Cell Based Therapy for Chronic Liver Disease: Role of Fetal Liver Cells in Restoration of the Liver Cell Functions

Tripura, Chaturvedula; Khan, Aleem Ahmed; Pande, Gopal

doi:10.5772/45876

Cited by 2 publications

(2 citation statements)

References 109 publications

(109 reference statements)

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“…Several clinical studies have reported the successful use of autologous BM cells in conditions of liver cirrhosis and liver carcinoma (reviewed in [82]). G-CSF-mediated direct mobilization of CD34 + cells from the BM to the affected organ has also shown to improve the survival in liver failure conditions [83].…”

Section: Clinical Applications Of Hsctmentioning

confidence: 99%

Applications of Human Hematopoietic Stem Cells Isolated and Expanded from Different Tissues in Regenerative Medicine

Tripura

Pande

2013

Regen. Med.

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Bone marrow transplantation is a well-established stem cell-based therapy for the management of malignant and nonmalignant hematological disorders. In addition to the bone marrow, therapeutic hematopoietic stem cells (HSCs) can also be obtained from umbilical cord blood and mobilized peripheral blood. Transplantation of HSCs isolated from these tissues can be carried out with or without prior enrichment of specific cell types. New methodologies have been developed for lineage-specific HSC expansion and their transplantation as a supplementary treatment to whole bone marrow transplantation. In this review we have described the current methodologies for isolating and processing HSCs from various tissues, and discussed strategies to generate sufficient and functional HSCs for clinical and preclinical applications by expansion ex vivo. The various disease conditions in which these cells could be used, and the methods for delivering the cells into patients, are also discussed.

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Section: Clinical Applications Of Hsctmentioning

confidence: 99%

Applications of Human Hematopoietic Stem Cells Isolated and Expanded from Different Tissues in Regenerative Medicine

Tripura

Pande

2013

Regen. Med.

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“…Cell therapy is an emerging technology and has shown significant promise in addressing the current demand for alternative options to liver transplantation to improve liver functions and act as a supportive bridge therapy in CLD[ 1 , 2 ]. However, it is necessary to ascertain appropriate cell types from widely acceptable sources and resolve several unanswered questions before utilizing cell-based technology in the clinical setting.…”

Section: Introductionmentioning

confidence: 99%

Long-term and non-invasive in vivo tracking of DiD dye-labeled human hepatic progenitors in chronic liver disease models

Tripura¹,

Srinivas²,

Vishwakarma³

et al. 2022

World J Hepatol

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BACKGROUND Chronic liver diseases (CLD) are the major public health burden due to the continuous increasing rate of global morbidity and mortality. The inherent limitations of organ transplantation have led to the development of stem cell-based therapy as a supportive and promising therapeutic option. However, identifying the fate of transplanted cells in vivo represents a crucial obstacle. AIM To evaluate the potential applicability of DiD dye as a cell labeling agent for long-term, and non-invasive in vivo tracking of transplanted cells in the liver. METHODS Magnetically sorted, epithelial cell adhesion molecule positive (1 × 10 6 cells/mL) fetal hepatic progenitor cells were labeled with DiD dye and transplanted into the livers of CLD-severe combined immunodeficiency (SCID) mice. Near-infrared (NIR) imaging was performed for in vivo tracking of the DiD-labeled transplanted cells along with colocalization of hepatic markers for up to 80 d. The existence of human cells within mouse livers was identified using Alu polymerase chain reaction and sequencing. RESULTS NIR fluorescence imaging of CLD-SCID mice showed a positive fluorescence signal of DiD at days 7, 15, 30, 45, 60, and 80 post-transplantation. Furthermore, positive staining of cytokeratin, c-Met, and albumin colocalizing with DiD fluorescence clearly demonstrated that the fluorescent signal of hepatic markers emerged from the DiD-labeled transplanted cells. Recovery of liver function was also observed with serum levels of glutamic-oxaloacetic transaminase, glutamate-pyruvate transaminase, and bilirubin. The detection of human-specific Alu sequence from the transplanted mouse livers provided evidence for the survival of transplanted cells at day 80. CONCLUSION DiD-labeling is promising for long-term and non-invasive in vivo cell tracking, and understanding the regenerative mechanisms incurred by the transplanted cells.

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Cell Based Therapy for Chronic Liver Disease: Role of Fetal Liver Cells in Restoration of the Liver Cell Functions

Cited by 2 publications

References 109 publications

Applications of Human Hematopoietic Stem Cells Isolated and Expanded from Different Tissues in Regenerative Medicine

Applications of Human Hematopoietic Stem Cells Isolated and Expanded from Different Tissues in Regenerative Medicine

Long-term and non-invasive in vivo tracking of DiD dye-labeled human hepatic progenitors in chronic liver disease models

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