Cell-based treatment of osteochondral defects in the rabbit knee with natural and synthetic matrices: cellular seeding determines the outcome

Rudert, Maximilian; Wilms, U.; Hoberg, Maik; Wirth, Carl Joachim

doi:10.1007/s00402-005-0008-2

Cited by 26 publications

(24 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Several studies on cell-based treatment of cartilage defects with different scaffolds implanted in a rabbit model have utilized defect sizes of 3-4 mm. 21,26,27 Hiraki et al showed with the administration of fibroblast growth factor-2 for 2 weeks that it induces a successful cartilaginous repair response in 5-mm full-thickness articular cartilage defects in rabbits. 28 Although our in vivo study showed only early stages of the repair process, there is the possibility that the large cartilage defects could be repaired without any addition of growth factors.…”

Section: Discussionmentioning

confidence: 98%

Transplantation of Allogeneic Chondrocytes Cultured in Fibroin Sponge and Stirring Chamber to Promote Cartilage Regeneration

Chueh

Tomita²,

Yamamoto³

et al. 2007

Tissue Engineering

View full text Add to dashboard Cite

Cartilage regeneration using a fibroin sponge and a stirring chamber was investigated to improve the potential of articular cartilage tissue engineering. Chondrocytes seeded on the fibroin-sponge scaffolds were cultured in the stirring chamber (a bioreactor facilitating mechanical stimulation) for up to 3 weeks. Changes in DNA content, glycosaminoglycan (GAG) amount, integrin subunits alpha5 and beta1 fluorescence intensity, and morphologic appearance, were studied to evaluate tissue maturity. Seeded scaffolds subjected to the stirring chamber demonstrated significant increases in both DNA content (38.9%) and GAG content (54.3%) at day 21 compared to the control group. In addition, the stirring chamber system facilitated a maturation of cartilage tissue showed by histologic examination, after a staining of proteoglycan and type II collagen. Clinical feasibility of the fibroin and stirring chamber system was evaluated using rabbit models with cartilage defect. Large defects on rabbit knee joints were repaired with regenerated cartilage, which resembles hyaline cartilage at 12 weeks after operation. These studies demonstrated the potential of such mechanically stimulated scaffold/cell constructs to support chondrogenesis in vivo.

show abstract

Section: Discussionmentioning

confidence: 98%

Transplantation of Allogeneic Chondrocytes Cultured in Fibroin Sponge and Stirring Chamber to Promote Cartilage Regeneration

Chueh

Tomita²,

Yamamoto³

et al. 2007

Tissue Engineering

View full text Add to dashboard Cite

show abstract

“…A housekeeping enzyme GADPH is shown as reference level of gene expression into a three-dimensional scaffold [3,11,21,22]. Most of these scaffolds are solid preformed structures often made by fibrous meshes or porous foam.…”

Section: Discussionmentioning

confidence: 99%

Cartilage repair with chondrocytes in fibrin hydrogel and MPEG polylactide scaffold: an in vivo study in goats

Lind

Larsen²,

Clausen³

et al. 2008

Knee Surg Sports Traumatol Arthr

View full text Add to dashboard Cite

Polylactic acid polymers have been used extensively as biomaterials and have shown promising properties for cartilage tissue engineering. Numerous scaffold materials exist and the optimal scaffold needs to be identified. We have tried to assess the possibilities for cartilage repair by the use of two different scaffold techniques; autologous chondrocytes in a fibrin hydrogel and a novel MPEG-PLGA scaffold, where autologous chondrocytes are immobilized within the MPEG-PLGA scaffold by a fibrin hydrogel. Twenty adult goats were used for the study. A 6 mm circular full-thickness cartilage defect was created in both medial femoral condyles. The defects were randomized to the following four treatment groups. (1) Empty defect (control). (2) Subchondral drilling (control). (3) Fibrin hydrogel with autologous chondrocytes. (4) Fibrin hydrogel/chondrocyte solution in a MPEG-PLGA porous scaffold. Animals were followed for 4 month. Eight defects in each treatment group completed the study. ICRS macroscopic scoring (0-12). Indentation test was performed to assess stiffness of repair tissue. Histological analyses was performed using O'Driscoll and Pineda cartilage scores as well as percentage tissue filling of the defects. The MPEG-PLGA/chondrocytes scaffold was the superior treatment modality based on the macroscopic surface score, histological scores and defect filling. The mechanical test demonstrated no difference between treatment groups. The MPEG-PLGA/chondrocyte composite demonstrated significantly better cartilage repair response than empty defects, osteochondral drilling and fibrin hydrogel with chondrocytes. The novel MPEG-PLGA scaffold in combination with chondrocytes need further studies with respect to longer follow-up times.

show abstract

“…4,92,93 Several studies have shown that new cartilage can be engineered in vivo by transplanting chondrocytes seeded into a threedimensional scaffold. 4,[94][95][96] Rodent models offer advantages for mechanistic study, evaluation of allogenic and xenogenic strategies, and initial feasibility studies. To demonstrate efficacy and safety before human clinical use, long-term large animal studies evaluating a new treatment in full-thickness cartilage lesions of the weight-bearing areas are needed.…”

Section: Discussionmentioning

confidence: 99%

Animal Models for Cartilage Regeneration and Repair

Chu

Szczodry

Bruno

2010

Tissue Engineering Part B: Reviews

441

451

View full text Add to dashboard Cite

cartilage injury and degeneration are leading causes of disability. Animal studies are critically important to developing effective treatments for cartilage injuries. This review focuses on the use of animal models for the study of the repair and regeneration of focal cartilage defects. Animals commonly used in cartilage repair studies include murine, lapine, canine, caprine, porcine, and equine models. There are advantages and disadvantages to each model. Small animal rodent and lapine models are cost effective, easy to house, and useful for pilot and proof-of-concept studies. The availability of transgenic and knockout mice provide opportunities for mechanistic in vivo study. Athymic mice and rats are additionally useful for evaluating the cartilage repair potential of human cells and tissues. Their small joint size, thin cartilage, and greater potential for intrinsic healing than humans, however, limit the translational value of small animal models. Large animal models with thicker articular cartilage permit study of both partial thickness and full thickness chondral repair, as well as osteochondral repair. Joint size and cartilage thickness for canine, caprine, and mini-pig models remain significantly smaller than that of humans. The repair and regeneration of chondral and osteochondral defects of size and volume comparable to that of clinically significant human lesions can be reliably studied primarily in equine models. While larger animals may more closely approximate the human clinical situation, they carry greater logistical, financial, and ethical considerations. A multifactorial analysis of each animal model should be carried out when planning in vivo studies. Ultimately, the scientific goals of the study will be critical in determining the appropriate animal model.

show abstract

Cell-based treatment of osteochondral defects in the rabbit knee with natural and synthetic matrices: cellular seeding determines the outcome

Abstract: There is no difference in the outcome between the three tested matrices in the treatment of osteochondral defects in the rabbit knee. The results of this in vitro experiment are promising and with refinement may lead to useful clinical therapies.

Cited by 26 publications

References 50 publications

Transplantation of Allogeneic Chondrocytes Cultured in Fibroin Sponge and Stirring Chamber to Promote Cartilage Regeneration

Transplantation of Allogeneic Chondrocytes Cultured in Fibroin Sponge and Stirring Chamber to Promote Cartilage Regeneration

Cartilage repair with chondrocytes in fibrin hydrogel and MPEG polylactide scaffold: an in vivo study in goats

Animal Models for Cartilage Regeneration and Repair

Contact Info

Product

Resources

About