Cell behavior on microparticles with different surface morphology

Huang, Sha; Fu, Xiaobing

doi:10.1016/j.jallcom.2009.12.065

Cited by 17 publications

(12 citation statements)

References 28 publications

(26 reference statements)

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“…In this case, different specifications should be taken into account, so other important aspects could be considered, such as the control of cell behavior onto the surface of the particles or the degradation profile. Furthermore, parameters such as pore interconnectivity,20, 26, 27 surface topography,28 surface chemistry, or particle size must be considered, which usually have different requirements in pure drug delivery systems.…”

Section: Methods For the Preparation Of Microparticles For Tissue Engmentioning

confidence: 99%

“…To obtain cell‐induced aggregation of microparticles, PNIPAAm has been grafted with acrylic acid to control microparticle syneresis—a time‐dependent shrinkage effect on thermoresponsive polymer hydrogels—which was proven to affect cell viability and proliferation, compromising the binding of the microparticles by cells 127. Also, cell‐induced aggregates were obtained using particles from a recombinant elastinlike polymer (ELP) in a study about the influence of particle crosslinking in the formation of aggregates 128. The studied osteoblastlike cell line was sensitive to the variation of crosslinking degree of the particles, showing ability to form aggregates only in the highest crosslinking condition.…”

Section: Applications Of Microparticles In Tissue Engineering and Regmentioning

confidence: 99%

“…Among natural polymers, gelatin microcarriers are the most commonly used for TE. In the majority of the cases, commercially available gelatin microbeads are chosen in this context as their cytotoxicity is already studied, production protocols are already optimized, and there is a wide availability of different sizes and porosities 28, 129–131. Chitosan particles have also been prepared by several processing techniques such as emulsion, spray drying, and ES 27, 132, 133.…”

Section: Applications Of Microparticles In Tissue Engineering and Regmentioning

confidence: 99%

“…Surface properties of the microparticles also influence cell behavior. For example, surface roughness and general topography influence the ability for cells to attach and proliferate 28. For example, gelatin particles with diameter ranging from 280 μm to 350 μm were fabricated by cryogenic freeze drying and modified by incorporation of bFGF 143.…”

Section: Applications Of Microparticles In Tissue Engineering and Regmentioning

confidence: 99%

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Polymer‐based microparticles in tissue engineering and regenerative medicine

Oliveira

2011

Biotechnology Progress

147

105

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Different types of biomaterials, processed into different shapes, have been proposed as temporary support for cells in tissue engineering (TE) strategies. The manufacturing methods used in the production of particles in drug delivery strategies have been adapted for the development of microparticles in the fields of TE and regenerative medicine (RM). Microparticles have been applied as building blocks and matrices for the delivery of soluble factors, aiming for the construction of TE scaffolds, either by fusion giving rise to porous scaffolds or as injectable systems for in situ scaffold formation, avoiding complicated surgery procedures. More recently, organ printing strategies have been developed by the fusion of hydrogel particles with encapsulated cells, aiming the production of organs in in vitro conditions. Mesoscale self-assembly of hydrogel microblocks and the use of leachable particles in three-dimensional (3D) layer-by-layer (LbL) techniques have been suggested as well in recent works. Along with innovative applications, new perspectives are open for the use of these versatile structures, and different directions can still be followed to use all the potential that such systems can bring. This review focuses on polymeric microparticle processing techniques and overviews several examples and general concepts related to the use of these systems in TE and RE applications. The use of materials in the development of microparticles from research to clinical applications is also discussed.

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Section: Methods For the Preparation Of Microparticles For Tissue Engmentioning

confidence: 99%

Section: Applications Of Microparticles In Tissue Engineering and Regmentioning

confidence: 99%

Section: Applications Of Microparticles In Tissue Engineering and Regmentioning

confidence: 99%

Section: Applications Of Microparticles In Tissue Engineering and Regmentioning

confidence: 99%

See 2 more Smart Citations

Polymer‐based microparticles in tissue engineering and regenerative medicine

Oliveira

2011

Biotechnology Progress

147

105

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“…Previous research has reported that the surface characteristics of microparticles do not affect cell attachment, proliferation, or uniform distribution in cells. 42 …”

