Cell biology and functional dynamics of the mammalian sperm surface

Gadella, B.M.; Luna, Carolina

doi:10.1016/j.theriogenology.2013.09.005

Cited by 72 publications

(62 citation statements)

References 117 publications

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“…This underscores its functional and maturational roles in sperm. Thus, in the female tract the acquisition of additional PMCA4 from the OVS might be important for maintaining sperm viability during capacitation (the final sperm maturational stage), as well as in hyperactivation, and acrosome reaction (AR) (6), which all require elevated levels of Ca 2ϩ (13)(14)(15)(16).…”

mentioning

confidence: 99%

Oviductosome-Sperm Membrane Interaction in Cargo Delivery

Al‐Dossary

Bathala

Caplan

et al. 2015

Journal of Biological Chemistry

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Background: The mechanism(s) for delivery of transmembrane proteins to sperm via exosomes/microvesicles is unknown. Results: Using SR/SIM and a lipophilic dye, fusion detected for delivery of PMCA4 was inhibited by blocking integrin/ligand interactions. Conclusion: Integrins on sperm and microvesicles play a role in microvesicle fusion for cargo delivery. Significance: Delivery of reproductive microvesicle cargo that modulates fertility is mediated by integrins.

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mentioning

confidence: 99%

Oviductosome-Sperm Membrane Interaction in Cargo Delivery

Al‐Dossary

Bathala

Caplan

et al. 2015

Journal of Biological Chemistry

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“…This last event represents an important step because it promotes the activation of several enzymes that are involved in lipid translocation across specific domains of sperm PM (particularly located in the anterior area of sperm head). Here, the segregation of specific classes of lipids in the inner or outer leaflet of plasma membrane [17,18] has been described. The aminophospholipids, phosphatidylserine (PS) and phosphatidylethanolamine are specifically concentrated in the inner leaflet, while choline phospholipids sphingomyelin and phosphatidylcholine in the outer one.…”

Section: Spermatozoa Membrane Dynamicsmentioning

confidence: 99%

“…In unsaturated fatty acid-rich areas of membrane, cholesterol increases membrane packing, reducing membrane fluidity. On the contrary, when cholesterol intercalates in a microenvironment rich in saturated fatty acids it promotes the relaxation of membrane structure, thus increasing membrane permeability and fusogenicity [18].…”

Section: Spermatozoa Membrane Dynamicsmentioning

confidence: 99%

Membrane Dynamics of Spermatozoa during Capacitation: New Insight in Germ Cells Signalling

Bernabò¹,

Ramal-Sanchez²,

Valbonetti³

et al. 2018

Germ Cell

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The study of germline stem cells and of germline cells has deep implications for the understanding of fertility, development and cancer. Nowadays, we are experiencing the very fascinating challenge of application of -OMICS technologies to this issue, which is opening new and unexpected horizons in virtually all the branches of biology. Here, we carried out a review of signalling systems involved in maturation of male germ cells and in the process that leads them to become fully fertile. In particular, we discuss the control mechanisms involved in capacitation and acrosome reaction that act at membrane level. Indeed, spermatozoa membranes play key roles in determining the achievement of fertility: they are the interface with the surrounding environment, they locate the signal transduction systems and they are active in recognizing and binding the oocyte. In addition, we discuss the effect of several compounds that could exert a negative effect on reproductive activity, by interfering with the endocrine axis, the so-called endocrine disruptors.

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“…Here, the lipid composition of spermatozoa membrane changes, due to the modification of cholesterol/phospholipids ratio [Botto et al 2010a;Gadella, and Luna 2014;Gadella and Harrison 2002]. This causes the increase in membrane disorder and isotropy, with the consequence of an increased ability of plasma membrane (PM) and outer acrosome membrane (OAM) to fuse, when it meets the oocyte zona pellucida [Gadella, and Harrison 2002;Barboni et al 2011].…”

Section: Introductionmentioning

confidence: 99%

The maturation of murine spermatozoa membranes within the epididymis, a computational biology perspective

Bernabò

Agostino

Ordinelli

et al. 2016

Systems Biology in Reproductive Medicine

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(2016) The maturation of murine spermatozoa membranes within the epididymis, a computational biology perspective, Systems Biology in Reproductive Medicine, 62:5, 299-308, DOI: 10.1080/19396368.2016 To link to this article: https://doi.org/10. 1080/19396368.2016.1205679 To become fertile, mammalian spermatozoa require completing a complex biochemical maturation that begins in the testis and ends within the female oviduct. Here, we paid attention to the events occurring at the membrane level during the epididymal transit. Indeed, in the epididymis, the molecular composition and the physical-chemical proprieties of sperm membranes markedly change, with functional cross talking among the spermatozoa, the epithelium, and the luminal content (particularly the epididymosomes). To study this process, we undertook a biological networks study, representing the involved molecules as nodes and their interactions as links. The analysis of network topology revealed that it has a scale free and small world architecture and it is robust against random failure. That assures a fast and efficient transmission of information and it leads to identifying the molecules exerting a higher level of control on the system, among which cholesterol plays a pivotal role. The reactome enrichment analysis allowed the reconstruction of the biochemical pathways involved in sperm epididymal maturation and STRING analysis permitted the identification of molecular events possibly involved in that process. In conclusion, this approach allows inferring interesting information, thus contributing to the knowledge on this process and suggesting staring points for further research. ARTICLE HISTORY

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Cell biology and functional dynamics of the mammalian sperm surface

Cited by 72 publications

References 117 publications

Oviductosome-Sperm Membrane Interaction in Cargo Delivery

Oviductosome-Sperm Membrane Interaction in Cargo Delivery

Membrane Dynamics of Spermatozoa during Capacitation: New Insight in Germ Cells Signalling

The maturation of murine spermatozoa membranes within the epididymis, a computational biology perspective

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