Cell biological mechanisms regulating chick neurogenesis

Kasioulis, Ioannis; Storey, Kate G.

doi:10.1387/ijdb.170268ks

Cited by 10 publications

(12 citation statements)

References 90 publications

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“…5A). Under normal conditions, only newborn neurons lose their apical attachment (Kasioulis and Storey, 2018), suggesting that Lzts1 in the IZ is involved in the control of newborn neuron delamination. Immunostaining with Sox2 and Tuj1 antibodies ( progenitor and neuronal markers, respectively) (Fig.…”

Section: Lzts1 Controls the Delamination Of Newborn Neurons In The Trunk Neural Tubementioning

confidence: 99%

“…Correct spatial organization of the neural tube along the medio-lateral axis requires timely detachment of newborn neurons from the apical surface in order to exit this proliferative zone and begin the morphological reorganization that underlies neuronal differentiation. This apical detachment process is known as delamination (Kasioulis and Storey, 2018). Although it has been recently shown that the synchronization of the delamination is controlled by the Notch pathway (Baek et al, 2018), molecular mechanisms controlling the timing of the delamination are not fully understood.…”

Section: Introductionmentioning

confidence: 99%

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HoxB genes regulate neuronal delamination in the trunk neural tube by controlling the expression of Lzts1

et al. 2021

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Differential Hox gene expression is central for specification of axial neuronal diversity in the spinal cord. Here, we uncover an additional function of Hox proteins in the developing spinal cord, restricted to B cluster Hox genes. We found that members of the HoxB cluster are expressed in the trunk neural tube of chicken embryo earlier than Hox from the other clusters, with poor antero-posterior axial specificity and with overlapping expression in the intermediate zone (IZ). Gain-of-function experiments of HoxB4, HoxB8 and HoxB9, respectively representative of anterior, central, and posterior HoxB genes, resulted in ectopic progenitor cells in the mantle zone. The search for HoxB8 downstream targets in the early neural tube identified the Leucine Zipper Tumor Suppressor 1 gene (Lzts1), whose expression is also activated by HoxB4 and HoxB9. Gain and loss of function experiments showed that Lzts1, expressed endogenously in the IZ, controls neuronal delamination. These data collectively indicate that HoxB genes have a generic function in the developing spinal cord, controlling the expression of Lzts1 and neuronal delamination.

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Section: Lzts1 Controls the Delamination Of Newborn Neurons In The Trunk Neural Tubementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

HoxB genes regulate neuronal delamination in the trunk neural tube by controlling the expression of Lzts1

et al. 2021

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“…Importantly, the transcription factor cascade that drives neuronal differentiation regulates the expression of Cadherins (Itoh et al, 2013;Rousso et al, 2012), reduction of which impacts adherens junctions and the linked intra-cellular actin network in the apical endfoot. These steps prefigure the delamination of newborn neurons from the ventricular surface in a process that involves local actin and microtubule cytoskeletal re-configuration (Camargo Ortega et al, 2019;Kasioulis and Storey, 2018;Kawaguchi, 2020) and in the chick and mouse neural tube, abscission of the apical membrane and dismantling of primary cilium (Das and Storey, 2014;Kasioulis et al, 2017), reviewed in (Kasioulis and Storey, 2018;Paridaen and Huttner, 2014). The presence of apical microvilli has been documented using electron microscopy (Shepard et al, 1997(Shepard et al, , 1993Smith et al, 1982;Waterman, 1976) and immunofluorescence (Marzesco et al, 2005;Weigmann et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

A lateral protrusion latticework connects neuroepithelial cells and is regulated during neurogenesis

Kasioulis

Dady

James

et al. 2021

Preprint

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Dynamic contacts between cells within the developing neuroepithelium are poorly understood but play important roles in cell and tissue morphology and cell signalling. Here, using live-cell imaging and electron microscopy we reveal multiple protrusive structures in neuroepithelial apical endfeet of the chick embryonic spinal cord, including sub-apical protrusions that extend laterally within the tissue, and observe similar structures in human neuroepithelium. We characterise the dynamics, shape, and cytoskeleton of these lateral protrusions and distinguish these structures from cytonemes/filopodia and tunnelling nanotubes. We demonstrate that lateral protrusions form a latticework of membrane contacts between non-adjacent cells, depend on actin but not microtubule dynamics and provide a lamellipodial-like platform for further extending fine actin-dependent filipodia. We find that lateral protrusions depend on the actin-binding protein WAVE1: mutant-WAVE1 misexpression attenuated protrusion and generated a round-ended apical endfoot morphology. However, this did not alter apico-basal cell polarity nor reduce tissue integrity. During normal neuronal delamination sub-apical protrusions were withdrawn, but mutant-WAVE1-induced precocious protrusion loss was insufficient to trigger neurogenesis. This study uncovers a new form of cell-cell contact within the developing neuroepithelium regulation of which prefigures neuronal delamination.

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“…Cadherins play essential roles in cell sorting and tissue formation during development (Halbleib and Nelson, 2006). Similarly, a vital role for cadherins has been demonstrated in neural crest formation, spinal cord neurogenesis, and multiple phases of brain development (Dady and Duband, 2017;Kasioulis and Storey, 2018;Veeraval et al, 2020). Processes such as neurogenesis, migration, axon guidance, and synapse formation must be coordinated to ensure the proper wiring of neural circuits.…”

Section: Introductionmentioning

confidence: 99%

Cadherin-12 Regulates Neurite Outgrowth Through the PKA/Rac1/Cdc42 Pathway in Cortical Neurons

Guo

Huang

et al. 2021

Front. Cell Dev. Biol.

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Cadherins play an important role in tissue homeostasis, as they are responsible for cell-cell adhesion during embryogenesis, tissue morphogenesis, and differentiation. In this study, we identified Cadherin-12 (CDH12), which encodes a type II classical cadherin, as a gene that promotes neurite outgrowth in an in vitro model of neurons with differentiated intrinsic growth ability. First, the effects of CDH12 on neurons were evaluated via RNA interference, and the results indicated that the knockdown of CDH12 expression restrained the axon extension of E18 neurons. The transcriptome profile of neurons with or without siCDH12 treatment revealed a set of pathways positively correlated with the effect of CDH12 on neurite outgrowth. We further revealed that CDH12 affected Rac1/Cdc42 phosphorylation in a PKA-dependent manner after testing using H-89 and 8-Bromo-cAMP sodium salt. Moreover, we investigated the expression of CDH12 in the brain, spinal cord, and dorsal root ganglia (DRG) during development using immunofluorescence staining. After that, we explored the effects of CDH12 on neurite outgrowth in vivo. A zebrafish model of CDH12 knockdown was established using the NgAgo-gDNA system, and the vital role of CDH12 in peripheral neurogenesis was determined. In summary, our study is the first to report the effect of CDH12 on axonal extension in vitro and in vivo, and we provide a preliminary explanation for this mechanism.

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Cell biological mechanisms regulating chick neurogenesis

Cited by 10 publications

References 90 publications

HoxB genes regulate neuronal delamination in the trunk neural tube by controlling the expression of Lzts1

HoxB genes regulate neuronal delamination in the trunk neural tube by controlling the expression of Lzts1

A lateral protrusion latticework connects neuroepithelial cells and is regulated during neurogenesis

Cadherin-12 Regulates Neurite Outgrowth Through the PKA/Rac1/Cdc42 Pathway in Cortical Neurons

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