Cell-Based Assays for Neurotoxins

McNutt, Patrick; Beske, Phillip; Thirunavukkarsu, Nagarajan

doi:10.1007/978-94-007-5869-8_31

Cited by 4 publications

(2 citation statements)

References 92 publications

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“…Neurons derived from hiPSCs exhibited appropriate morphology, electrical behaviors, transsynaptic signaling and network activity. This combination of physiological relevance and neuromimetic responses enhances the relevance of the use of hiPSC-derivatives for neurotoxicology studies [ 164 ]. The appearance of network activity suggests that differentiation of hiPSCs into neuronal populations can generate synaptically mature neuronal networks.…”

Section: Emerging Cell-based Assays Using Human Ipscs Derivativesmentioning

confidence: 99%

Emerging Opportunities in Human Pluripotent Stem-Cells Based Assays to Explore the Diversity of Botulinum Neurotoxins as Future Therapeutics

Lamotte

Perrier

Martinat

et al. 2021

IJMS

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Botulinum neurotoxins (BoNTs) are produced by Clostridium botulinum and are responsible for botulism, a fatal disorder of the nervous system mostly induced by food poisoning. Despite being one of the most potent families of poisonous substances, BoNTs are used for both aesthetic and therapeutic indications from cosmetic reduction of wrinkles to treatment of movement disorders. The increasing understanding of the biology of BoNTs and the availability of distinct toxin serotypes and subtypes offer the prospect of expanding the range of indications for these toxins. Engineering of BoNTs is considered to provide a new avenue for improving safety and clinical benefit from these neurotoxins. Robust, high-throughput, and cost-effective assays for BoNTs activity, yet highly relevant to the human physiology, have become indispensable for a successful translation of engineered BoNTs to the clinic. This review presents an emerging family of cell-based assays that take advantage of newly developed human pluripotent stem cells and neuronal function analyses technologies.

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Section: Emerging Cell-based Assays Using Human Ipscs Derivativesmentioning

confidence: 99%

Emerging Opportunities in Human Pluripotent Stem-Cells Based Assays to Explore the Diversity of Botulinum Neurotoxins as Future Therapeutics

Lamotte

Perrier

Martinat

et al. 2021

IJMS

View full text Add to dashboard Cite

show abstract

“…Over the past 5 years, assays for several botulinum toxins based on cell levels have been established, with the potency of botulinum toxins quantitatively evaluated with a similar or higher tolerance than the mouse bioassay (Pellett, 2013). Stem celland neurogenic cell line-based assays have been used for the identification of biological activities of the botulinum toxins (Maslanka et al, 2011;McNutt et al, 2013;Thirunavukkarasu et al, 2018). Stem cell-or neurogenic cell line-based assays offer comparable sensitivity to MLB for the detection of botulinum toxin.…”

Section: Cell-based Assaymentioning

confidence: 99%

Alternative Methods for Testing Botulinum Toxin: Current Status and Future Perspectives

Nepal

Jeong

2020

Biomolecules & Therapeutics

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Clostridium botulinum is an anaerobic, gram-positive, sporeforming, rod-shaped bacterium that produces neurotoxic proteins called botulinum toxins (Nigam and Nigam, 2010). Foodborne poisoning cases of botulinum toxins were first observed in eighteenth-century Europe, and the condition was termed 'sausage poisoning' or botulism as 'Botulus' means sausage in Latin (Kerner, 1817). Depending on the type of illness caused by botulinum toxins, C. botulinum strains are divided into four different groups. Bacterial groups I and II are associated with the human illness, group III is associated with illness in animals, and group IV is not related to any illness (Nawrocki et al., 2018). So far, depending on the serological properties of the toxins, at least seven different types (A-G) of botulinum toxins have been identified from different C. botulinum strains (Nawrocki et al., 2018). Botulinum toxins A, B, and F are produced by group I bacteria, and toxins B, D, and E are produced by group II bacteria (Lindström and Korkeala, 2006). Botulinum toxin types A, B, and E have been identified as the most common neurotoxins causing human poisoning, whereas toxin types C and D are rarely associated with human toxicities; type F causes minimal human toxicity (Hodowanec and Bleck, 2015). In addition to C. botulinum, several other strains of bacteria can produce botulinum toxins, e.g.

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Critical analysis in the advancement of cell-based assays for botulinum neurotoxin

Ambrin

Cai

Singh³

2022

Critical Reviews in Microbiology

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Cell-Based Assays for Neurotoxins

Cited by 4 publications

References 92 publications

Emerging Opportunities in Human Pluripotent Stem-Cells Based Assays to Explore the Diversity of Botulinum Neurotoxins as Future Therapeutics

Emerging Opportunities in Human Pluripotent Stem-Cells Based Assays to Explore the Diversity of Botulinum Neurotoxins as Future Therapeutics

Alternative Methods for Testing Botulinum Toxin: Current Status and Future Perspectives

Critical analysis in the advancement of cell-based assays for botulinum neurotoxin

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