Cell-bound exopolysaccharides of <i>Lactobacillus brevis </i>
KB290 enhance cytotoxic activity of mouse splenocytes

Sasaki, Erika; Suzuki, Shigenori; Fukui, Yuichiro; Yajima, Nobuhiro

doi:10.1111/jam.12686

Cited by 14 publications

(9 citation statements)

References 33 publications

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“…It was reported that the thermal treatments increase the cell coarseness and roughness, which influence their immune-modulating properties [6,7]. In addition, heat-treated probiotics not only possess biological activities in communication with the host but also provide advantages for industrial production and consumer usage, including a longer product shelf life, easier storage, and more convenient transportation [4,6,8].…”

Section: Introductionmentioning

confidence: 99%

Micronized and Heat-Treated Lactobacillus plantarum LM1004 Stimulates Host Immune Responses Via the TLR-2/MAPK/NF-��B Signalling Pathway In Vitro and In Vivo

Lee¹,

Jung²,

Choi³

et al. 2019

Journal of Microbiology and Biotechnology

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Although nanometric dead Lactobacillus plantarum has emerged as a potentially important modulator of immune responses, its underlying mechanism of action has not been fully understood. This study aimed to identify the detailed biochemical mechanism of immune modulation by micronized and heat-treated L. plantarum LM1004 (MHT-LM1004, <1 μm in size). MHT-LM1004 was prepared from L. plantarum LM1004 via culture in a specifically designed membrane bioreactor and heat treatment. MHT-LM1004 was shown to effectively induce the secretion of TNF-α and IL-6 and the mRNA expression of inducible nitric oxide synthase (iNOS). MHT-LM1004 enhanced the expression of TLR-2, phosphorylation of MAPKs (ERK), and nuclear translocation of NF-κB in a dose-dependent manner. Oral administration of MHT-LM1004 (4 × 10 9 or 4 × 10 1 1 cells/kg mouse body weight) increased the splenocyte proliferation and serum cytokine levels. These results suggested that MHT-LM1004 effectively enhances early innate immunity by activating macrophages via the TLR-2/MAPK/NF-κB signalling pathway and that this pathway is one of the major routes in immune modulation by the Lactobacillus species.

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Section: Introductionmentioning

confidence: 99%

Micronized and Heat-Treated Lactobacillus plantarum LM1004 Stimulates Host Immune Responses Via the TLR-2/MAPK/NF-��B Signalling Pathway In Vitro and In Vivo

Lee¹,

Jung²,

Choi³

et al. 2019

Journal of Microbiology and Biotechnology

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“…First, this trial has a lack of information on the mechanism of KB290 + βC against influenza, as we did not measure immunological parameters such as innate immune responses or antibodies in this trial. Our previous studies have shown that live KB290 induced the production of interferon-α and immunoglobulin A in mice challenged with IAV [ 36 ] and enhanced cell-mediated cytotoxic activity in mice [ 37 ]. In addition to the immunoregulatory actions of βC [ 38 ], retinoic acid has also been reported to be associated with retinoic acid-inducible gene-I-mediated production of interferon-α and immunoglobulin A [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of Heat-Killed Levilactobacillus brevis KB290 in Combination with β-Carotene on Influenza Virus Infection in Healthy Adults: A Randomized Controlled Trial

Satomi¹,

Waki²,

Arakawa³

et al. 2021

Nutrients

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Influenza, a seasonal acute respiratory disease caused primarily by the influenza virus A or B, manifests with severe symptoms leading to considerable morbidity and mortality and is a major concern worldwide. Therefore, effective preventive measures against it are required. The aim of this trial was to evaluate the preventive effects of heat-killed Levilactobacillus brevis KB290 (KB290) in combination with β-carotene (βC) on influenza virus infections in healthy Japanese subjects aged between 20 and 59 y throughout the winter season. We performed a randomized, double-blind, placebo-controlled, parallel-group trial from 16 December 2019 to 8 March 2020, comparing KB290 + βC beverage with placebo beverage. The primary endpoint was the incidence of influenza based on a doctor’s certificate. The incidence of influenza was not significantly different between the two groups. However, the subgroup analysis showed a significant difference between the two groups (influenza incidence: the KB290 + βC group 1.9%, and the placebo group 3.9%) in the subgroup of subjects aged ˂40 y, but not in the subgroup of subjects aged ≥40 y. The results of this trial suggest that the combination of KB290 and βC might be a possible candidate supplement for protection against the seasonal influenza virus infection in humans aged <40 y, although further clinical studies are needed to confirm the concrete preventive effect of this combination on influenza.

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“…This finding was in agreement with a previous report, which demonstrated that live KB290 can reduce the influence of IAV in mice [ 14 ]. In addition, both live and heat-killed KB290 have been shown to enhance cell-mediated cytotoxic activity in mice [ 66 ]. Taken together, these findings suggest that both live and heat-killed forms of KB290, could be effective in providing protection against IAV infection by possibly enhancing the function of immune cells.…”

Section: Discussionmentioning

confidence: 99%

Short Communication: Oral Administration of Heat-killed Lactobacillus brevis KB290 in Combination with Retinoic Acid Provides Protection against Influenza Virus Infection in Mice

Satomi¹,

Khanum

Miller

et al. 2020

Nutrients

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Influenza virus type A (IAV) is a seasonal acute respiratory disease virus with severe symptoms, and an effective preventive measure is required. Despite many reports describing the potentially protective effects of lactic acid bacteria, few studies have investigated the effects of nutritional supplement combinations. This study reports the effect of the combined intake of heat-killed Lactobacillus brevis KB290 (KB290) and vitamin A (VA) on mice challenged with a sublethal dose of IAV. For 2 weeks, five groups of mice were fed either placebo, KB290, VA, or a combination of KB290 and VA (KB290+VA). After subsequent IAV challenge, bodyweight and general health were monitored for up to 2 weeks. Viral titres were determined in the lungs of animal subgroups euthanised at days 3, 7, and 14 after IAV challenge. A significant loss was observed in the bodyweights of IAV-infected animals from day 1 post-IAV challenge, whereas the mice fed KB290+VA did not lose any weight after IAV infection, indicating successful protection from the infection. Additionally, mice in the KB290+VA group showed the highest reduction in lung viral titres. In conclusion, the combination of KB290 and VA could be a useful food supplement relevant for protection against seasonal influenza virus infection in humans.

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Cell-bound exopolysaccharides of Lactobacillus brevis KB290 enhance cytotoxic activity of mouse splenocytes

Abstract: We demonstrated that EPS-b produced by KB290 has a critical role in enhancing cell-mediated cytotoxic activity in mouse spleen.

Cited by 14 publications

References 33 publications

Micronized and Heat-Treated Lactobacillus plantarum LM1004 Stimulates Host Immune Responses Via the TLR-2/MAPK/NF-��B Signalling Pathway In Vitro and In Vivo

Micronized and Heat-Treated Lactobacillus plantarum LM1004 Stimulates Host Immune Responses Via the TLR-2/MAPK/NF-��B Signalling Pathway In Vitro and In Vivo

Effects of Heat-Killed Levilactobacillus brevis KB290 in Combination with β-Carotene on Influenza Virus Infection in Healthy Adults: A Randomized Controlled Trial

Short Communication: Oral Administration of Heat-killed Lactobacillus brevis KB290 in Combination with Retinoic Acid Provides Protection against Influenza Virus Infection in Mice

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