Cell-autonomous-like silencing of GFP-partitioned transgenic Nicotiana benthamiana

Sohn, Seong-Han; Frost, Jennifer M.; Kim, Chongam; Choi, Seung-Kook; Lee, Yi; Seo, Mi-Suk; Lim, Sung-Hun; Choi, Yeonhee; Kim, Kook‐Hyung; Lomonossoff, George P.

doi:10.1093/jxb/eru200

Cited by 7 publications

(3 citation statements)

References 52 publications

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“…(Voinnet et al ; Tournier et al ; Molnar et al ; Bai et al ). While unidirectionality (Palauqui et al ; Sohn et al ) or directionality‐depended efficiency of silencing spread (Molnar et al ) have been reported, other factors might have an impact on the silencing spread efficiency, such as the age or the developmental stage of the plants (Liang et al ), or the grafting method (Crete et al ).…”

Section: Identity and Movement Of The Mobile Signalmentioning

confidence: 99%

RNA silencing movement in plants

Mermigka

Verret

Kalantidis

2015

JIPB

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Multicellular organisms, like higher plants, need to coordinate their growth and development and to cope with environmental cues. To achieve this, various signal molecules are transported between neighboring cells and distant organs to control the fate of the recipient cells and organs. RNA silencing produces cell non-autonomous signal molecules that can move over short or long distances leading to the sequence specific silencing of a target gene in a well defined area of cells or throughout the entire plant, respectively. The nature of these signal molecules, the route of silencing spread, and the genes involved in their production, movement and reception are discussed in this review. Additionally, a short section on features of silencing spread in animal models is presented at the end of this review.

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Section: Identity and Movement Of The Mobile Signalmentioning

confidence: 99%

RNA silencing movement in plants

Mermigka

Verret

Kalantidis

2015

JIPB

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“…Sohn et al 58 published a whole transcriptome analysis in silenced and non-silenced leaf regions of a N. benthamiana transgenic line exhibiting local cell-autonomous leaf-partitioned GFP silencing achieved through both PTGS and TGS mechanisms. In this study, leaf region-specific DEGs were three times more abundant in non-silenced than in silenced regions.…”

Section: Discussionmentioning

confidence: 99%

Stress-related transcriptomic changes associated with GFP transgene expression and active transgene silencing in plants

Kallemi,

Verret,

Andronis

et al. 2024

Sci Rep

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Plants respond to biotic and abiotic stress by activating and interacting with multiple defense pathways, allowing for an efficient global defense response. RNA silencing is a conserved mechanism of regulation of gene expression directed by small RNAs important in acquired plant immunity and especially virus and transgene repression. Several RNA silencing pathways in plants are crucial to control developmental processes and provide protection against abiotic and biotic stresses as well as invasive nucleic acids such as viruses and transposable elements. Various notable studies have shed light on the genes, small RNAs, and mechanisms involved in plant RNA silencing. However, published research on the potential interactions between RNA silencing and other plant stress responses is limited. In the present study, we tested the hypothesis that spreading and maintenance of systemic post-transcriptional gene silencing (PTGS) of a GFP transgene are associated with transcriptional changes that pertain to non-RNA silencing-based stress responses. To this end, we analyzed the structure and function of the photosynthetic apparatus and conducted whole transcriptome analysis in a transgenic line of Nicotiana benthamiana that spontaneously initiates transgene silencing, at different stages of systemic GFP-PTGS. In vivo analysis of chlorophyll a fluorescence yield and expression levels of key photosynthetic genes indicates that photosynthetic activity remains unaffected by systemic GFP-PTGS. However, transcriptomic analysis reveals that spreading and maintenance of GFP-PTGS are associated with transcriptional reprogramming of genes that are involved in abiotic stress responses and pattern- or effector-triggered immunity-based stress responses. These findings suggest that systemic PTGS may affect non-RNA-silencing-based defense pathways in N. benthamiana, providing new insights into the complex interplay between different plant stress responses.

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“…Systemic GFP-PTGS incurs changes in whole transcriptome pro les Sohn and colleagues (2014) 59 published a whole transcriptome analysis in silenced and non-silenced leaf regions of a N. benthamiana transgenic line exhibiting local cell-autonomous leaf-partitioned GFP silencing achieved through both PTGS and TGS mechanisms. In this study, leaf region-speci c DEGs were three times more abundant in non-silenced than in silenced regions.…”

Section: Discussionmentioning

confidence: 99%

Stress-related transcriptomic changes associated with GFP transgene overexpression and active transgene silencing in plants

Kallemi

Verret

Andronis

et al. 2023

Preprint

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Plants respond to biotic and abiotic stress by activating and interacting with multiple defense pathways, allowing for an efficient global defense response. RNA silencing is a conserved mechanism of regulation of gene expression directed by small RNAs important in acquired plant immunity and especially virus and transgene repression. Several RNA silencing pathways in plants are crucial to control developmental processes and provide protection against abiotic and biotic stresses as well as invasive nucleic acids such as viruses and transposable elements. Various notable studies have shed light on the genes, small RNAs, and mechanisms involved in plant RNA silencing. However, published research on the potential interactions between RNA silencing and other plant stress responses is limited. In the present study, we tested the hypothesis that spreading and maintenance of systemic post-transcriptional gene silencing (PTGS) of a GFP transgene are associated with transcriptional changes that pertain to non-RNA silencing-based stress responses. To this end, we analyzed the structure and function of the photosynthetic apparatus and conducted whole transcriptome analysis in a transgenic line of Nicotiana benthamiana that spontaneously initiates transgene silencing, at different stages of systemic GFP-PTGS. In vivo analysis of chlorophyll a fluorescence yield and expression levels of key photosynthetic genes indicates that photosynthetic activity remains unaffected by systemic GFP-PTGS. However, transcriptomic analysis reveals that spreading and maintenance of GFP-PTGS are associated with transcriptional reprogramming of genes that are involved in abiotic stress responses and pattern- or effector-triggered immunity-based stress responses. These results suggest that systemic PTGS may interact with non-RNA silencing-based defense pathways in N. benthamiana.

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Cell-autonomous-like silencing of GFP-partitioned transgenic Nicotiana benthamiana

Cited by 7 publications

References 52 publications

RNA silencing movement in plants

RNA silencing movement in plants

Stress-related transcriptomic changes associated with GFP transgene expression and active transgene silencing in plants

Stress-related transcriptomic changes associated with GFP transgene overexpression and active transgene silencing in plants

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