Cell biology of gingival wound healing

Häkkinen, Lari; Uitto, Veli‐Jukka; Larjava, Hannu

doi:10.1034/j.1600-0757.2000.2240107.x

Cited by 202 publications

(190 citation statements)

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“…However, dramatically increased numbers of myofibroblasts appear during the wound healing processes [Hakkinen et al, 2000]. It has been found that myofibroblast differentiation occurs in HGFs in vitro [Arora and McCulloch, 1999], and our results confirmed that upon TGF-β1 stimulation HGFs could differentiate into myofibroblasts.…”

Section: Discussionsupporting

confidence: 83%

Nicotine inhibits myofibroblast differentiation in human gingival fibroblasts

Fang

Svoboda

2005

J of Cellular Biochemistry

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Cigarette smoking has been suggested as a risk factor for several periodontal diseases. It has also been found that smokers respond less favorably than non-smokers to periodontal therapy. Previous work in our lab has shown that nicotine inhibits human gingival cell migration. Since myofibroblasts play an important role in wound closure, we asked if nicotine affects gingival wound healing process by regulating myofibroblast differentiation. Human gingival fibroblasts (HGFs) from two patients were cultured in 10% fetal bovine serum cell culture medium. Cells were pretreated with different doses of nicotine (0, 0.01, 0.1, and 1 mM) for 2 h, and then incubated with transforming growth factor beta (TGF-β1) (0, 0.25, 0.5, and 1 ng/ml) with or without nicotine for 30 h. The expression level of α-smooth muscle actin (α-SMA), a specific marker for myofibroblasts, was analyzed by Western blots, immunocytochemistry, and real-time polymerase chain reaction (real-time PCR). Phosphorylated p38 mitogen-activated protein kinase (Phospho-p38 MAPK) activity was analyzed by Western blots. TGF-β1 induced an increase of α-SMA protein and mRNA expression, while nicotine (1 mM) inhibited the TGF-β1-induced expression of α-SMA but not β-actin. Nicotine treatment down-regulated TGF-β1-induced p38 MAPK phosphorylation. Our results demonstrated for the first time that nicotine inhibits myofibroblast differentiation in human gingival fibroblasts in vitro; supporting the hypothesis that delayed wound healing in smokers may be due to decreased wound contraction by myofibroblasts. Keywordshuman gingival fibroblasts; myofibroblasts; α smooth muscle actin; TGFβ; p38 MAP kinase Myofibroblasts are contractile cells that share characteristics of fibroblasts and smooth muscle cells [Tomasek et al., 2002]. One of the major features of myofibroblast differentiation is the expression of smooth muscle cell markers. These markers include α-SMA, γ-SMA, smooth muscle 22 (SM22), calponin, smoothelin, meta-vinculin, h-caldesmon [Halayko and Solway, 2001]. Among these, α-SMA has been suggested as the most reliable marker of myofibroblast differentiation [Gabbiani, 2003]. Also, Dr. Masur's lab demonstrated that myofibroblast differentiation was cell density dependent in corneal fibroblasts. They found that the lower the cell density when cells were seeded in culture, the more cells differentiated into myofibroblasts. However, the myofibroblast phenotype was not terminal. The cells lost the myofibroblast

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Section: Discussionsupporting

confidence: 83%

Nicotine inhibits myofibroblast differentiation in human gingival fibroblasts

Fang

Svoboda

2005

J of Cellular Biochemistry

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“…This new matrix supports the osteogenic cells migration Lang et al (2003), stimulated by several molecules released during blood clotting and clot cleansing, such as PDGF, TGF-β Davies (2003) and fibroblast growth factor (FGF) Dimitriou et al (2005). Between the 7-10th day of healing, some of the fibroblasts present in the interface are transformed into myoblasts Häkkinen et al (2000) characterized by smooth muscle α-actin cytoplasmatic microfilaments, allowing them to generate contractile forces responsible for wound contraction Aukhil (2000) Biological activity regarding wound healing at the bone-dental implant interface concludes with the modeling and subsequent bone remodeling Sikavitsas et al (2001). Moreover, cell adhesion, cell migration and proliferation on surrounding tissues, and internal and external mechanical loads action modify the new tissue formation profile ( Figure 4).…”

Section: Mechanobiology Of the Bone-dental Implant Interfacementioning

confidence: 96%

“…First, the adhesion phenomena produced by cells fixation to a substrate Anselme (2000) activate chemical signaling Kasemo (2002) controlling cell proliferation and differentiation, as in platelet aggregation and activation stages Collen & Lijnen (1991). Then, the contraction exerted by cells moving over a substrate Häkkinen et al (2000) may cause the displacement of the attached fibrin fibers and their detachment from the implant surface Davies (2003;. This kind of event is present during fibroplasia and modeling stages where fibroblasts and osteogenic cells adhere to the fibrin network and begin to move through it in an attempt to colonize the implant surface.…”

Section: Mechanobiology Of the Bone-dental Implant Interfacementioning

confidence: 99%

Mechanobiology of Oral Implantable Devices

Guerrero¹,

Vanegas²,

Garzón³

et al. 2011

Biomaterials Science and Engineering

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“…The difference lies in the gingival wound where keratinocyte migrates directly to fibrinfibronectin matrix without contacting with collagen 26,27 . The wound healing in mouth mucosa is relatively faster, and scraped tissues are seldom forming in this case.…”

Section: Discussionmentioning

confidence: 99%

“…Fibroblast in adult gingival is located in string tissue papilla, and some of its components share with the fibroblast of fetal. There are several factors in saliva, such as growth factor and ion (magnesium and calcium), that function to decrease some material components that may influence the gingival healing 27 . The moisturization of mouth by saliva also functions to help heal wound.…”

Section: Discussionmentioning

confidence: 99%

Augmentation of Demineralized Bone Matrix Post-Tooth Extraction Increases The Density of Gingival Collagen Fiber of Rabbit Mandible

Tandelilin

2010

The Indonesian J Dent Res

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Most cases report that the abnormality of bone defect is engendered by tooth extraction. The powder of demineralized bone matrix (DBM) is required to fill alveolus bone for reconstructing material or preventing tissues defect after tooth extraction. The aim of this study was to determine the density of gingival collagen fiber on wound healing after the augmentation of DBM following the extraction of incisivus tooth. In this study, 36 male rabbits aged 2.5-3 months weighing 900-1,100 grams were randomly divided into two groups: control and treated rabbits. The incisivus teeth of mandibles of treated rabbits were extracted and augmented with the allograft DBM powder. The gingival was sutured with non-absorbable sutures. The same procedures were employed to the control group, except that these rabbits were augmented with DBM powder. Subsequently, the rabbits were sacrificed on days 1, 3, 5, 7, 10, and 14 after surgery, and each observation was represented by three rabbits in the sample. The gingival (ca. 0.5-1cm) was cut and fixed immediately in 10% paraformaldehide. The staining was done using van Geison. In the treated rabbits, the density of gingival collagen fiber significantly increases in all observation days except on first day, indicating that the allograft powder of DBM successfully accelerates the wound healing process.

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Cell biology of gingival wound healing

Cited by 202 publications

References 0 publications

Nicotine inhibits myofibroblast differentiation in human gingival fibroblasts

Nicotine inhibits myofibroblast differentiation in human gingival fibroblasts

Mechanobiology of Oral Implantable Devices

Augmentation of Demineralized Bone Matrix Post-Tooth Extraction Increases The Density of Gingival Collagen Fiber of Rabbit Mandible

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