Cell-based biosensors: Recent trends, challenges and future perspectives

Gupta, Niharika; Renugopalakrishnan, V.; Liepmann, Dorian; Paulmurugan, Ramasamy; Malhotra, Bansi D.

doi:10.1016/j.bios.2019.111435

Cited by 207 publications

(104 citation statements)

References 213 publications

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“…Collective cell movements differ significantly from the motion of individual cells, as cell clusters achieve locomotion via coordinated cell-cell adhesions [3–5] while singleton cells migrate largely independent of its proximal neighbors [6]. Few microfluidic systems have been adapted to study the collective behaviors of homogenous or heterogeneous cell groups [7–10] despite their wide usage in the chemotactic study of individual cells [7–11]. Microfluidic assays can significantly advance vision research by enabling quantitative study of the complex and poorly understood relationships between exogenous chemotactic fields and the collective RPC motility stimulated during retinogenesis [12–14].…”

Section: Introductionmentioning

confidence: 99%

Collective behaviors of Drosophila-derived retinal progenitors in controlled microenvironments

et al. 2019

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Collective behaviors of retinal progenitor cells (RPCs) are critical to the development of neural networks needed for vision. Signaling cues and pathways governing retinal cell fate, migration, and functional organization are remarkably conserved across species, and have been well-studied using Drosophila melanogaster. However, the collective migration of heterogeneous groups of RPCs in response to dynamic signaling fields of development remains incompletely understood. This is in large part because the genetic advances of seminal invertebrate models have been poorly complemented by in vitro cell study of its visual development. Tunable microfluidic assays able to replicate the miniature cellular microenvironments of the developing visual system provide newfound opportunities to probe and expand our knowledge of collective chemotactic responses essential to visual development. Our project used a controlled, microfluidic assay to produce dynamic signaling fields of Fibroblast Growth Factor (FGF) that stimulated the chemotactic migration of primary RPCs extracted from Drosophila. Results illustrated collective RPC chemotaxis dependent on average size of clustered cells, in contrast to the non-directional movement of individually-motile RPCs. Quantitative study of these diverse collective responses will advance our understanding of retina developmental processes, and aid study/treatment of inherited eye disease. Lastly, our unique coupling of defined invertebrate models with tunable microfluidic assays provides advantages for future quantitative and mechanistic study of varied RPC migratory responses.

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Section: Introductionmentioning

confidence: 99%

Collective behaviors of Drosophila-derived retinal progenitors in controlled microenvironments

et al. 2019

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“…The precisely-controlled and defined environments created using microfluidic systems have heralded tremendous advances in biology and regenerative medicine over the past decade [24,25,26,27], not only in technological development of single cells and high throughput microdevices [28,29], but additionally in the emergence of microfluidically-manipulated biomaterials for tissue grafts and organs on a chip [30,31,32]. Numerous groups, including our own, have developed microfluidic systems for precise analyses of neural migratory responses within defined concentration gradients of chemotactic stimuli using a variety of experimentally determined substrates and surfaces [33,34,35,36,37,38,39].…”

Section: Introductionmentioning

confidence: 99%

Invertebrate Retinal Progenitors as Regenerative Models in a Microfluidic System

Pena

Zhang

Majeska

et al. 2019

Cells

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Regenerative retinal therapies have introduced progenitor cells to replace dysfunctional or injured neurons and regain visual function. While contemporary cell replacement therapies have delivered retinal progenitor cells (RPCs) within customized biomaterials to promote viability and enable transplantation, outcomes have been severely limited by the misdirected and/or insufficient migration of transplanted cells. RPCs must achieve appropriate spatial and functional positioning in host retina, collectively, to restore vision, whereas movement of clustered cells differs substantially from the single cell migration studied in classical chemotaxis models. Defining how RPCs interact with each other, neighboring cell types and surrounding extracellular matrixes are critical to our understanding of retinogenesis and the development of effective, cell-based approaches to retinal replacement. The current article describes a new bio-engineering approach to investigate the migratory responses of innate collections of RPCs upon extracellular substrates by combining microfluidics with the well-established invertebrate model of Drosophila melanogaster. Experiments utilized microfluidics to investigate how the composition, size, and adhesion of RPC clusters on defined extracellular substrates affected migration to exogenous chemotactic signaling. Results demonstrated that retinal cluster size and composition influenced RPC clustering upon extracellular substrates of concanavalin (Con-A), Laminin (LM), and poly-L-lysine (PLL), and that RPC cluster size greatly altered collective migratory responses to signaling from Fibroblast Growth Factor (FGF), a primary chemotactic agent in Drosophila. These results highlight the significance of examining collective cell-biomaterial interactions on bio-substrates of emerging biomaterials to aid directional migration of transplanted cells. Our approach further introduces the benefits of pairing genetically controlled models with experimentally controlled microenvironments to advance cell replacement therapies.

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“…The advantages of using living microorganisms are well-known [98]: on one hand they are able to detect a wide range of substances in a broad range of operative conditions such as pH values, temperature and oxygen concentration. By the other hand the exploitation of hydrogel in this kind of devices is self-evident [99]: Fig.…”

Section: Sensors Based On Living Cellsmentioning

confidence: 99%

Progress in hydrogels for sensing applications: a review

Pinelli

Magagnin

Rossi

2020

Materials Today Chemistry

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There are several developments taking place in the field of sensors driven by the world today requirements. One of the most important novelties of the last two decades in the field is represented by the hydrogel-based sensors which constitute a wide family of innovative smart sensing devices relevant for many different applications. Hydrogels in fact are hydrophilic, biocompatible and highly water swellable polymer networks able to convert chemical energy into mechanical energy, with the great peculiarity to be able to respond to external stimuli. These characteristics have ensured them considerable recognition as valuable tool for smart sensing and diagnostics. The aim of this review is to focus on the advances obtained in the field in the last ten years.

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Cell-based biosensors: Recent trends, challenges and future perspectives

Cited by 207 publications

References 213 publications

Collective behaviors of Drosophila-derived retinal progenitors in controlled microenvironments

Collective behaviors of Drosophila-derived retinal progenitors in controlled microenvironments

Invertebrate Retinal Progenitors as Regenerative Models in a Microfluidic System

Progress in hydrogels for sensing applications: a review

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