Cell-Based Bioluminescence Screening Assays

Abstract

Drug screening is an essential and widely used technique for drug discovery in various biomedical fields notably in oncology. Here we describe a functional screening assay based on the bioluminescence detection of a secreted luciferase for monitoring cell viability of cancer cells in a high-throughput format. This assay allows the screening of large libraries comprising thousands of compounds and the identification of potential anticancer molecules in a rapid, facile, and cost-effective manner.

Patient-Derived Glioma Models: From Patients to Dish to Animals

“…To monitor activation of transcription factors/pathways such as Nuclear factor kappa-light-chain-enhancer of activated B cells (NFkB), the reporter of choice is cloned under the control of NFkB responsive elements [57]. The most effective way to engineer GSCs to express these reporters is to use lentivirus vectors, as adding the vector directly to the conditioned medium of GSCs results in >90% transduction efficiency and allows for integration of the expression cassette into the genome of cells, yielding stable expression of the reporter [59,60,61].…”