Cell block samples from malignant pleural effusion might be valid alternative samples for anaplastic lymphoma kinase detection in patients with advanced non‐small‐cell lung cancer

Zhou, Jianya; Yao, Huaiying; Zhao, Jingbo; Zhang, Shumeng; Qin, You; Sun, Ke; Zou, Yinying; Zhou, Caicun

doi:10.1111/his.12560

Cited by 31 publications

(51 citation statements)

References 18 publications

(39 reference statements)

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“…FISH using fresh pleural fluid samples should be considered as an additional informative assay for pleural effusions suspicious of being malignant [40, 41]. Furthermore, cell block preparations are commonly used for diagnostic pathology and gene analysis by FISH and IHC [42, 43]. A few reports reported that compared with tumor tissue, pleural effusion cell block had 81.8% sensitivity and 80% specificity for detection of EGFR mutation [44] and 62.5% to 100% sensitivity and 100% specificity for ALK detection [43].…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, cell block preparations are commonly used for diagnostic pathology and gene analysis by FISH and IHC [42, 43]. A few reports reported that compared with tumor tissue, pleural effusion cell block had 81.8% sensitivity and 80% specificity for detection of EGFR mutation [44] and 62.5% to 100% sensitivity and 100% specificity for ALK detection [43]. For successful detection of the targeted gene, sufficient amount of malignant cells are needed; however, in some cases, like our patient (Fig 1), the amount of malignant cells in cell block samples are few.…”

Section: Discussionmentioning

confidence: 99%

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Diagnostic Utility of Pleural Fluid Cell Block versus Pleural Biopsy Collected by Flex-Rigid Pleuroscopy for Malignant Pleural Disease: A Single Center Retrospective Analysis

et al. 2016

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BackgroundSome trials recently demonstrated the benefit of targeted treatment for malignant disease; therefore, adequate tissues are needed to detect the targeted gene. Pleural biopsy using flex-rigid pleuroscopy and pleural effusion cell block analysis are both useful for diagnosis of malignancy and obtaining adequate samples. The purpose of our study was to compare the diagnostic utility between the two methods among patients with malignant pleural disease with effusion.MethodsData from patients who underwent flex-rigid pleuroscopy for diagnosis of pleural effusion suspicious for malignancy at the National Cancer Center Hospital, Japan between April 2011 and June 2014 were retrospectively reviewed. All procedures were performed under local anesthesia. At least 150 mL of pleural fluid was collected by pleuroscopy, followed by pleural biopsies from the abnormal site.ResultsThirty-five patients who were finally diagnosed as malignant pleural disease were included in this study. Final diagnoses of malignancy were 24 adenocarcinoma, 1 combined adeno-small cell carcinoma, and 7 malignant pleural mesothelioma (MPM), and 3 metastatic breast cancer. The diagnostic yield was significantly higher by pleural biopsy than by cell block [94.2% (33/35) vs. 71.4% (25/35); p = 0.008]. All patients with positive results on cell block also had positive results on pleural biopsy. Eight patients with negative results on cell block had positive results on pleural biopsy (lung adenocarcinoma in 4, sarcomatoid MPM in 3, and metastatic breast cancer in 1). Two patients with negative results on both cell block and pleural biopsy were diagnosed was sarcomatoid MPM by computed tomography-guided needle biopsy and epithelioid MPM by autopsy.ConclusionPleural biopsy using flex-rigid pleuroscopy was efficient in the diagnosis of malignant pleural diseases. Flex-rigid pleuroscopy with pleural biopsy and pleural effusion cell block analysis should be considered as the initial diagnostic approach for malignant pleural diseases presenting with effusion.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Diagnostic Utility of Pleural Fluid Cell Block versus Pleural Biopsy Collected by Flex-Rigid Pleuroscopy for Malignant Pleural Disease: A Single Center Retrospective Analysis

et al. 2016

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“…Activated ALK could inhibit apoptosis and promote the proliferation through activation of down-stream PI3K/Akt and MAPK signalling pathways [17]. Accumulating evidence suggests that ALK was increased in a variety of tumors, including lung cancer [18], neuroblastoma [19], pancreatic cancer [20] and glioblastoma [21]. Martinengo et al found that under hypoxic conditions ALK directly regulated the abundance of hypoxia-inducible factors (HIFs) in both anaplastic large cell lymphoma and non-small cell lung carcinoma.…”

Section: Discussionmentioning

confidence: 99%

miR-1271 inhibits OSCC cell growth and metastasis by targeting ALK

Kong¹,

Zhang²,

Ma³

et al. 2015

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Aberrant expression of miR-1271 plays prominent roles in progression of several cancers. However, the role of miR-1271 in oral squamous cell carcinoma (OSCC) has not been elucidated. The expression levels of miR-1271 and anaplastic lymphoma kinase (ALK) were measured by qRT-PCR in OSCC tissues and cell lines. The effects of miR-1271-mediated ALK on growth and metastasis of OSCC cells, transfected with miR-1271 with or without ALK plasmid, were assessed using MTT, colony formation, migration and invasion assays. Moreover, the target relationship between miR-1271 and ALK was assessed using luciferase reporter assay, western blotting and qRT-PCR. We found that miR-1271 was significantly decreased in OSCC tissues and cell lines. Overexpression of miR-1271 suppressed cell proliferation, colony formation, migration and invasion of OSCC cells. Furthermore, ALK was identified as a target of miR-1271, ALK overexpression remarkably attenuated the tumor suppressive effects of miR-1271 on OSCC cells. In addition, ALK was inversely correlated with miR-1271 expression in OSCC tissues. Taken together, our findings indicate that miR-1271 acts as tumor suppressor in OSCC and might be used as an therapeutic target for the development of treatment for OSCC.

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“…Regarding ALK genetic analysis, although fluorescent in-situ hybridization is considered to be the gold standard technique, two recent head-to-head comparison studies showed that RT-PCR [18] and Ventana immunohistochemistry [19] are better candidate methodologies when applied to blocks of pleural effusion cells. ALK rearrangement detection rates were 4.5% (3/66) and 15.4% (8/52), respectively.…”

Section: Key Pointsmentioning

confidence: 99%