so far under conditions where [Ca 2ϩ ] i has been manipulated and therefore t...

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Section: Discussionsupporting

confidence: 55%

mentioning

confidence: 99%

Increase of intracellular Ca ²⁺ and relocation of E-cadherin during experimental decompaction of mouse embryos

Pey

Vial

Schatten

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

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“…It is known that there are many factors such as calcium ions (Ducibella & Anderson, 1975), microtubules (Maro & Pickering, 1984), myosin (Sobel, 1983) and protein kinase C (Bloom, 1989) influencing the cell-cell contacts during compaction. Ducibella & Anderson (1975) demonstrated that the calcium ion threshold for compaction is approximately 0-1-0-2 mM and may be required for normal intercellular adhesions.…”

Section: Discussionmentioning

confidence: 99%

Morphology and proportion of inner cell mass of bovine blastocysts fertilized in vitro and in vivo

Iwasaki¹,

Yoshiba²,

Ushijima³

et al. 1990

Reproduction

198

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Summary. The morphology and proportion of inner cell mass (ICM) of bovine blastocysts cultured in vitro or in vivo in rabbit oviducts after in-vitro fertilization of in-vitro matured follicular oocytes were compared with those of blastocysts fertilized in vivo by a differential fluorochrome staining technique. The delineation of each ICM cell was improved by the transfer of embryos derived from in-vitro fertilization to a rabbit oviduct although the cell\p=n-\cellcontacts of ICM cells were not as tight as those from in-vivo fertilization. The proportions (15\m=.\8 and 14\m=.\9%) of ICM in blastocysts cultured in vitro at early and expanded stages were significantly lower than those cultured in rabbit oviducts after in-vitro fertilization and fertilized in vivo. These results show that the transfer of bovine embryos derived from in-vitro fertilization to the rabbit oviduct increased the proliferation of ICM cells to the level of embryos fertilized in vivo although the cell\p=n-\cellcontact of ICM cell is not improved by the process.

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“…The opposite effect is seen in embryos of the crustacean Sicyonia; in this case, spindle poles are repelled by cell contact zones, apparently independent of cell shape (Wang et al, 1997). In early mouse embryos, cell contacts have been shown to reduce the accumulation of cortical myosin locally (Sobel, 1983), suggesting a possible molecular mechanism for cleavage plane positioning in Ilyanassa.…”

Section: Control Of Cell Division Geometry By Intercellular Contactsmentioning

confidence: 99%

Cell contact-dependent positioning of the D cleavage plane restricts eye development in theIlyanassaembryo

Goulding

2003

Development

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In embryos of the gastropod Ilyanassa obsoleta, the first-quartet micromeres of the A, B and C lineages (1a, 1b, and 1c) are each competent to form an eye in response to signaling from the 3D cell. The first-quartet micromere of the dorsal D lineage (1d) is smaller than the others, divides at a slower rate, and lacks the ability to form an eye. These properties of 1d all depend on inheritance of vegetal polar lobe cytoplasm by its mother cell D at second cleavage. I show that they depend also on the presence of cells adjacent to D during the late four-cell stage: after ablation of the A and/or C cells before this stage, 1d inherits more cytoplasm than normal, divides more rapidly, and frequently forms an eye. In non-D lineages, cleavage plane positioning and micromere division rates are relatively insensitive to cell contacts. Compressing whole embryos during third cleavage also leads to an increase in 1d volume correlated with abnormal eye formation; this suggests that the normal effect of cell contacts is to position the D cell cleavage furrow closer to the animal pole, and the enhanced division asymmetry of the D cell contributes to the suppression of eye development.

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Cell-cell contact modulation of myosin organization in the early mouse embryo

Cited by 63 publications

References 24 publications

Increase of intracellular Ca ²⁺ and relocation of E-cadherin during experimental decompaction of mouse embryos

Increase of intracellular Ca ²⁺ and relocation of E-cadherin during experimental decompaction of mouse embryos

Morphology and proportion of inner cell mass of bovine blastocysts fertilized in vitro and in vivo

Cell contact-dependent positioning of the D cleavage plane restricts eye development in theIlyanassaembryo

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Cell-cell contact modulation of myosin organization in the early mouse embryo

Cited by 63 publications

References 24 publications

Increase of intracellular Ca 2+ and relocation of E-cadherin during experimental decompaction of mouse embryos

Increase of intracellular Ca 2+ and relocation of E-cadherin during experimental decompaction of mouse embryos

Morphology and proportion of inner cell mass of bovine blastocysts fertilized in vitro and in vivo

Cell contact-dependent positioning of the D cleavage plane restricts eye development in theIlyanassaembryo

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Increase of intracellular Ca ²⁺ and relocation of E-cadherin during experimental decompaction of mouse embryos

Increase of intracellular Ca ²⁺ and relocation of E-cadherin during experimental decompaction of mouse embryos