Cell biology at the interface of nanobiosensors and microfluidics

Bhalla, Nikhil; Chiang, Hung-Ju; Shen, Amy Q.

doi:10.1016/bs.mcb.2018.09.009

Cited by 8 publications

(7 citation statements)

References 93 publications

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“…One promising field is biosensing technology that seeks to combine current conventional methods with lab‐on‐chip devices to facilitate EV analysis. Biosensors are analytical instruments that detect and/or quantify biomarkers by producing signals related to the concentration of an analyte (Bhalla et al, 2018 ). These biosensors are composed of a biological receptor that provides specificity and a transducer that converts biological signals into electrical data (Naresh & Lee, 2021 ).…”

Section: Future Directions For Ev Isolation and Ch...mentioning

confidence: 99%

Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy

Sousa

Rossi

Abdullahi

et al. 2022

WIREs Nanomed Nanobiotechnol

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Section: Future Directions For Ev Isolation and Ch...mentioning

confidence: 99%

Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy

Sousa

Rossi

Abdullahi

et al. 2022

WIREs Nanomed Nanobiotechnol

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“…Typically, the molecular binding at the sensor's surface leads to a refractive index change. 265 For example, Roether et al integrated mushroom-shaped plasmonic nanostructures into a microfluidic channel and monitored the realtime DNA polymerase activity in a label-free manner. 266 Another attractive label-free biosensing technique is the quartz crystal microbalance (QCM), which can sense the mass variation originating from molecular binding.…”

Section: ■ Sample Extraction and Preparationmentioning

confidence: 99%

“…Recently, localized surface plasmon resonance (LSPR) techniques have been developed for label-free biosensing applications. Typically, the molecular binding at the sensor’s surface leads to a refractive index change . For example, Roether et al.…”

Section: Amplification-free Sensingmentioning

confidence: 99%

Challenges and Opportunities for Clustered Regularly Interspaced Short Palindromic Repeats Based Molecular Biosensing

Bao

Chen

et al. 2021

ACS Sens.

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Clustered regularly interspaced short palindromic repeats, CRISPR, has recently emerged as a powerful molecular biosensing tool for nucleic acids and other biomarkers due to its unique properties such as collateral cleavage nature, room temperature reaction conditions, and high target-recognition specificity. Numerous platforms have been developed to leverage the CRISPR assay for ultrasensitive biosensing applications. However, to be considered as a new gold standard, several key challenges for CRISPR molecular biosensing must be addressed. In this paper, we briefly review the history of biosensors, followed by the current status of nucleic acid-based detection methods. We then discuss the current challenges pertaining to CRISPR-based nucleic acid detection, followed by the recent breakthroughs addressing these challenges. We focus upon future advancements required to enable rapid, simple, sensitive, specific, multiplexed, amplification-free, and shelf-stable CRISPR-based molecular biosensors.

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“…These strategies rely on the direct detection of receptor–analyte reactions occurring on recognition elements such as metallic thin films, nanostructures, or field-effect transistors by monitoring localized changes in absorbance wavelengths or electronic transduction proportional to the changes in surface mass adsorption . The most commonly used label-free detection strategies rely on optical sensing of mass dependent surface electron resonance energy changes using surface-enhanced Raman spectroscopy, − surface plasmon resonance, − and localized surface plasmon resonance sensors, − or direct detection of mass variation using quartz crystal microbalance. − In addition to optical detection techniques, high sensitivity field-effect transistors have enabled the development of ultrasensitive electrochemical analyte detection systems with LoDs in the low attomolar to zeptomolar ranges. , …”

Section: Detection Of Receptor–analyte Reactionsmentioning

confidence: 99%

Toward the Development of Rapid, Specific, and Sensitive Microfluidic Sensors: A Comprehensive Device Blueprint

Sathish

Shen

2021

JACS Au

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Recent advances in nano/microfluidics have led to the miniaturization of surface-based chemical and biochemical sensors, with applications ranging from environmental monitoring to disease diagnostics. These systems rely on the detection of analytes flowing in a liquid sample, by exploiting their innate nature to react with specific receptors immobilized on the microchannel walls. The efficiency of these systems is defined by the cumulative effect of analyte detection speed, sensitivity, and specificity. In this perspective, we provide a fresh outlook on the use of important parameters obtained from well-characterized analytical models, by connecting the mass transport and reaction limits with the experimentally attainable limits of analyte detection efficiency. Specifically, we breakdown when and how the operational (e.g., flow rates, channel geometries, mode of detection, etc.) and molecular (e.g., receptor affinity and functionality) variables can be tailored to enhance the analyte detection time, analytical specificity, and sensitivity of the system (i.e., limit of detection). Finally, we present a simple yet cohesive blueprint for the development of high-efficiency surface-based microfluidic sensors for rapid, sensitive, and specific detection of chemical and biochemical analytes, pertinent to a variety of applications.

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Cell biology at the interface of nanobiosensors and microfluidics

Cited by 8 publications

References 93 publications

Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy

Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy

Challenges and Opportunities for Clustered Regularly Interspaced Short Palindromic Repeats Based Molecular Biosensing

Toward the Development of Rapid, Specific, and Sensitive Microfluidic Sensors: A Comprehensive Device Blueprint

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