Section: Optimization Of Process Parametersmentioning

confidence: 99%

Development of biodegradable methylprednisolone microparticles for treatment of articular pathology using a spray-drying technique

Tobar-Grande¹,

Godoy²,

Bustos³

et al. 2013

IJN

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Abstract:In this work, microparticles were prepared by spray-drying using albumin, chondroitin sulfate, and hyaluronic acid as excipients to create a controlled-release methylprednisolone system for use in inflammatory disorders such as arthritis. Scanning electron microscopy demonstrated that these microparticles were almost spherical, with development of surface wrinkling as the methylprednisolone load in the formulation was increased. The methylprednisolone load also had a direct influence on the mean diameter and zeta potential of the microparticles. Interactions between formulation excipients and the active drug were evaluated by x-ray diffraction, differential scanning calorimetry, and thermal gravimetric analysis, showing limited amounts of methylprednisolone in a crystalline state in the loaded microparticles. The encapsulation efficiency of methylprednisolone was approximately 89% in all formulations. The rate of methylprednisolone release from the microparticles depended on the initial drug load in the formulation. In vitro cytotoxic evaluation using THP-1 cells showed that none of the formulations prepared triggered an inflammatory response on release of interleukin-1β, nor did they affect cellular viability, except for the 9.1% methylprednisolone formulation, which was the maximum test concentration used. The microparticles developed in this study have characteristics amenable to a therapeutic role in inflammatory pathology, such as arthritis.

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Promoted field emission and cell attachment of TiO₂ nanorods/carbon fiber with hydrogen doping induced by cold atmospheric‐pressure Ar/H₂ plasma jet

Zhu

Yin

et al. 2021

Nano Select

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Cold atmospheric‐pressure plasma jet not only modifies surface morphology but also introduces functional groups on sample surfaces. In this work, TiO2 nanorods/carbon fiber (TNCF) is hydrothermally prepared, and hydrogen‐doped TNCF (H:TNCF) is obtained by the surface treatment of TNCF using cold atmospheric‐pressure Ar/H2 Plasma jet. H and OH spectral lines are observed from the emitting spectrum of atmospheric‐pressure Ar/H2 plasma jet. Nanoscaled protrusions appear at top of TiO2 nanorods after the surface treatment of cold atmospheric‐pressure Ar/H2 plasma jet. OH functional groups form on the surface of H:TNCF, based on the results of FTIR, Raman and XPS analysis. Furthermore, hydrogen doping can lower the band gap from 3.2 eV of TNCF to 2.9 eV of H:TNCF, and reduce the work function from 4.97 eV of TNCF to 4.71 eV of H:TNCF. Field emission property and cell compatibility of TNCF and H:TNCF are investigated. Field emission property of TNCF is considerably improved by hydrogen doping, due to a reduced work function and a higher field enhancement factor. In the cell attachment experiments, H:TNCF exhibits more attached cells with a larger cell attachment area, compared with the TNCF sample. The obtained results show H:TNCF prepared by hydrothermal method combined with cold atmospheric‐pressure Ar/H2 Plasma jet will be a promising candidate for field emission and biocompatibility applications.

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Cell behavior on microparticles with different surface morphology

Cited by 17 publications

References 28 publications

Polymer‐based microparticles in tissue engineering and regenerative medicine

Polymer‐based microparticles in tissue engineering and regenerative medicine

Development of biodegradable methylprednisolone microparticles for treatment of articular pathology using a spray-drying technique

Promoted field emission and cell attachment of TiO₂ nanorods/carbon fiber with hydrogen doping induced by cold atmospheric‐pressure Ar/H₂ plasma jet

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Cell behavior on microparticles with different surface morphology

Cited by 17 publications

References 28 publications

Polymer‐based microparticles in tissue engineering and regenerative medicine

Polymer‐based microparticles in tissue engineering and regenerative medicine

Development of biodegradable methylprednisolone microparticles for treatment of articular pathology using a spray-drying technique

Promoted field emission and cell attachment of TiO2 nanorods/carbon fiber with hydrogen doping induced by cold atmospheric‐pressure Ar/H2 plasma jet

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Product

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Promoted field emission and cell attachment of TiO₂ nanorods/carbon fiber with hydrogen doping induced by cold atmospheric‐pressure Ar/H₂ plasma jet