Cell-attachment activities of surface immobilized oligopeptides RGD, RGDS, RGDV, RGDT, and YIGSR toward five cell lines

Hirano, Yoshiaki; Okuno, Motoya; Hayashi, Toshio; Gotō, Kunio; Nakajima, Akira

doi:10.1163/156856293x00546

Cited by 82 publications

(52 citation statements)

References 29 publications

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“…To test this hypothesis and evaluate the specificity of the RGD peptide in cell attachment, HUES7 cells were seeded on surface bound GRGDSP peptide in serum-free conditions. MEF cells, which were used to test surface modification, showed an increase in cell attachment and spreading on RGD-modified samples, which is consistent with previously published data with fibroblast cell types on RGD-modified synthetic surfaces [33][34][35][36]. However, HUES7 cells failed to attach on RGD peptide-modified glass surfaces.…”

Section: Discussionsupporting

confidence: 89%

Characterization of the interface between adsorbed fibronectin and human embryonic stem cells

Kalaskar

Downes

Murray

et al. 2013

J. R. Soc. Interface.

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The cell-substrate interface plays a key role in the regulation of cell behaviour. Defining the properties of this interface is particularly important for human embryonic stem (hES) cell culture, because changes in this environment can regulate hES cell differentiation. It has been established that fibronectincoated surfaces can promote the attachment, growth and maintenance of the undifferentiated phenotype of hES cells. We investigated the influence of the surface density of adsorbed fibronectin on hES cell behaviour in defined serum-free culture conditions and demonstrated that only 25 per cent surface saturation was required to maintain attachment, growth and maintenance of the undifferentiated phenotype. The influence of surface-adsorbed fibronectin fragments was compared with whole fibronectin, and it was demonstrated that the 120 kDa fragment central binding domain alone was able to sustain hES cells in an undifferentiated phenotype in a similar fashion to fibronectin. Furthermore, hES cell attachment to both fibronectin and the 120 kDa fragment was mediated by integrin a5b1. However, although a substrateattached synthetic arginine-glycine-aspartic acid (RGD) peptide alone was able to promote the attachment and spreading of fibroblasts, it was inactive for hES cells, indicating that stem cells have different requirements in order to attach and spread on the central fibronectin RGD-cell-binding domain. This study provides further information on the characteristics of the cellsubstrate interface required to control hES cell behaviour in clearly defined serum-free conditions, which are needed for the development of therapeutic applications of hES cells.

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Section: Discussionsupporting

confidence: 89%

Characterization of the interface between adsorbed fibronectin and human embryonic stem cells

Kalaskar

Downes

Murray

et al. 2013

J. R. Soc. Interface.

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“…[16][17][18] RGD and YIGSR peptides were immobilized to an ethylene-acrylic co-polymer film, and an enhancement of cell adhesion was achieved with some of the tested cell lines. 19 RGD peptides also were coupled covalently to poly(␥-methyl-Lglutamate) and tested successfully with respect to the adhesion of fibroblasts. 20 Networks of poly(ethylene glycol) crosslinked with acrylic acid were grafted with RGD and YIGSR peptides, and they supported fibroblast adhesion.…”

Section: Introductionmentioning

confidence: 99%

Modification of glassy carbon surfaces with synthetic laminin-derived peptides for nerve cell attachment and neurite growth

Huber

Heiduschka

Kienle

et al. 1998

J. Biomed. Mater. Res.

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Interactions between cultured nerve cells and surfaces are of importance for the implantation of biocompatible electrode materials such as glassy carbon (GC). Since implants serve as recording sensors in prosthetic neuroscience, we investigated whether coating electrodes with certain laminin derivatives containing the peptide sequences SIKVAV, CDPGYIGSR, PDSGR, YFQRYLI, and RNIAEIIKDA influences neuronal adhesion and neurite outgrowth in vitro. The coating of GC was performed by electrochemical polymerization and, for comparison, by adsorption or covalent coupling. Electrochemical polymerization is suitable for the coupling of peptides to GC, as shown by amino acid analysis and sequencing. Embryonic chicken retinal ganglion cells and brain cells (days E7 or E17) were used for both attachment and growth studies. Surfaces made by electrochemical polymerization of peptides were more efficient than those made by adsorption or covalent coupling of peptides. Synthetic cyclic peptide derivatives of CDPGYIGSR and 18-mer SIKVAV were found to be more efficient than the linear peptides. Competitive effects that resulted in a decreased cell attachment could be found upon application of soluble peptides. Nevertheless, irrespective of the method of coating, peptides were less efficient compared with the whole laminin molecule, as expected from its multiple adhesion sites. When small GC pins were implanted into the brain of E17 chicken after coating with the 18-mer SIKVAV peptide, nerve cell attachment was observed in vivo. The results suggest that chronically implantable materials may exert a higher neurocompatibility when coated with synthetic peptides.

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“…Als Ligand mit hoher Affinität zu den Integrinrezeptoren der Zellmembran trägt die RGD-Sequenz wesentlich zur Zelladhäsion an die extrazelluläre Matrix bei. In der Literatur gibt es zahlreiche Beispiele für synthetische Oberflächen, die durch die Ausrüstung mit RGD-Peptiden eine verbesserte Zelladhäsion zeigen [9][10][11][12] Die nach dem CVD-Verfahren erhaltene Amino-ppx-Beschichtung ist gegenüber gängigen Lösungsmitteln resistent und weist die für weitere Modifizierungen notwendigen Oberflächenfunktionalitäten auf. Die Schichtdicke liegt unterhalb von 500 nm.…”

Section: Introductionunclassified

Konzept zur bioaktiven Ausrüstung

Weiß¹,

Klee²,

Höcker³

2001

BIOmaterialien

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Cell-attachment activities of surface immobilized oligopeptides RGD, RGDS, RGDV, RGDT, and YIGSR toward five cell lines

Cited by 82 publications

References 29 publications

Characterization of the interface between adsorbed fibronectin and human embryonic stem cells

Characterization of the interface between adsorbed fibronectin and human embryonic stem cells

Modification of glassy carbon surfaces with synthetic laminin-derived peptides for nerve cell attachment and neurite growth

Konzept zur bioaktiven Ausrüstung

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